To screen the high efficient mixed culture and understand the bioleaching behaviors of mixed culture for low-grade copper sulfide ore bioleaching,ten mixed cultures were collected and screened from different acid mine...To screen the high efficient mixed culture and understand the bioleaching behaviors of mixed culture for low-grade copper sulfide ore bioleaching,ten mixed cultures were collected and screened from different acid mine drainages obtained from sulfide mines of China.The leaching rate was set as criterion to screen the mixed culture and the metagenomic approach.Community genome array（CGA） was used for analyzing the mixed culture microbial community shift during the bioleaching process.The results indicate that the mixed culture obtained from Yinshan（YS） lead-zinc mine in Dexing of Jiangxi province in China reaches the maximum copper extraction（68.89%） during the one bioleaching period of 24 d.CGA results show that YS culture contains nine kinds of bacteria which are belong to six divisions,and the microbial community structure is changing during the bioleaching process.This provides a good way to accelerate the bioleaching process and reveals the microbial community shift during the bioleaching process.展开更多
The catalysis of four carbon materials including artificial graphite(AG), carbon black(CB), activated carbon(AC) and carbon nanotube(CN) on chalcopyrite bioleaching by mixed moderate thermophiles was comparatively inv...The catalysis of four carbon materials including artificial graphite(AG), carbon black(CB), activated carbon(AC) and carbon nanotube(CN) on chalcopyrite bioleaching by mixed moderate thermophiles was comparatively investigated. In AC and AG added bioleaching groups, low solution pH and suitable redox potential values, high total iron and ferric iron concentrations, and large number of adsorbed bacteria were obtained, resulting in high copper extractions. CB and CN inhibited the growth of bioleaching bacteria and led to the low bioleaching efficiency.X-ray diffraction analysis showed that jarosite and sulfur film were the main components of passivation layer with the addition of AG and AC,but did not hinder the dissolution of chalcopyrite. Microbial community structures of free and attached cells in AC and AG added groups changed dramatically compared with mixed moderate thermophiles. The sulfur-oxidizing bacteria of A. caldus S1 strain dominated the microbial community(93%-98%) at the end of bioleaching.The iron-oxidizing bacteria of L.ferriphilum YSK only accounted for low percentage(1%-2%).展开更多
基金Project(50774102)supported by the National Natural Science Foundation of ChinaProject(2004CB619204)supported by the National Basic Research Program of China
文摘To screen the high efficient mixed culture and understand the bioleaching behaviors of mixed culture for low-grade copper sulfide ore bioleaching,ten mixed cultures were collected and screened from different acid mine drainages obtained from sulfide mines of China.The leaching rate was set as criterion to screen the mixed culture and the metagenomic approach.Community genome array（CGA） was used for analyzing the mixed culture microbial community shift during the bioleaching process.The results indicate that the mixed culture obtained from Yinshan（YS） lead-zinc mine in Dexing of Jiangxi province in China reaches the maximum copper extraction（68.89%） during the one bioleaching period of 24 d.CGA results show that YS culture contains nine kinds of bacteria which are belong to six divisions,and the microbial community structure is changing during the bioleaching process.This provides a good way to accelerate the bioleaching process and reveals the microbial community shift during the bioleaching process.
基金Project(31570113)supported by the National Natural Science Foundation of ChinaProject(2016YFB0101310)supported by the National Key Research and Development Program of China
文摘The catalysis of four carbon materials including artificial graphite(AG), carbon black(CB), activated carbon(AC) and carbon nanotube(CN) on chalcopyrite bioleaching by mixed moderate thermophiles was comparatively investigated. In AC and AG added bioleaching groups, low solution pH and suitable redox potential values, high total iron and ferric iron concentrations, and large number of adsorbed bacteria were obtained, resulting in high copper extractions. CB and CN inhibited the growth of bioleaching bacteria and led to the low bioleaching efficiency.X-ray diffraction analysis showed that jarosite and sulfur film were the main components of passivation layer with the addition of AG and AC,but did not hinder the dissolution of chalcopyrite. Microbial community structures of free and attached cells in AC and AG added groups changed dramatically compared with mixed moderate thermophiles. The sulfur-oxidizing bacteria of A. caldus S1 strain dominated the microbial community(93%-98%) at the end of bioleaching.The iron-oxidizing bacteria of L.ferriphilum YSK only accounted for low percentage(1%-2%).
