To investigate the imbalance state of helper T lymphocytes （Th） and cytotoxic T lymphocytes （Tc） and the roles of Thl/Th2/Th3 and Tcl/Tc2 cells in renal transplantation rejection, the percentages of these cells in...To investigate the imbalance state of helper T lymphocytes （Th） and cytotoxic T lymphocytes （Tc） and the roles of Thl/Th2/Th3 and Tcl/Tc2 cells in renal transplantation rejection, the percentages of these cells in peripheral blood of 24 cases of renal transplantation recipients with acute rejection and the dynamic changes of the CD4/CD8 ratio were determined by flow cytometry analysis, while 30 cases of healthy individuals were set up as controls. In these healthy controls, the percentages of the Thl, Th2 and Th3 cells were （ 10.45 ± 8.15） %, （5.05 ± 4.15） % and （3.90 ± 3.21 ） %, and those of Tcl and %2 cells were （9.83 ± 7.03） % and （4.51 ± 2.17） %, respectively. However, the percentages of Thl and Tcl cells in peripheral blood of the stable recipients after transplantation were （7.29 ± 5.62） % and （7.04 ± 5.15）%, showing definite reduction, while those of Th2, Th3 and Tc2 cells showed significant increase, （ 6.34 ± 5.67） %, （4.94 ± 4.14） % and （ 6.86 ± 4.42） %, respectively. In case of recipients with acute rejection, the percentages of Thl and Tcl cells appeared to be （ 18.55 ± 13.21 ） % and （ 15.84 ± 11.72） %, also showing significant increase, but those of Th2, Th3 and %2 cells appeared to be reduced, （4.19 ± 3.62） %, （3.02 ± 2.83 ） % and （3.88 ± 1.63） %, respectively. Significant differences could be detected among these three groups （ P 〈 0.05）. The CD4/CD8 ratio in cases with acute rejection was higher than those of stable recipients （2.24 ± 0.59 vs 1.95 ± 0.45）, but that of the stable recipients and healthy controls （ 1.98 ± 0.31 ） showed no any significant difference. From the above observation, it is evident that imbalance between Thl, Th2 and Th3 with Tcl and Tc2 cells may exist after renal transplantation and probably, the im- mune imbalance may be induced through the secretion of cytokines INF-γby Thl or Tcl cells , Ⅱ-4 by Th2 and Tc2 cells and TGF-β by Th3.展开更多
文摘To investigate the imbalance state of helper T lymphocytes （Th） and cytotoxic T lymphocytes （Tc） and the roles of Thl/Th2/Th3 and Tcl/Tc2 cells in renal transplantation rejection, the percentages of these cells in peripheral blood of 24 cases of renal transplantation recipients with acute rejection and the dynamic changes of the CD4/CD8 ratio were determined by flow cytometry analysis, while 30 cases of healthy individuals were set up as controls. In these healthy controls, the percentages of the Thl, Th2 and Th3 cells were （ 10.45 ± 8.15） %, （5.05 ± 4.15） % and （3.90 ± 3.21 ） %, and those of Tcl and %2 cells were （9.83 ± 7.03） % and （4.51 ± 2.17） %, respectively. However, the percentages of Thl and Tcl cells in peripheral blood of the stable recipients after transplantation were （7.29 ± 5.62） % and （7.04 ± 5.15）%, showing definite reduction, while those of Th2, Th3 and Tc2 cells showed significant increase, （ 6.34 ± 5.67） %, （4.94 ± 4.14） % and （ 6.86 ± 4.42） %, respectively. In case of recipients with acute rejection, the percentages of Thl and Tcl cells appeared to be （ 18.55 ± 13.21 ） % and （ 15.84 ± 11.72） %, also showing significant increase, but those of Th2, Th3 and %2 cells appeared to be reduced, （4.19 ± 3.62） %, （3.02 ± 2.83 ） % and （3.88 ± 1.63） %, respectively. Significant differences could be detected among these three groups （ P 〈 0.05）. The CD4/CD8 ratio in cases with acute rejection was higher than those of stable recipients （2.24 ± 0.59 vs 1.95 ± 0.45）, but that of the stable recipients and healthy controls （ 1.98 ± 0.31 ） showed no any significant difference. From the above observation, it is evident that imbalance between Thl, Th2 and Th3 with Tcl and Tc2 cells may exist after renal transplantation and probably, the im- mune imbalance may be induced through the secretion of cytokines INF-γby Thl or Tcl cells , Ⅱ-4 by Th2 and Tc2 cells and TGF-β by Th3.
