Eight genotypes(A-H) of hepatitis B virus(HBV) have been identified.However,the impact of different genotypes on the clinical course of hepatitis B infection remains controversial.We investigated the frequency and cli...Eight genotypes(A-H) of hepatitis B virus(HBV) have been identified.However,the impact of different genotypes on the clinical course of hepatitis B infection remains controversial.We investigated the frequency and clinical outcome of HBV genotypes and genotype mixtures in HBV-infected patients from Vietnam,Europe,and Africa.In addition,we analyzed the effects of genotype mixtures on alterations in in vitro viral replication.In Asian patients,seven genotypes(A-G) were detected,with A,C,and D predominating.In European and African patients,only genotypes A,C,D,and G were identified.Genotype mixtures were more frequently encountered in African than in Asian(P =.01) and European patients(P =.06) .In Asian patients,the predominant genotype mixtures included A/C and C/D,compared to C/D in European and A/D in African patients.Genotype A was more frequent in asymptomatic compared with symptomatic patients(P <.0001) .Genotype C was more frequent in patients with hepatocellular carcinoma(HCC;P =.02) .Genotype mixtures were more frequently encountered in patients with chronic hepatitis in comparison to patients with acute hepatitis B(P =.015) ,liver cirrhosis(P =.013) ,and HCC(P =.002) .Viral loads in patients infected with genotype mixtures were significantly higher in comparison to patients with a single genotype(P =.019) .Genotype mixtures were also associated with increased in vitro HBV replication.In conclusion,infection with mixtures of HBV genotypes is frequent in Asia,Africa,and Europe.Differences in the replication-phenotype of single genotypes compared to genotype-mixtures suggest that co-infection with different HBV-genotypes is associated with altered pathogenesis and clinical outcome.展开更多
[Objective] The aim was to carry out the genetic analysis on plant height of rice（Oryza sativa L.）cultivated in different seasons.[Method] Three rice parents with great difference in plant height including CB1（83.1...[Objective] The aim was to carry out the genetic analysis on plant height of rice（Oryza sativa L.）cultivated in different seasons.[Method] Three rice parents with great difference in plant height including CB1（83.1 cm）,CB4（105.5 cm）and CB7（115.6 cm）were chosen to construct two parental combinations：CB1×CB4 and CB7×CB4,and the corresponding filial generations P1,F1,P2,B1,B2 and F2 were obtained.The 6 populations were planted in middle and late seasons respectively to measure their height traits.The Akaike＇s information criterion（AIC）of the mixed major gene and polygene model was used to indentify the existence of major genes affecting quantitative traits in B1,B2,F2 populations.When the major genes existed,the genetic effects of the major genes and polygenes and their genetic variance were estimated through segregation analysis.[Result] One additive major gene plus additive-dominance polygenes was the most fitted genetic model for the trait in all B1,B2,F2 populations in two planting seasons.The heritability values of the major genes varied from 38.63% to 78.53% and those of polygenes varied from 1.72% to 36.04%,and the total heritability values were 45.52-92.93%.The additive effect d value of the two genetic populations under two planting seasons was-4.56,-9.16,-7.19,and-9.38,respectively,as suggested that additive effect of the major genes would decrease the express of the plant height trait.[Conclusion] The heritability of plant height trait was affected by planting seasons and the combinations clearly as a whole.展开更多
A DH population derived from C49S-87/01Y1-1069 was used to study the inheritance of wheat haploid embryo production frequency（EPF） in wheat × maize cross with the mixed major gene and polygene inheritance model...A DH population derived from C49S-87/01Y1-1069 was used to study the inheritance of wheat haploid embryo production frequency（EPF） in wheat × maize cross with the mixed major gene and polygene inheritance model of quantitative traits. The results showed that the EPF of wheat × maize cross was controlled by two dominant epistatic genes and polygene with gene effects of 1.95 for the first major gene, 6.69 for the second one and 2.80 for the polygene. The inheritability of major genes was as high as 72.09%, suggesting that the differences in EPF among wheat materials were mainly influenced by genotype. However, non-genetic factors were still important, especially for wheat materials with low EPF.展开更多
Microarray has become increasingly popular biotechnology in biological and medical researches, and has been widely applied in classification of treatment subtypes using expression patterns of biomarkers. We developed ...Microarray has become increasingly popular biotechnology in biological and medical researches, and has been widely applied in classification of treatment subtypes using expression patterns of biomarkers. We developed a statistical procedure to identify expression biomarkers for treatment subtype classification by constructing an F-statistic based on Henderson method Ⅲ. Monte Carlo simulations were conducted to examine the robustness and efficiency of the proposed method. Simulation results showed that our method could provide satisfying power of identifying differentially expressed genes （DEGs） with false discovery rate （FDR） lower than the given type I error rate. In addition, we analyzed a leukemia dataset collected from 38 leukemia patients with 27 samples diagnosed as acute lymphoblastic leukemia （ALL） and 11 samples as acute myeloid leukemia （AML）. We compared our results with those from the methods of significance analysis of microarray （SAM） and microarray analysis of variance （MAANOVA）. Among these three methods, only expression biomarkers identified by our method can precisely identify the three human acute leukemia subtypes.展开更多
文摘Eight genotypes(A-H) of hepatitis B virus(HBV) have been identified.However,the impact of different genotypes on the clinical course of hepatitis B infection remains controversial.We investigated the frequency and clinical outcome of HBV genotypes and genotype mixtures in HBV-infected patients from Vietnam,Europe,and Africa.In addition,we analyzed the effects of genotype mixtures on alterations in in vitro viral replication.In Asian patients,seven genotypes(A-G) were detected,with A,C,and D predominating.In European and African patients,only genotypes A,C,D,and G were identified.Genotype mixtures were more frequently encountered in African than in Asian(P =.01) and European patients(P =.06) .In Asian patients,the predominant genotype mixtures included A/C and C/D,compared to C/D in European and A/D in African patients.Genotype A was more frequent in asymptomatic compared with symptomatic patients(P <.0001) .Genotype C was more frequent in patients with hepatocellular carcinoma(HCC;P =.02) .Genotype mixtures were more frequently encountered in patients with chronic hepatitis in comparison to patients with acute hepatitis B(P =.015) ,liver cirrhosis(P =.013) ,and HCC(P =.002) .Viral loads in patients infected with genotype mixtures were significantly higher in comparison to patients with a single genotype(P =.019) .Genotype mixtures were also associated with increased in vitro HBV replication.In conclusion,infection with mixtures of HBV genotypes is frequent in Asia,Africa,and Europe.Differences in the replication-phenotype of single genotypes compared to genotype-mixtures suggest that co-infection with different HBV-genotypes is associated with altered pathogenesis and clinical outcome.
基金Supported by the Science and Technology Project of Food Production in Jiangxi Province(2006BAD02A04)~~
文摘[Objective] The aim was to carry out the genetic analysis on plant height of rice（Oryza sativa L.）cultivated in different seasons.[Method] Three rice parents with great difference in plant height including CB1（83.1 cm）,CB4（105.5 cm）and CB7（115.6 cm）were chosen to construct two parental combinations：CB1×CB4 and CB7×CB4,and the corresponding filial generations P1,F1,P2,B1,B2 and F2 were obtained.The 6 populations were planted in middle and late seasons respectively to measure their height traits.The Akaike＇s information criterion（AIC）of the mixed major gene and polygene model was used to indentify the existence of major genes affecting quantitative traits in B1,B2,F2 populations.When the major genes existed,the genetic effects of the major genes and polygenes and their genetic variance were estimated through segregation analysis.[Result] One additive major gene plus additive-dominance polygenes was the most fitted genetic model for the trait in all B1,B2,F2 populations in two planting seasons.The heritability values of the major genes varied from 38.63% to 78.53% and those of polygenes varied from 1.72% to 36.04%,and the total heritability values were 45.52-92.93%.The additive effect d value of the two genetic populations under two planting seasons was-4.56,-9.16,-7.19,and-9.38,respectively,as suggested that additive effect of the major genes would decrease the express of the plant height trait.[Conclusion] The heritability of plant height trait was affected by planting seasons and the combinations clearly as a whole.
基金Supported by National High Technology Research and Development Program of China(863 Program)(2011AA10A106)Yunnan Provincial Fund for Applied Basic Researches(2010CC001)Key New Product Development Plan of Yunnan Province(2012BB015)~~
文摘A DH population derived from C49S-87/01Y1-1069 was used to study the inheritance of wheat haploid embryo production frequency（EPF） in wheat × maize cross with the mixed major gene and polygene inheritance model of quantitative traits. The results showed that the EPF of wheat × maize cross was controlled by two dominant epistatic genes and polygene with gene effects of 1.95 for the first major gene, 6.69 for the second one and 2.80 for the polygene. The inheritability of major genes was as high as 72.09%, suggesting that the differences in EPF among wheat materials were mainly influenced by genotype. However, non-genetic factors were still important, especially for wheat materials with low EPF.
基金Project partly supported by the National Basic Research Program(973) of China (No. 2004CB117306) and the National Natural Sci-ence Foundation of China (No. 2002AA234031)
文摘Microarray has become increasingly popular biotechnology in biological and medical researches, and has been widely applied in classification of treatment subtypes using expression patterns of biomarkers. We developed a statistical procedure to identify expression biomarkers for treatment subtype classification by constructing an F-statistic based on Henderson method Ⅲ. Monte Carlo simulations were conducted to examine the robustness and efficiency of the proposed method. Simulation results showed that our method could provide satisfying power of identifying differentially expressed genes （DEGs） with false discovery rate （FDR） lower than the given type I error rate. In addition, we analyzed a leukemia dataset collected from 38 leukemia patients with 27 samples diagnosed as acute lymphoblastic leukemia （ALL） and 11 samples as acute myeloid leukemia （AML）. We compared our results with those from the methods of significance analysis of microarray （SAM） and microarray analysis of variance （MAANOVA）. Among these three methods, only expression biomarkers identified by our method can precisely identify the three human acute leukemia subtypes.
