目的:研究血中性粒细胞/淋巴细胞比值(neutrophil-lymphocyte ratio,NLR)及其变化率与重症医学科(intensive care unit,ICU)脓毒症患者预后之间的关系。方法 :选择入住ICU的脓毒症患者94例,根据预后分为死亡组(25例)和存活组(69例),评...目的:研究血中性粒细胞/淋巴细胞比值(neutrophil-lymphocyte ratio,NLR)及其变化率与重症医学科(intensive care unit,ICU)脓毒症患者预后之间的关系。方法 :选择入住ICU的脓毒症患者94例,根据预后分为死亡组(25例)和存活组(69例),评估两组NLR水平及其变化率与预后的关系。结果:ICU脓毒症患者死亡组和存活组入科时NLR分别为16.69±13.99和16.27±15.72,差异无统计学意义(P>0.05);48 h NLR分别为24.31±15.53和12.53±10.64,差异有统计学意义(P<0.05);NLR变化率分别为0.92±1.57和-0.11±0.62,差异有统计学意义(P<0.01);急性生理学与慢性健康状况评分系统Ⅱ(score of acute pathologic and chronic health evaluationⅡ,APACHEⅡ)评分分别为27.00±5.81和19.99±4.41,差异有统计学意义(P<0.01)。结论:ICU脓毒症患者48 h NLR、NLR变化率与预后相关。展开更多
目的探讨同种异体原位肝移植患者围手术期免疫细胞功能(ImmuKnow值)、T淋巴细胞形态学变化规律及两者之间的关系。方法选择2009年9月至2010年1月28例行同种异体原位肝移植的患者(肝移植组)和20例健康成人(对照组)进行研究。肝移植组于...目的探讨同种异体原位肝移植患者围手术期免疫细胞功能(ImmuKnow值)、T淋巴细胞形态学变化规律及两者之间的关系。方法选择2009年9月至2010年1月28例行同种异体原位肝移植的患者(肝移植组)和20例健康成人(对照组)进行研究。肝移植组于术前及术后第1、3、5、10、15天抽取外周静脉血,对照组入组时采血,进行ImmuKnow检测和T淋巴细胞形态变化指数检测(morphologic change index of T-lymphocyte,TMCI)。结果 Pearson相关分析显示,肝移植组及对照组共188个ImmuKnow值与对应的TMCI之间存在线性相关,(r=0.166,P=0.023)。结论 Immuknow值与TMCI相关,TMCI可作为临床评估患者T淋巴细胞功能的参考指标,结合其他相关检查,可有效指导肝移植受体免疫抑制剂的个体化应用。展开更多
The transgenic carp were produced by micro-injection of CAgcGHc into the fertilized eggs. Observation of the thymus development between the transgenics and non- transgenic controls was carried out. The thymus of one-y...The transgenic carp were produced by micro-injection of CAgcGHc into the fertilized eggs. Observation of the thymus development between the transgenics and non- transgenic controls was carried out. The thymus of one-year- old transgenics F1 showed a great increase in both size and weight. The unilateral thymus of the transgenics weighed from 190 to 295 mg with average 218.6 mg, whereas the uni-lateral thymus of the controls weighed 20—81 mg with av-erage 42.5 mg; i.e. the thymus weight in the transgenics was 5.14 fold over that in the controls. The index of thymus/body weight in the transgenics was 2.97 fold over the controls. Light microscopy observation indicated that the thymus of the transgenics well developed with the thickened outer re-gion and compactly arranged thymocytes, while the thymus in the controls were degenerating with the thinned outer region, scattered thymocytes and groups of fatty cells. Fur-ther analysis with the electron microscopy revealed that pro-liferous cells in the transgenics were mainly small lympho-cytes and no pathological changes were found. The results confirmed that the 揂ll-fish?GH-transgene promotes thy-mus development and thymocyte proliferation, and retards thymus degeneration. The study has laid a foundation for further analysis of the immunobiological function in GH- transgenic carp.展开更多
文摘目的:研究血中性粒细胞/淋巴细胞比值(neutrophil-lymphocyte ratio,NLR)及其变化率与重症医学科(intensive care unit,ICU)脓毒症患者预后之间的关系。方法 :选择入住ICU的脓毒症患者94例,根据预后分为死亡组(25例)和存活组(69例),评估两组NLR水平及其变化率与预后的关系。结果:ICU脓毒症患者死亡组和存活组入科时NLR分别为16.69±13.99和16.27±15.72,差异无统计学意义(P>0.05);48 h NLR分别为24.31±15.53和12.53±10.64,差异有统计学意义(P<0.05);NLR变化率分别为0.92±1.57和-0.11±0.62,差异有统计学意义(P<0.01);急性生理学与慢性健康状况评分系统Ⅱ(score of acute pathologic and chronic health evaluationⅡ,APACHEⅡ)评分分别为27.00±5.81和19.99±4.41,差异有统计学意义(P<0.01)。结论:ICU脓毒症患者48 h NLR、NLR变化率与预后相关。
文摘目的探讨同种异体原位肝移植患者围手术期免疫细胞功能(ImmuKnow值)、T淋巴细胞形态学变化规律及两者之间的关系。方法选择2009年9月至2010年1月28例行同种异体原位肝移植的患者(肝移植组)和20例健康成人(对照组)进行研究。肝移植组于术前及术后第1、3、5、10、15天抽取外周静脉血,对照组入组时采血,进行ImmuKnow检测和T淋巴细胞形态变化指数检测(morphologic change index of T-lymphocyte,TMCI)。结果 Pearson相关分析显示,肝移植组及对照组共188个ImmuKnow值与对应的TMCI之间存在线性相关,(r=0.166,P=0.023)。结论 Immuknow值与TMCI相关,TMCI可作为临床评估患者T淋巴细胞功能的参考指标,结合其他相关检查,可有效指导肝移植受体免疫抑制剂的个体化应用。
基金supported by the State High-Techmology Program and the Special Funds of the Major State Basic Research of China(Grant Nos.2001AA212281 and 2001CB109006)the National Natural Science Foundation of China(Grant Nos.301 30050 and 30070588).
文摘The transgenic carp were produced by micro-injection of CAgcGHc into the fertilized eggs. Observation of the thymus development between the transgenics and non- transgenic controls was carried out. The thymus of one-year- old transgenics F1 showed a great increase in both size and weight. The unilateral thymus of the transgenics weighed from 190 to 295 mg with average 218.6 mg, whereas the uni-lateral thymus of the controls weighed 20—81 mg with av-erage 42.5 mg; i.e. the thymus weight in the transgenics was 5.14 fold over that in the controls. The index of thymus/body weight in the transgenics was 2.97 fold over the controls. Light microscopy observation indicated that the thymus of the transgenics well developed with the thickened outer re-gion and compactly arranged thymocytes, while the thymus in the controls were degenerating with the thinned outer region, scattered thymocytes and groups of fatty cells. Fur-ther analysis with the electron microscopy revealed that pro-liferous cells in the transgenics were mainly small lympho-cytes and no pathological changes were found. The results confirmed that the 揂ll-fish?GH-transgene promotes thy-mus development and thymocyte proliferation, and retards thymus degeneration. The study has laid a foundation for further analysis of the immunobiological function in GH- transgenic carp.
