LCM （life cycle management） is a systematic approach, mindset and culture that considers economic, social, and environmental factors among other factors in the decision making process throughout various business or ...LCM （life cycle management） is a systematic approach, mindset and culture that considers economic, social, and environmental factors among other factors in the decision making process throughout various business or organizational decisions that affect both inputs and outputs of a product or service life cycle. It is a product, process, or activity management system aimed at minimizing environmental and socio-economic burdens associated with an organization＇s product or process during its entire life cycle and value chain. LCM＇s application is gaining wider acceptance both in the corporate and governmental organizations as an approach to reduce ecological footprints and to improve the sustainability of human activities. But where and how can it be used in agricultural engineering applications？ This study highlights the potential areas of LCM application in agricultural and allied sectors and how it can be utilized. The study revealed that LCM tools such as design for environment and life cycle analysis can be used to evaluate the environmental impacts of-and to improve the products, equipment, and structures produced by biosystems engineers as well as the processes used to generate them.展开更多
AIM： To develop a method of labeling and microdissecting mouse Kupffer cells within an extraordinarily short period of time using laser capture microdissection （LCM）. METHODS： Tissues are complex structures compri...AIM： To develop a method of labeling and microdissecting mouse Kupffer cells within an extraordinarily short period of time using laser capture microdissection （LCM）. METHODS： Tissues are complex structures comprised of a heterogeneous population of interconnected cells. LCM offers a method of isolating a single cell type from specific regions of a tissue section. LCM is an essential approach used in conjunction with molecular analysis to study the functional interaction of cells in their native tissue environment. The process of labeling and acquiring cells by LCM prior to mRNA isolation can be elaborate, thereby subjecting the RNA to considerable degradation. Kupffer cell labeling is achieved by injecting India ink intravenously, thus circumventing the need for in vitro staining. The significance of this novel approach was validated using a cholestatic liver injury model. RESULTS： mRNA extracted from the microdissected cell population displayed marked increases in colonystimulating factor-1 receptor and Kupffer cell receptor message expression, which demonstrated Kupffer cell enrichment. Gene expression by Kupffer ceils derived from bile-duct-ligated, versus sham-operated, mice was compared. Microarray analysis revealed a significant （2.5-fold, q value 〈 10） change in 493 genes. Based on this fold-change and a standardized PubMed search, 10 genes were identified that were relevant to the ability of Kupffer cells to suppress liver injury. CONCLUSION; The methodology outlined herein provides an approach to isolating high quality RNA from Kupffer cells, without altering the tissue integrity.展开更多
文摘LCM （life cycle management） is a systematic approach, mindset and culture that considers economic, social, and environmental factors among other factors in the decision making process throughout various business or organizational decisions that affect both inputs and outputs of a product or service life cycle. It is a product, process, or activity management system aimed at minimizing environmental and socio-economic burdens associated with an organization＇s product or process during its entire life cycle and value chain. LCM＇s application is gaining wider acceptance both in the corporate and governmental organizations as an approach to reduce ecological footprints and to improve the sustainability of human activities. But where and how can it be used in agricultural engineering applications？ This study highlights the potential areas of LCM application in agricultural and allied sectors and how it can be utilized. The study revealed that LCM tools such as design for environment and life cycle analysis can be used to evaluate the environmental impacts of-and to improve the products, equipment, and structures produced by biosystems engineers as well as the processes used to generate them.
基金Supported by NIH Grant DK068097funds provided by Rhode Island Hospital+1 种基金the Deutsche Forschungsgemeinschaft grant (DFG) grant GE1193/1-1NIH COBRE Award (RR-P20 RR17695)
文摘AIM： To develop a method of labeling and microdissecting mouse Kupffer cells within an extraordinarily short period of time using laser capture microdissection （LCM）. METHODS： Tissues are complex structures comprised of a heterogeneous population of interconnected cells. LCM offers a method of isolating a single cell type from specific regions of a tissue section. LCM is an essential approach used in conjunction with molecular analysis to study the functional interaction of cells in their native tissue environment. The process of labeling and acquiring cells by LCM prior to mRNA isolation can be elaborate, thereby subjecting the RNA to considerable degradation. Kupffer cell labeling is achieved by injecting India ink intravenously, thus circumventing the need for in vitro staining. The significance of this novel approach was validated using a cholestatic liver injury model. RESULTS： mRNA extracted from the microdissected cell population displayed marked increases in colonystimulating factor-1 receptor and Kupffer cell receptor message expression, which demonstrated Kupffer cell enrichment. Gene expression by Kupffer ceils derived from bile-duct-ligated, versus sham-operated, mice was compared. Microarray analysis revealed a significant （2.5-fold, q value 〈 10） change in 493 genes. Based on this fold-change and a standardized PubMed search, 10 genes were identified that were relevant to the ability of Kupffer cells to suppress liver injury. CONCLUSION; The methodology outlined herein provides an approach to isolating high quality RNA from Kupffer cells, without altering the tissue integrity.
