英国酿造企业投资创立的研究协会 Brewing Research Foundation 宣布,93年1月向英国政府申请利用重组啤酒酵母进行啤酒工业化试验。该协会理事 B.Atkison 说:"打算通过这项实验证明基因操作技术在啤酒酿造中已是平常的技术。"...英国酿造企业投资创立的研究协会 Brewing Research Foundation 宣布,93年1月向英国政府申请利用重组啤酒酵母进行啤酒工业化试验。该协会理事 B.Atkison 说:"打算通过这项实验证明基因操作技术在啤酒酿造中已是平常的技术。"目前这项实验如能得到批准,将是世界首家利用微生物工业化规模生产发酵食品。实际上,实验概要是在啤酒酵母中导入来自其他酿造用酵母的淀粉葡糖苷酶基因,酿造碳氢化合物残留量少的优质啤酒。作为食品用酶正在英国销售淀粉葡糖苷酶。培养规模最大10万升(工业规模)。安全度为 GILSP。展开更多
AIM: To investigate the effect of Bu-Zhong-Yi-Qi-Tang (Decoction for Reinforcing Middle Jiao and Replenishing Qi) on deficiency of N-glycan/nitric oxide (NO) and islet damage induced by injecting two medium doses of s...AIM: To investigate the effect of Bu-Zhong-Yi-Qi-Tang (Decoction for Reinforcing Middle Jiao and Replenishing Qi) on deficiency of N-glycan/nitric oxide (NO) and islet damage induced by injecting two medium doses of streptozotocin (STZ). METHODS: Diabetes was induced by intraperitoneal injection of STZ at 55 mg/kg on day 1 and day 8. Islet damage was evaluated using a scoring system. Nitrite, nitrate, α-mannosidase and amylase activities were measured by colorimetry. N-glycan patterns of amylase were determined with lectin [ConA, pisum sativum agglutinin (PSA), peanut agglutinin (PNA), and lens culinaris agglutinin (LCA)] affinity precipitation method. RESULTS: Severe islet necrosis and mild islet atrophy were observed in diabetic rats. The number and size ofislets, the activities of α-mannosidase, amylase and nitrite were decreased, while the binding of PNA and LCA to amylase was increased. All of which were improved after treatment with Bu-Zhong-Yi-Qi-Tang. Islet damage was significantly correlated with nitrite, nitrate, α-mannosidase, amylase and the binding of LCA, PNA, and PSA to amylase.CONCLUSION: STZ-induced islet damage is related to N-glycan deficiency in proteins by blocking α-mannosidase activity and no deficiency, accumulation of unfolded proteins, and endoplasmic reticulum stress and activation of cellular signals, all of which are improved after treatment with Bu-Zhong-YiQi-Tang.展开更多
The chemical composition and biological activity of Sanghuangporus vaninii solid culture treated by static magnetic field were studied.The compounds were isolated and purified using ultrasonic extraction and semi-prep...The chemical composition and biological activity of Sanghuangporus vaninii solid culture treated by static magnetic field were studied.The compounds were isolated and purified using ultrasonic extraction and semi-preparative liquid chromatography.Their structures were identified through spectral data,and the inhibitory activities againstα-amylase,α-glucosidase and pancreatic lipase were evaluated.Three compounds,5,7-dihydroxy-3,4'-dimethoxyflavone(1),D-(+)-Trehalose(2),pinolenic acid(3),were extracted from the petroleum ether layer.Comparison of peak heights indicated a significantly higher content of compounds subjected to a 4 mT static magnetic field compared to those untreated.Activity tests revealed that compounds 1 and 2 exhibited strong anti-α-amylase and anti-α-glucosidase activities.Specifically,compound 1 had inhibition rates of(65.37±0.05)%and(73.81±0.12)%after 4 mT treatment,compared to(57.26±0.11)%and(65.33±0.14)%without.Compound 2 showed inhibition rates of(68.61±0.12)%and(65.38±0.09)%with the magnetic field,versus(60.71±0.06)%and(56.18±0.02)%without.Compound 3 displayed a notable inhibitory effect on pancreatic lipase,with rates of(60.83±0.03)%after 4 mT treatment and(53.77±0.09)%without.This study demonstrates that the static magnetic field enhances the chemical content and bioactivity of Sanghuangporus vaninii solid culture,providing a basis for its further development and application.展开更多
文摘英国酿造企业投资创立的研究协会 Brewing Research Foundation 宣布,93年1月向英国政府申请利用重组啤酒酵母进行啤酒工业化试验。该协会理事 B.Atkison 说:"打算通过这项实验证明基因操作技术在啤酒酿造中已是平常的技术。"目前这项实验如能得到批准,将是世界首家利用微生物工业化规模生产发酵食品。实际上,实验概要是在啤酒酵母中导入来自其他酿造用酵母的淀粉葡糖苷酶基因,酿造碳氢化合物残留量少的优质啤酒。作为食品用酶正在英国销售淀粉葡糖苷酶。培养规模最大10万升(工业规模)。安全度为 GILSP。
基金Supported by The Project of Guangdong Science and Technology
文摘AIM: To investigate the effect of Bu-Zhong-Yi-Qi-Tang (Decoction for Reinforcing Middle Jiao and Replenishing Qi) on deficiency of N-glycan/nitric oxide (NO) and islet damage induced by injecting two medium doses of streptozotocin (STZ). METHODS: Diabetes was induced by intraperitoneal injection of STZ at 55 mg/kg on day 1 and day 8. Islet damage was evaluated using a scoring system. Nitrite, nitrate, α-mannosidase and amylase activities were measured by colorimetry. N-glycan patterns of amylase were determined with lectin [ConA, pisum sativum agglutinin (PSA), peanut agglutinin (PNA), and lens culinaris agglutinin (LCA)] affinity precipitation method. RESULTS: Severe islet necrosis and mild islet atrophy were observed in diabetic rats. The number and size ofislets, the activities of α-mannosidase, amylase and nitrite were decreased, while the binding of PNA and LCA to amylase was increased. All of which were improved after treatment with Bu-Zhong-Yi-Qi-Tang. Islet damage was significantly correlated with nitrite, nitrate, α-mannosidase, amylase and the binding of LCA, PNA, and PSA to amylase.CONCLUSION: STZ-induced islet damage is related to N-glycan deficiency in proteins by blocking α-mannosidase activity and no deficiency, accumulation of unfolded proteins, and endoplasmic reticulum stress and activation of cellular signals, all of which are improved after treatment with Bu-Zhong-YiQi-Tang.
基金supported by the Natural Science Foundation of Jilin Province(20220402050 GH,JJKH20220826KJ)Natural Science Foundation of Changchun Normal University(2020004).
文摘The chemical composition and biological activity of Sanghuangporus vaninii solid culture treated by static magnetic field were studied.The compounds were isolated and purified using ultrasonic extraction and semi-preparative liquid chromatography.Their structures were identified through spectral data,and the inhibitory activities againstα-amylase,α-glucosidase and pancreatic lipase were evaluated.Three compounds,5,7-dihydroxy-3,4'-dimethoxyflavone(1),D-(+)-Trehalose(2),pinolenic acid(3),were extracted from the petroleum ether layer.Comparison of peak heights indicated a significantly higher content of compounds subjected to a 4 mT static magnetic field compared to those untreated.Activity tests revealed that compounds 1 and 2 exhibited strong anti-α-amylase and anti-α-glucosidase activities.Specifically,compound 1 had inhibition rates of(65.37±0.05)%and(73.81±0.12)%after 4 mT treatment,compared to(57.26±0.11)%and(65.33±0.14)%without.Compound 2 showed inhibition rates of(68.61±0.12)%and(65.38±0.09)%with the magnetic field,versus(60.71±0.06)%and(56.18±0.02)%without.Compound 3 displayed a notable inhibitory effect on pancreatic lipase,with rates of(60.83±0.03)%after 4 mT treatment and(53.77±0.09)%without.This study demonstrates that the static magnetic field enhances the chemical content and bioactivity of Sanghuangporus vaninii solid culture,providing a basis for its further development and application.
