本文检验了40例肾虚病人外周血 NK 细胞活性以及单个核细胞产生 IL—2和 IL—2受体表达能力,结果:肾虚病人 NK 活性、IL—2和 IL—2受体活性均较正常对照显著降低(P<0.01);正常对照 NK 活性与 IL—2活性呈直线正相关,而肾虚病人无明...本文检验了40例肾虚病人外周血 NK 细胞活性以及单个核细胞产生 IL—2和 IL—2受体表达能力,结果:肾虚病人 NK 活性、IL—2和 IL—2受体活性均较正常对照显著降低(P<0.01);正常对照 NK 活性与 IL—2活性呈直线正相关,而肾虚病人无明显相关性。展开更多
Optimum conditions for in vitro production of interleukin 2 （IL 2） like activity from the Peripheral blood lymphocytes （PBL） of an Indian major carp, Labeo rohita were studied. Culture supernatants were generated ...Optimum conditions for in vitro production of interleukin 2 （IL 2） like activity from the Peripheral blood lymphocytes （PBL） of an Indian major carp, Labeo rohita were studied. Culture supernatants were generated by culturing the PBL in RPMI-1640 media supplemented with Glutamine and 10% fetal bovine serum （FBS） and stimulating with two different mitogens： concanavalin A （Con A） and Phytohaemagglutinin （PHA） at different concentrations separately. Significantly （P 〈 0.01） higher proliferation response was obtained from the culture supematant stimulated with concanavalin A （Con A） at a concentration of 10 lag mLl. The effect of phorbol myristate acetate （PMA） was also studied by co-stimulating PBL with Con A and PHA separately and it was found to synergistically enhancing the stimulation index with Con A whereas the stimulation index remain unchanged with PHA. The Con A （10 μg mLl） stimulated PBL were also cultured at different cell density, incubation period and incubation temperature in order to optimize the in vitro L. rohita IL2 production. The IL2 like activity was studied by lymphocyte proliferation assay on 72 h Con A blasts using WST based assay technique. Significantly （P 〈 0.01） higher stimulation indices were obtained when the PBL were cultured at a cell density of 1 × 10^6 cells mL^-1 for 30-36 h at an incubation temperature of 30 ℃. The IL2 like activity was purified by DEAE-Sepharose anion exchange chromatography and recorded between 70-130 mM NaCI with peak activity at 110 Mm NaCI. The molecular weight of the factor responsible for IL2 like activity was found to be 15-17 KD.展开更多
Gnaphalium oxyphyllum DC is a medicinal plant whose common uses by Mexican people include the treatment of cancer. The toxicity of the aqueous and organic fractions as well as the aqueous decoction of G. oxyphyllum va...Gnaphalium oxyphyllum DC is a medicinal plant whose common uses by Mexican people include the treatment of cancer. The toxicity of the aqueous and organic fractions as well as the aqueous decoction of G. oxyphyllum var. natalie F. J. Espinosa were assessed on three human cancer cell lines as well as in blood cells in healthy human lymphocyte cultures. Cytotoxic activity was assessed by the Sulforhodamine B method on HeLa （human cervical carcinoma）, T47D （human breast carcinoma） and 22Rvl （human prostate carcinoma cancer）. Colchicine was used as positive control. The decoction was also tested on lymphocytes from healthy donors through the mitotic index as biomarker. We used whole blood for these cultures and estimated the effect of the extract on platelets, leukocytes and erythrocytes. The aqueous decoction was cytotoxic （EDs0 〈 20 μg/mL） on the three cancer cell lines. The mitotic index in the exposed lymphocyte cultures did not significantly differ from the control nor the blood counts showed any difference between the experimental and control cultures. These results prove that the toxic effect of the aqueous decoction of G. oxyphyllum var. natalie is specific for cancer cell lines.展开更多
文摘Optimum conditions for in vitro production of interleukin 2 （IL 2） like activity from the Peripheral blood lymphocytes （PBL） of an Indian major carp, Labeo rohita were studied. Culture supernatants were generated by culturing the PBL in RPMI-1640 media supplemented with Glutamine and 10% fetal bovine serum （FBS） and stimulating with two different mitogens： concanavalin A （Con A） and Phytohaemagglutinin （PHA） at different concentrations separately. Significantly （P 〈 0.01） higher proliferation response was obtained from the culture supematant stimulated with concanavalin A （Con A） at a concentration of 10 lag mLl. The effect of phorbol myristate acetate （PMA） was also studied by co-stimulating PBL with Con A and PHA separately and it was found to synergistically enhancing the stimulation index with Con A whereas the stimulation index remain unchanged with PHA. The Con A （10 μg mLl） stimulated PBL were also cultured at different cell density, incubation period and incubation temperature in order to optimize the in vitro L. rohita IL2 production. The IL2 like activity was studied by lymphocyte proliferation assay on 72 h Con A blasts using WST based assay technique. Significantly （P 〈 0.01） higher stimulation indices were obtained when the PBL were cultured at a cell density of 1 × 10^6 cells mL^-1 for 30-36 h at an incubation temperature of 30 ℃. The IL2 like activity was purified by DEAE-Sepharose anion exchange chromatography and recorded between 70-130 mM NaCI with peak activity at 110 Mm NaCI. The molecular weight of the factor responsible for IL2 like activity was found to be 15-17 KD.
文摘Gnaphalium oxyphyllum DC is a medicinal plant whose common uses by Mexican people include the treatment of cancer. The toxicity of the aqueous and organic fractions as well as the aqueous decoction of G. oxyphyllum var. natalie F. J. Espinosa were assessed on three human cancer cell lines as well as in blood cells in healthy human lymphocyte cultures. Cytotoxic activity was assessed by the Sulforhodamine B method on HeLa （human cervical carcinoma）, T47D （human breast carcinoma） and 22Rvl （human prostate carcinoma cancer）. Colchicine was used as positive control. The decoction was also tested on lymphocytes from healthy donors through the mitotic index as biomarker. We used whole blood for these cultures and estimated the effect of the extract on platelets, leukocytes and erythrocytes. The aqueous decoction was cytotoxic （EDs0 〈 20 μg/mL） on the three cancer cell lines. The mitotic index in the exposed lymphocyte cultures did not significantly differ from the control nor the blood counts showed any difference between the experimental and control cultures. These results prove that the toxic effect of the aqueous decoction of G. oxyphyllum var. natalie is specific for cancer cell lines.
