[ Objective ] The aim of the research was to reveal the mechanism of Yizhikang powder treatment on dairy cattle with retained placenta from the hemorheological perspective. [ Method] Dairy cattle with retained placent...[ Objective ] The aim of the research was to reveal the mechanism of Yizhikang powder treatment on dairy cattle with retained placenta from the hemorheological perspective. [ Method] Dairy cattle with retained placenta were treated with oral administration of Yizhikang powder. And their hemorheological indexes were measured and compared with the corresponding indicators of pre-administration group, healthy group, and control group （sick but untreated）.[ Result] There was large decrease amplitude in the indexes （whole blood viscosity, plasma viscosity, whole blood viscosity reduction viscosity, ESR, ESR equation K value, fibrinogen content, platelet aggregation rate, RBC deformability IF value） of treated dairy cattle with retained placenta. By t test, the indexes, except hematocrit, decreased significantly after treatment （P〈0.05） and reached the status of post partum healthy cows. The hemorheological indexes didn＇t change significantly in the control group before and after treatment. [ Conclusion] Yizhikang powder could significantly improve blood flow state and reduced the occurrence of qi stagnation and blood stasis in perinatal period.展开更多
The electrical safety requirements of soybean milk machines with Chinese food cooking characteristics and thefunctions of liquid heating and material crushing cannot be simply constituted by combining liquid heater st...The electrical safety requirements of soybean milk machines with Chinese food cooking characteristics and thefunctions of liquid heating and material crushing cannot be simply constituted by combining liquid heater standard （IEC 60335-2-15） and kitchen appliances standard （IEC 60335-2-14）. The alternating operation of rotation and heating and the antiburnt function may be the special features of safety and performance of soybean milk machines, so it is necessary to take into account those special features in the current standards by some means.展开更多
Here we propose a method for determination of Avermectin residual content in milk and beef content. After extracting and processing the sample, use high performance liquid chromatography （HPLC） for determination. Mo...Here we propose a method for determination of Avermectin residual content in milk and beef content. After extracting and processing the sample, use high performance liquid chromatography （HPLC） for determination. Mobile phase is： V（Methanol）： V（Water）=90： 10; determination wavelength is 245 nm; flow rate is 0.8 mL/min. Avermectin is completely separated in 17min, linear range is 5 - 0.3 g/L, correlation coefficient is 0.9997, average recovery rates of Avermectin in milk and beef are respectively 91.20 %- 100.32 % and 89.93 %-99.15 %, the relative standard deviations are respectively less than 7% and 4%. This method is simple, sensitive and accurate, so is suitable for determination of Avermectin residues in milk and beef, and it can meet domestic and foreign testing requirements.展开更多
Heat stress affects feed intake, milk production, and endocrine status in dairy cows. The temperaturehumidity index（THI） is employed as an index to evaluate the degree of heat stress in dairy cows. However, it is di...Heat stress affects feed intake, milk production, and endocrine status in dairy cows. The temperaturehumidity index（THI） is employed as an index to evaluate the degree of heat stress in dairy cows. However, it is difficult to ascertain whether THI is the most appropriate measurement of heat stress in dairy cows. This experiment was conducted to investigate the effects of heat stress on serum insulin, adipokines（leptin and adiponectin）, AMP-activated protein kinase（AMPK）, and heat shock signal molecules（heat shock transcription factor（HSF） and heat shock proteins（HSP）） in dairy cows and to research biomarkers to be used for better understanding the meaning of THI as a bioclimatic index. To achieve these objectives, two experiments were performed. The first experiment： eighteen lactating Holstein dairy cows were used. The treatments were： heat stress（HS, THI average=81.7, n=9） and cooling（CL, THI average=53.4, n=9）. Samples of HS were obtained on August 16, 2013, and samples of CL were collected on April 7, 2014 in natural conditions. The second experiment： HS treatment cows（n=9） from the first experiment were fed for 8 weeks from August 16, 2013 to October 12, 2013. Samples for moderate heat stress, mild heat stress, and no heat stress were obtained, respectively, according to the physical alterations of the THI. Results showed that heat stress significantly increased the serum adiponectin, AMPK, HSF, HSP27, HSP70, and HSP90（P〈0.05）. Adiponectin is strongly associated with AMPK. The increases of adiponectin and AMPK may be one of the mechanisms to maintain homeostasis in heat-stressed dairy cows. When heat stress treatment lasted 8 weeks, a higher expression of HSF and HSP70 was observed under moderate heat stress. Serum HSF and HSP70 are sensitive and accurate in heat stress and they could be potential indicators of animal response to heat stress. We recommend serum HSF and HSP70 as meaningful biomarkers to supplement the THI and evaluate moderate heat stress in dairy cows in the future.展开更多
基金Supported by Science and Technology Key Projects of Xinjiang Pro-duction and Construction Corps (2006GG22)~~
文摘[ Objective ] The aim of the research was to reveal the mechanism of Yizhikang powder treatment on dairy cattle with retained placenta from the hemorheological perspective. [ Method] Dairy cattle with retained placenta were treated with oral administration of Yizhikang powder. And their hemorheological indexes were measured and compared with the corresponding indicators of pre-administration group, healthy group, and control group （sick but untreated）.[ Result] There was large decrease amplitude in the indexes （whole blood viscosity, plasma viscosity, whole blood viscosity reduction viscosity, ESR, ESR equation K value, fibrinogen content, platelet aggregation rate, RBC deformability IF value） of treated dairy cattle with retained placenta. By t test, the indexes, except hematocrit, decreased significantly after treatment （P〈0.05） and reached the status of post partum healthy cows. The hemorheological indexes didn＇t change significantly in the control group before and after treatment. [ Conclusion] Yizhikang powder could significantly improve blood flow state and reduced the occurrence of qi stagnation and blood stasis in perinatal period.
文摘The electrical safety requirements of soybean milk machines with Chinese food cooking characteristics and thefunctions of liquid heating and material crushing cannot be simply constituted by combining liquid heater standard （IEC 60335-2-15） and kitchen appliances standard （IEC 60335-2-14）. The alternating operation of rotation and heating and the antiburnt function may be the special features of safety and performance of soybean milk machines, so it is necessary to take into account those special features in the current standards by some means.
文摘Here we propose a method for determination of Avermectin residual content in milk and beef content. After extracting and processing the sample, use high performance liquid chromatography （HPLC） for determination. Mobile phase is： V（Methanol）： V（Water）=90： 10; determination wavelength is 245 nm; flow rate is 0.8 mL/min. Avermectin is completely separated in 17min, linear range is 5 - 0.3 g/L, correlation coefficient is 0.9997, average recovery rates of Avermectin in milk and beef are respectively 91.20 %- 100.32 % and 89.93 %-99.15 %, the relative standard deviations are respectively less than 7% and 4%. This method is simple, sensitive and accurate, so is suitable for determination of Avermectin residues in milk and beef, and it can meet domestic and foreign testing requirements.
基金Project supported by the National Basic Research Program(973)of China(No.2011CB100805)the Modern Agro-Industry Technology Research System of China(No.nycytx-04-01)the Agricultural Science and Technology Innovation Program(No.ASTIP-IAS12),China
文摘Heat stress affects feed intake, milk production, and endocrine status in dairy cows. The temperaturehumidity index（THI） is employed as an index to evaluate the degree of heat stress in dairy cows. However, it is difficult to ascertain whether THI is the most appropriate measurement of heat stress in dairy cows. This experiment was conducted to investigate the effects of heat stress on serum insulin, adipokines（leptin and adiponectin）, AMP-activated protein kinase（AMPK）, and heat shock signal molecules（heat shock transcription factor（HSF） and heat shock proteins（HSP）） in dairy cows and to research biomarkers to be used for better understanding the meaning of THI as a bioclimatic index. To achieve these objectives, two experiments were performed. The first experiment： eighteen lactating Holstein dairy cows were used. The treatments were： heat stress（HS, THI average=81.7, n=9） and cooling（CL, THI average=53.4, n=9）. Samples of HS were obtained on August 16, 2013, and samples of CL were collected on April 7, 2014 in natural conditions. The second experiment： HS treatment cows（n=9） from the first experiment were fed for 8 weeks from August 16, 2013 to October 12, 2013. Samples for moderate heat stress, mild heat stress, and no heat stress were obtained, respectively, according to the physical alterations of the THI. Results showed that heat stress significantly increased the serum adiponectin, AMPK, HSF, HSP27, HSP70, and HSP90（P〈0.05）. Adiponectin is strongly associated with AMPK. The increases of adiponectin and AMPK may be one of the mechanisms to maintain homeostasis in heat-stressed dairy cows. When heat stress treatment lasted 8 weeks, a higher expression of HSF and HSP70 was observed under moderate heat stress. Serum HSF and HSP70 are sensitive and accurate in heat stress and they could be potential indicators of animal response to heat stress. We recommend serum HSF and HSP70 as meaningful biomarkers to supplement the THI and evaluate moderate heat stress in dairy cows in the future.
