目的研究瑞芬太尼对开胸手术单肺通气(one lung ventilation,OLV)患者涎液化糖链蛋白6(krebs von den lungen 6,KL-6)及氧化应激的影响。方法选取2016年1月-2018年4月入住重庆市巴南区人民医院并择期开胸手术行OLV患者76例纳入研究,随...目的研究瑞芬太尼对开胸手术单肺通气(one lung ventilation,OLV)患者涎液化糖链蛋白6(krebs von den lungen 6,KL-6)及氧化应激的影响。方法选取2016年1月-2018年4月入住重庆市巴南区人民医院并择期开胸手术行OLV患者76例纳入研究,随机分为生理盐水组(C组)和瑞芬太尼组(R组),每组38例。两组患者在OLV前1min(T1),OLV结束后5min(T2)及OLV结束35min(T3)分别采取颈内静脉血测定血清KL-6及氧化应激因子水平。结果两组在T2、T3时点血清KL-6均较本组T1时点显著升高,且T2、T3时点R组明显低于C组,差异有统计学意义(P<O.05)。C组血清丙二醛(maleic dialdehyde,MDA)T2、T3时点,R组T3时点较本组T1时点升高(P<O.05),且R组T2、T3时点较C组同时点低,差异有统计学意义(P<0.05)。C组血清超氧化物歧化酶(superoxide dismutase,SOD)在T2、T3时点,R组在T3时点较本组T1时点显著降低,且R组T2、T3时点SOD较C组同时点高,差异有统计学意义(P<0.05)。结论瑞芬太尼可改善OLV患者氧化应激状态,降低KL-6表达起到肺保护作用。展开更多
目的探讨高浓度氧通气对全身麻醉手术患者血清涎液化糖链蛋白6(krebs von den lungen 6,KL-6)及细胞因子表达的影响。方法经医院伦理学会批准,选取重庆市巴南区人民医院2018年1月2018年6月行全身麻醉手术者96例,按照随机数字表法分为A组...目的探讨高浓度氧通气对全身麻醉手术患者血清涎液化糖链蛋白6(krebs von den lungen 6,KL-6)及细胞因子表达的影响。方法经医院伦理学会批准,选取重庆市巴南区人民医院2018年1月2018年6月行全身麻醉手术者96例,按照随机数字表法分为A组(100%氧吸入)和B组(60%氧吸入),每组48例。分别于全身麻醉前30 min(T0)、全身麻醉后60 min(T1)、全身麻醉后120 min(T2)、全身麻醉后180 min(T3)、机械通气结束后60 min(T4)抽取桡动脉血行动脉血气分析。并于T0、T1、T2.T3及T4时间点抽取中心静脉血,检测血清KL-6、肿瘤坏死因子-a(TNF-x)、白细胞介素-8(IL-8)水平。结果两组患者全身麻醉前血清KL-6.TNF-α、IL-8组间比较差异无统计学意义(P>0.05)。全身麻醉后血清KL-6、TNF-α、IL-8均逐渐上升,其中A组T1血清KL-6、TNF-α、IL-8即显著高于T0[KL-6(U/mL):173.22±31.19比101.13±22.31;TNF-a(pg/mL):5.99±2.81比3.24±1.98;IL-8(pg/mL):128.11±32.21比98.22±19.77,均P<0.05]。B组T1血清KL-6高于T0[KL-6(U/mL):121.64±33.55比99.29±24.93]。T2血清TNF-α、IL-8高于TO0[TNF-a(pg/mL):4.41±2.71比3.24±1.98;IL-8(pg/mL):123.21±31.47比98.22±19.77,均P<0.05]。A组T1-T4血清KL-6、TNF-α.IL-8分别为[KL-6:(173.22±31.19)(189.38±39.11)(236.16±49.37)(210.04±38.66)U/mL;TNF-a(5.99±2.81)(6.21±2.56)(6.92±3.01)(6.31±2.88)pg/mL;IL-8:(128.11±32.21)(155.17±42.19)(188.28±40.33)(171.15±43.11)pg/mL],均高于同时点B组[KL-6:(121.64±33.55>/(141.33±36.74)(168.04±42.55)(166.39±44.63)U/mL;TNF-α(3.31±2.11)(4.41±2.71)(4.50±3.12)(4.39±2.41)pg/mL;IL-8:(103.12±23.3(123.21±31.47)(135.194.33)(137.339.22)pg/mL],差异均有统计学意义(均P<0.05)。结论全身麻醉术中高浓度氧气吸入可引起患者血清细胞因子及KL-6表达增多。展开更多
Formic acid silages were prepared from fresh and cooked sprat and squid viscera.Two types of silage were stored at 6,13,20,25,30 and 39℃ for 32 days.Raw material and silage in fresh and after 1,2,3,4,8,16 and 32 days...Formic acid silages were prepared from fresh and cooked sprat and squid viscera.Two types of silage were stored at 6,13,20,25,30 and 39℃ for 32 days.Raw material and silage in fresh and after 1,2,3,4,8,16 and 32 days of storage were analyzed.The dry matter,protein,fat and ash in silage reflected the composition of the raw material and were not affected by the different storage temperatures and times.Neither was there any change in amino acid contents.The degree of hydrolysis varied with the temperatures under which the silages were stored and were low in silage than in their aquatic phase respectively.The silages made from cooked raw material showed no changes in hydrolysis during storage.Five diets,in which the including levels of squid offal silage for replacing fish meal as protein resource were 0,5%,10%,20%,30% respectively,were formulated for puffer and flounder growth experiments.The results showed that exchanging part of dietary fish meal with silage improved the growth performance of fish.展开更多
文摘目的研究瑞芬太尼对开胸手术单肺通气(one lung ventilation,OLV)患者涎液化糖链蛋白6(krebs von den lungen 6,KL-6)及氧化应激的影响。方法选取2016年1月-2018年4月入住重庆市巴南区人民医院并择期开胸手术行OLV患者76例纳入研究,随机分为生理盐水组(C组)和瑞芬太尼组(R组),每组38例。两组患者在OLV前1min(T1),OLV结束后5min(T2)及OLV结束35min(T3)分别采取颈内静脉血测定血清KL-6及氧化应激因子水平。结果两组在T2、T3时点血清KL-6均较本组T1时点显著升高,且T2、T3时点R组明显低于C组,差异有统计学意义(P<O.05)。C组血清丙二醛(maleic dialdehyde,MDA)T2、T3时点,R组T3时点较本组T1时点升高(P<O.05),且R组T2、T3时点较C组同时点低,差异有统计学意义(P<0.05)。C组血清超氧化物歧化酶(superoxide dismutase,SOD)在T2、T3时点,R组在T3时点较本组T1时点显著降低,且R组T2、T3时点SOD较C组同时点高,差异有统计学意义(P<0.05)。结论瑞芬太尼可改善OLV患者氧化应激状态,降低KL-6表达起到肺保护作用。
文摘目的探讨高浓度氧通气对全身麻醉手术患者血清涎液化糖链蛋白6(krebs von den lungen 6,KL-6)及细胞因子表达的影响。方法经医院伦理学会批准,选取重庆市巴南区人民医院2018年1月2018年6月行全身麻醉手术者96例,按照随机数字表法分为A组(100%氧吸入)和B组(60%氧吸入),每组48例。分别于全身麻醉前30 min(T0)、全身麻醉后60 min(T1)、全身麻醉后120 min(T2)、全身麻醉后180 min(T3)、机械通气结束后60 min(T4)抽取桡动脉血行动脉血气分析。并于T0、T1、T2.T3及T4时间点抽取中心静脉血,检测血清KL-6、肿瘤坏死因子-a(TNF-x)、白细胞介素-8(IL-8)水平。结果两组患者全身麻醉前血清KL-6.TNF-α、IL-8组间比较差异无统计学意义(P>0.05)。全身麻醉后血清KL-6、TNF-α、IL-8均逐渐上升,其中A组T1血清KL-6、TNF-α、IL-8即显著高于T0[KL-6(U/mL):173.22±31.19比101.13±22.31;TNF-a(pg/mL):5.99±2.81比3.24±1.98;IL-8(pg/mL):128.11±32.21比98.22±19.77,均P<0.05]。B组T1血清KL-6高于T0[KL-6(U/mL):121.64±33.55比99.29±24.93]。T2血清TNF-α、IL-8高于TO0[TNF-a(pg/mL):4.41±2.71比3.24±1.98;IL-8(pg/mL):123.21±31.47比98.22±19.77,均P<0.05]。A组T1-T4血清KL-6、TNF-α.IL-8分别为[KL-6:(173.22±31.19)(189.38±39.11)(236.16±49.37)(210.04±38.66)U/mL;TNF-a(5.99±2.81)(6.21±2.56)(6.92±3.01)(6.31±2.88)pg/mL;IL-8:(128.11±32.21)(155.17±42.19)(188.28±40.33)(171.15±43.11)pg/mL],均高于同时点B组[KL-6:(121.64±33.55>/(141.33±36.74)(168.04±42.55)(166.39±44.63)U/mL;TNF-α(3.31±2.11)(4.41±2.71)(4.50±3.12)(4.39±2.41)pg/mL;IL-8:(103.12±23.3(123.21±31.47)(135.194.33)(137.339.22)pg/mL],差异均有统计学意义(均P<0.05)。结论全身麻醉术中高浓度氧气吸入可引起患者血清细胞因子及KL-6表达增多。
文摘Formic acid silages were prepared from fresh and cooked sprat and squid viscera.Two types of silage were stored at 6,13,20,25,30 and 39℃ for 32 days.Raw material and silage in fresh and after 1,2,3,4,8,16 and 32 days of storage were analyzed.The dry matter,protein,fat and ash in silage reflected the composition of the raw material and were not affected by the different storage temperatures and times.Neither was there any change in amino acid contents.The degree of hydrolysis varied with the temperatures under which the silages were stored and were low in silage than in their aquatic phase respectively.The silages made from cooked raw material showed no changes in hydrolysis during storage.Five diets,in which the including levels of squid offal silage for replacing fish meal as protein resource were 0,5%,10%,20%,30% respectively,were formulated for puffer and flounder growth experiments.The results showed that exchanging part of dietary fish meal with silage improved the growth performance of fish.
