目的探讨血管入路联合淋巴结示踪剂在降结肠癌根治术中淋巴结分检的技术优势。方法 2015年6月至2016年12月,河南省肿瘤医院普外科60例降结肠癌患者随机分为淋巴结示踪组(观察组)和非示踪组(对照组),对照两组检出淋巴结总数、平均检出淋...目的探讨血管入路联合淋巴结示踪剂在降结肠癌根治术中淋巴结分检的技术优势。方法 2015年6月至2016年12月,河南省肿瘤医院普外科60例降结肠癌患者随机分为淋巴结示踪组(观察组)和非示踪组(对照组),对照两组检出淋巴结总数、平均检出淋巴结数、淋巴结小于12枚人数、阳性淋巴结数、淋巴结阳性率、患者转移率、平均直径小于5 mm数、第一、二、三站淋巴结数及阳性淋巴结数目和淋巴结分检时间等指标。结果观察组检出淋巴结总数和阳性淋巴结总数均高于对照组(554:427 vs 95:59);平均检出淋巴结数(21.50±6.03 vs 13.61±1.97,t=25.970,P<0.001)、淋巴结分检时间(min)(12.31±2.5 vs 17.4±3.2,t=13.491,P<0.001)、平均直径小于5 mm数(4.98±1.76 vs2.86±1.21,t=8.279,P<0.001)、淋巴结小于12枚人数[0%(0/28)vs 16.7%(5/32),Χ~2=4.773,P=0.029]、第一站淋巴结检出数目(11.32±4.41 vs 5.71±1.91,t=15.128,P<0.001)、第二站淋巴结数(7.49±1.78 vs 5.58±1.68,t=10.358,P=0.001)、第一站阳性淋巴结检出数目(5.11±3.48 vs2.19±0.83,t=4.300,P<0.001)、第二站阳性淋巴结数(3.69±2.32 vs 1.91±0.61,t=3.501,P=0.001)等指标差异有显著性统计学意义;第三站淋巴结数(4.21±1.43 vs 4.06±1.64,t=0.714,P=0.476)及第三站阳性淋巴结数(2.50±1.60 vs 1.60±0.52,t=1.680,P=0.112)等差异无显著性统计学意义。结论降结肠癌根治术中采用血管入路联合纳米碳淋巴结示踪方法可提高淋巴结分检效率并获取更多淋巴结数目多,使术后病理分期更加准确。展开更多
Objective： To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer （EOC） cells （SKOV3-PM4） with directional highly lymphatic metastasis. Methods： Supernatants of four groups of...Objective： To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer （EOC） cells （SKOV3-PM4） with directional highly lymphatic metastasis. Methods： Supernatants of four groups of cultured cells, namely, SKOV3 （A）, SKOV3＋HLEC （B）, SKOV3-PM4 （C）, SKOV3-PM4＋HLEC （D）, were collected, and their proteins were detected by antibody arrays and iTRAOcZD-LC-MALDI- TOF/TOF/MS. Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA. Results： Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C （compared with group A）, whereas IGFBP7 and SPARC were downregulated in group D （compared with group C）. Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other. Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of patients with ovarian cancers, benign tumors, and control groups. Two proteins were upegulated in the first group. VEGFA in the control group was downregulated. For IGFBP, upregulation in the control group and down-regulation in the first group were also observed. Conclusion： The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors. The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.展开更多
AIM:To investigate the ezrin expression in normal colorectal mucosa and colorectal cancer tissues, and study the correlation between ezrin expression in colorectal cancer tissues and tumor invasion and metastasis.METH...AIM:To investigate the ezrin expression in normal colorectal mucosa and colorectal cancer tissues, and study the correlation between ezrin expression in colorectal cancer tissues and tumor invasion and metastasis.METHODS: Eighty paraffin-embedded cancer tissue samples were selected from primary colorectal adenocarcinoma. Twenty-eight patients had well-differentiated, 22 had moderately differentiated and 30 had poorly differentiated adenocarcinoma. Forty-five patients and 35 patients had lymph node metastasis. Forty-five patients were of Dukes A to B stage, and 35 were of C to D stage. Another 22 paraffi n-embedded tissue blocks of normal colorectal epithelium (>5 cm away from the edge of the tumor) were selected as the control group. All patients with colorectal cancer were treated surgically and diagnosed histologically, without preoperative chemotherapy or radiotherapy. The immunohistochemistry was used to detect the ezrin expression in paraffin-embedded normal colorectal mucosa tissues and colorectal cancer tissue samples.RESULTS: Ezrin expression in colorectal cancer was significantly higher than in normal colorectal mucosa (75.00% vs 9.09%, P<0.01), and there was a close relationship between ezrin expression and the degree of tumor differentiation, lymph node metastasis and Dukes stage (88.46% vs 50.00%, P<0.01; 94.28% vs 51.11%, P<0.01; 94.28% vs 51.11%, P<0.01).CONCLUSION: Ezrin expression is obviously higher in colorectal cancer tissues than in normal colorectal mucosa tissues, and the high level of ezrin expression is closely related to the colorectal cancer invasion and metastasis process.展开更多
OBJECTIVE To evaluate the impact of the number of negative lymph nodes on disease free survival （DFS） in patients with locally advanced gastric cancer. METHODS A total of 485 patients who underwent surgery for local...OBJECTIVE To evaluate the impact of the number of negative lymph nodes on disease free survival （DFS） in patients with locally advanced gastric cancer. METHODS A total of 485 patients who underwent surgery for locally advanced gastric cancer （pT3N0-2M0） and had a DFS at least 6 months were enrolled in this retrospective study. The medical records of the patients were reviewed in detail, and the characteristics of the patients and the findings of pathologic examination were analyzed in order to find the potential association with DFS. Subgroup analysis according to pathologic stage was performed. Multivariate analysis using the COX regression method was also conducted in order to identify the independent prognostic factors. The Kaplan-Meier method was used to plot DFS curves. The DFS rate was compared in each subgroup. RESULTS COX regression analysis showed that the DFS rate of gastric cancer patients with pathologic stage T3N0-2M0 was significantly associated with age, degree of tumor differentiation, tumor location as well as the number of negative lymph nodes. Among patients with stage T3NOM0 disease, who had 1-4 and 5 or more negative lymph nodes, the 2-year DFS rate was 8.3% and 55.6%, respectively. Meanwhile, the 3-year DFS rates of the same group of patients was 0% and 24.9%, respectively （P = 0.025）. In the T3NIM0 subgroup, the 2-year DFS rate of patients with 3 or fewer, 4-9, and 10 or more negative lymph nodes was 17.3%, 39.1%, 52.6%, respectively. The 3-year DFS rate in this group was 4.2%, 6.0%, 17.1%, respectively （P 〈 0.001）. In the T3N2M0 subgroup, the 2-year DFS rate of patients with 7 or fewer and 8 or more negative lymph nodes was 11.5% and 35%, respectively. The 3-year DFS rate of the same group of patients with 8 or more negative lymph nodes was also 5%, respectively; P = 0.015）. significantly improved （0.8% vs. CONCLUSION For gastric cancer patients with pathologic stage T3N0-2M0, the number of negative lymph nodes is an independent prognostic factor for DFS. The number of negative lymph nodes may reflect the level of regional lymph node dissection or the accuracy of the pathologic staging.展开更多
Dear Editor,Kikuchi-Fujimoto disease(KFD),or histiocytic necrotizing lymphadenitis,was initially described in 1972 by Drs.Kikuchi and Fujimoto(Shirakusa et al.,1988).The main clinical manifestations are fever and cerv...Dear Editor,Kikuchi-Fujimoto disease(KFD),or histiocytic necrotizing lymphadenitis,was initially described in 1972 by Drs.Kikuchi and Fujimoto(Shirakusa et al.,1988).The main clinical manifestations are fever and cervical lymphadenopathy with or without pain,sometimes accompanied by tachycardia,nausea and symptomatic infiltrative erythematous lesions.The diagnosis of KFD is usually based on characteristic lymph node histologic findings of coagulative necrosis,histiocytic infiltrate,loss of nodal architecture,展开更多
文摘目的探讨血管入路联合淋巴结示踪剂在降结肠癌根治术中淋巴结分检的技术优势。方法 2015年6月至2016年12月,河南省肿瘤医院普外科60例降结肠癌患者随机分为淋巴结示踪组(观察组)和非示踪组(对照组),对照两组检出淋巴结总数、平均检出淋巴结数、淋巴结小于12枚人数、阳性淋巴结数、淋巴结阳性率、患者转移率、平均直径小于5 mm数、第一、二、三站淋巴结数及阳性淋巴结数目和淋巴结分检时间等指标。结果观察组检出淋巴结总数和阳性淋巴结总数均高于对照组(554:427 vs 95:59);平均检出淋巴结数(21.50±6.03 vs 13.61±1.97,t=25.970,P<0.001)、淋巴结分检时间(min)(12.31±2.5 vs 17.4±3.2,t=13.491,P<0.001)、平均直径小于5 mm数(4.98±1.76 vs2.86±1.21,t=8.279,P<0.001)、淋巴结小于12枚人数[0%(0/28)vs 16.7%(5/32),Χ~2=4.773,P=0.029]、第一站淋巴结检出数目(11.32±4.41 vs 5.71±1.91,t=15.128,P<0.001)、第二站淋巴结数(7.49±1.78 vs 5.58±1.68,t=10.358,P=0.001)、第一站阳性淋巴结检出数目(5.11±3.48 vs2.19±0.83,t=4.300,P<0.001)、第二站阳性淋巴结数(3.69±2.32 vs 1.91±0.61,t=3.501,P=0.001)等指标差异有显著性统计学意义;第三站淋巴结数(4.21±1.43 vs 4.06±1.64,t=0.714,P=0.476)及第三站阳性淋巴结数(2.50±1.60 vs 1.60±0.52,t=1.680,P=0.112)等差异无显著性统计学意义。结论降结肠癌根治术中采用血管入路联合纳米碳淋巴结示踪方法可提高淋巴结分检效率并获取更多淋巴结数目多,使术后病理分期更加准确。
基金supported by the National Natural Science Foundation of China (Grant No. 81060218)Guangxi Natural Science Foundation (Grant No. 2012GXNSFAA053157)
文摘Objective： To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer （EOC） cells （SKOV3-PM4） with directional highly lymphatic metastasis. Methods： Supernatants of four groups of cultured cells, namely, SKOV3 （A）, SKOV3＋HLEC （B）, SKOV3-PM4 （C）, SKOV3-PM4＋HLEC （D）, were collected, and their proteins were detected by antibody arrays and iTRAOcZD-LC-MALDI- TOF/TOF/MS. Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA. Results： Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C （compared with group A）, whereas IGFBP7 and SPARC were downregulated in group D （compared with group C）. Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other. Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of patients with ovarian cancers, benign tumors, and control groups. Two proteins were upegulated in the first group. VEGFA in the control group was downregulated. For IGFBP, upregulation in the control group and down-regulation in the first group were also observed. Conclusion： The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors. The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.
基金Supported by Natural Science Foundation of Shanghai,No.04ZB14072
文摘AIM:To investigate the ezrin expression in normal colorectal mucosa and colorectal cancer tissues, and study the correlation between ezrin expression in colorectal cancer tissues and tumor invasion and metastasis.METHODS: Eighty paraffin-embedded cancer tissue samples were selected from primary colorectal adenocarcinoma. Twenty-eight patients had well-differentiated, 22 had moderately differentiated and 30 had poorly differentiated adenocarcinoma. Forty-five patients and 35 patients had lymph node metastasis. Forty-five patients were of Dukes A to B stage, and 35 were of C to D stage. Another 22 paraffi n-embedded tissue blocks of normal colorectal epithelium (>5 cm away from the edge of the tumor) were selected as the control group. All patients with colorectal cancer were treated surgically and diagnosed histologically, without preoperative chemotherapy or radiotherapy. The immunohistochemistry was used to detect the ezrin expression in paraffin-embedded normal colorectal mucosa tissues and colorectal cancer tissue samples.RESULTS: Ezrin expression in colorectal cancer was significantly higher than in normal colorectal mucosa (75.00% vs 9.09%, P<0.01), and there was a close relationship between ezrin expression and the degree of tumor differentiation, lymph node metastasis and Dukes stage (88.46% vs 50.00%, P<0.01; 94.28% vs 51.11%, P<0.01; 94.28% vs 51.11%, P<0.01).CONCLUSION: Ezrin expression is obviously higher in colorectal cancer tissues than in normal colorectal mucosa tissues, and the high level of ezrin expression is closely related to the colorectal cancer invasion and metastasis process.
文摘OBJECTIVE To evaluate the impact of the number of negative lymph nodes on disease free survival （DFS） in patients with locally advanced gastric cancer. METHODS A total of 485 patients who underwent surgery for locally advanced gastric cancer （pT3N0-2M0） and had a DFS at least 6 months were enrolled in this retrospective study. The medical records of the patients were reviewed in detail, and the characteristics of the patients and the findings of pathologic examination were analyzed in order to find the potential association with DFS. Subgroup analysis according to pathologic stage was performed. Multivariate analysis using the COX regression method was also conducted in order to identify the independent prognostic factors. The Kaplan-Meier method was used to plot DFS curves. The DFS rate was compared in each subgroup. RESULTS COX regression analysis showed that the DFS rate of gastric cancer patients with pathologic stage T3N0-2M0 was significantly associated with age, degree of tumor differentiation, tumor location as well as the number of negative lymph nodes. Among patients with stage T3NOM0 disease, who had 1-4 and 5 or more negative lymph nodes, the 2-year DFS rate was 8.3% and 55.6%, respectively. Meanwhile, the 3-year DFS rates of the same group of patients was 0% and 24.9%, respectively （P = 0.025）. In the T3NIM0 subgroup, the 2-year DFS rate of patients with 3 or fewer, 4-9, and 10 or more negative lymph nodes was 17.3%, 39.1%, 52.6%, respectively. The 3-year DFS rate in this group was 4.2%, 6.0%, 17.1%, respectively （P 〈 0.001）. In the T3N2M0 subgroup, the 2-year DFS rate of patients with 7 or fewer and 8 or more negative lymph nodes was 11.5% and 35%, respectively. The 3-year DFS rate of the same group of patients with 8 or more negative lymph nodes was also 5%, respectively; P = 0.015）. significantly improved （0.8% vs. CONCLUSION For gastric cancer patients with pathologic stage T3N0-2M0, the number of negative lymph nodes is an independent prognostic factor for DFS. The number of negative lymph nodes may reflect the level of regional lymph node dissection or the accuracy of the pathologic staging.
基金supported in part by Beijing Natural Science Foundation (7162060 to ZX)Basic and Clinic Science Foundation of Capital Medical University (16JL22 to ZX)+2 种基金Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding (ZYLX201601 to LM)Health and Family Planning Research and Development Foundation of Shunyi District (Wsjzkyfzzx-z-02 to ZX)the US National Institutes of Health grant (P30-CA008748 to YWT)
文摘Dear Editor,Kikuchi-Fujimoto disease(KFD),or histiocytic necrotizing lymphadenitis,was initially described in 1972 by Drs.Kikuchi and Fujimoto(Shirakusa et al.,1988).The main clinical manifestations are fever and cervical lymphadenopathy with or without pain,sometimes accompanied by tachycardia,nausea and symptomatic infiltrative erythematous lesions.The diagnosis of KFD is usually based on characteristic lymph node histologic findings of coagulative necrosis,histiocytic infiltrate,loss of nodal architecture,
