目的:分析成人社区获得性肺炎(community-acquired pneumonia, CAP)患者严重程度与乳酸脱氢酶(lactate dehydrogenase, LDH)、血清淀粉样蛋白A(serum amyloid protein A, SAA)的相关性。方法:选择2017年11月至2018年10月就诊于我院的CA...目的:分析成人社区获得性肺炎(community-acquired pneumonia, CAP)患者严重程度与乳酸脱氢酶(lactate dehydrogenase, LDH)、血清淀粉样蛋白A(serum amyloid protein A, SAA)的相关性。方法:选择2017年11月至2018年10月就诊于我院的CAP患者100例,记录患者性别、年龄、个人病史、体质指数(body mass index, BMI),测量血压、心率、每分钟呼吸次数,评估意识状态,完善胸部CT、血气分析,进行血常规、尿素氮、降钙素原(procalcitonin, PCT)、C反应蛋白(C reactive protein, CRP)、LDH、SAA检查。根据病情将患者分为普通肺炎组(n=62)、重症肺炎组(n=38),比较两组患者各项指标差异,分析各项指标与肺炎严重程度的关系。结果:普通肺炎组白细胞(WBC)、PCT、CRP、LDH、SAA水平低于重症肺炎组(P<0.05)。Logistic回归分析提示,LDH、SAA与患者病情严重程度相关(P<0.05);受试者工作特征(receiver operating characteristic, ROC)曲线分析提示LDH的曲线下面积(area under the curve, AUC)为0.894,截点为228.5 U/L,敏感度为81.6%,特异度为87.1%,Youden指数为0.687[95%置信区间(CI)0.819～0.969,P=0.000];SAA的AUC为0.802,截点为170 mg/L,敏感度为84.2%,特异度为85.5%,Youden指数为0.697(95%CI 0.714～0.889,P=0.000)。结论:LDH、SAA与CAP患者病情严重程度相关,可反映患者病情的严重性,有利于患者病情的评估。展开更多
The bifunctional fusion protein systems consisting of Rhodococcus erythropolis chiral alcohol dehydrogenase(READH),Candida boidinii formate dehydrogenase(CbFDH) or maltose binding protein(MBP) were constructed to rege...The bifunctional fusion protein systems consisting of Rhodococcus erythropolis chiral alcohol dehydrogenase(READH),Candida boidinii formate dehydrogenase(CbFDH) or maltose binding protein(MBP) were constructed to regenerate the cofactors for biocatalysis.READH originated from Rhodococcus erythropolis is an(S)-specific nicotinamide adenine dinucleotide(NADH)-dependent alcohol dehydrogenase,meanwhile,CbFDH originated from Candida boidinii is an NADH-dependent formate dehydrogenase.The strategies of the different fusion protein systems included:(1) fusion of the N terminus of READH to the C terminus of MBP,(2) fusion of the N terminus of CbFDH to the C terminus of MBP,(3) fusion of the N terminus of READH to the C terminus of CbFDH,(4) fusion of the C terminus of READH to the N terminus of CbFDH.The activities of READHs were depressed in all fusion strategies.When the N terminus of READH was fused to the C terminus of CbFDH,READH reached the highest activity,but CbFDH had no activity.In contrast,when the C terminus of READH was fused to the N terminus of CbFDH,CbFDH showed the highest activity,and both moieties displayed activities.From this study,the authors suggest that the rational design of the bifunctional fusion protein system may improve the biocatalysis efficiency by the simultaneous cofactor regeneration.展开更多
文摘The bifunctional fusion protein systems consisting of Rhodococcus erythropolis chiral alcohol dehydrogenase(READH),Candida boidinii formate dehydrogenase(CbFDH) or maltose binding protein(MBP) were constructed to regenerate the cofactors for biocatalysis.READH originated from Rhodococcus erythropolis is an(S)-specific nicotinamide adenine dinucleotide(NADH)-dependent alcohol dehydrogenase,meanwhile,CbFDH originated from Candida boidinii is an NADH-dependent formate dehydrogenase.The strategies of the different fusion protein systems included:(1) fusion of the N terminus of READH to the C terminus of MBP,(2) fusion of the N terminus of CbFDH to the C terminus of MBP,(3) fusion of the N terminus of READH to the C terminus of CbFDH,(4) fusion of the C terminus of READH to the N terminus of CbFDH.The activities of READHs were depressed in all fusion strategies.When the N terminus of READH was fused to the C terminus of CbFDH,READH reached the highest activity,but CbFDH had no activity.In contrast,when the C terminus of READH was fused to the N terminus of CbFDH,CbFDH showed the highest activity,and both moieties displayed activities.From this study,the authors suggest that the rational design of the bifunctional fusion protein system may improve the biocatalysis efficiency by the simultaneous cofactor regeneration.
