AIM： To create a rabbit model of pediatric nonalcoholic steatohepatitis （NASH） and to evaluate the role of adiponectin in the process. METHODS： Thirty-two specific pathogen-free male New Zealand rabbits were divid...AIM： To create a rabbit model of pediatric nonalcoholic steatohepatitis （NASH） and to evaluate the role of adiponectin in the process. METHODS： Thirty-two specific pathogen-free male New Zealand rabbits were divided randomly into three groups： （1） the normal control group （n = 10） was fed with standard diet for 12 wk; （2） the model group A （n = 11）; and （3） model group B （n = 11） were fed with a highfat diet （standard diet ＋ 10% lard ＋ 2% cholesterol） for 8 and 12 wk, respectively. Hepatic histological changes were observed and biochemical parameters as well as serum levels of adiponectin, interleukin （IL）-6, IL-10 and tumor necrosis factor （TNF）-α were measured. RESULTS： Typical histological hepatic lesions of NASH were observed in both model groups described as liver steatosis, liver inflammatory infiltration, cytologic ballooning, perisinusoidal fibrosis and overall fibrosis. Compared with the normal control group, there were significant increases in model groups A and B in weight gain （1097.2 ± 72.3, 1360.5± 107.6 vs 928.0 ±58.1, P 〈 0.05, P 〈 0.01）, liver weight （93.81±6.64, 104.6±4.42 vs 54.4±1.71, P 〈 0.01）, Lg （ALT） （1.9±0.29, 1.84± 0.28 vs 1.60±0.17, P 〈 0.01）, and Lg （TG） （1.03 ±0.24, 1.16 ±0.33 vs 0.00 ±0.16, P 〈 0.01）. Weight gain was much more in model group B than in model group A （1360.5± 107.6 vs 1097.2 ±72.3, P 〈 0.05）. But, there was no significant difference between the two groups concerning the other indexes. Pro-inflammatory cytokines （IL-6 and TNF-α） increased in model group B compared with that of control and model group A （IL-6：1.86±0.21 vs 1.41 ±0.33, 1.38± 0.42, P 〈 0.01; TNF-α： 1.18±0.07 vs 0.66 ±0.08, 0.86 ±0.43, P 〈 0.01, P 〈 0.05）, whereas serum adiponectin and IL-10 decreased in model groups compared with that in the control （adiponectin： A： 21.87±4.84 and B： 21.48 ±4.60 vs 27.36 ±7.29, P 〈 0.05. IL-10： A： 1.72± 0.38 and B： 1.83 ±0.39 vs 2.26±0.24, P 〈 0.01）. Lg （TC） and the degree of liver fatty infiltration was an independent determinant of serum adiponectin level analyzed by stepwise multiple regressions, resulting in 29.4% of variances. CONCLUSION： This rabbit model produces the key features of pediatric NASH and may provide a realistic model for future studies. Adiponectin level partially reflects the severity of liver steatosis, but not the degree of liver inflammation.展开更多
AIM:To determine the associations between leptin and ghrelin concentrations and sustained virological response(SVR)in chronic hepatitis C patients with ste-atosis.METHODS:We retrospectively assessed 56 patients infect...AIM:To determine the associations between leptin and ghrelin concentrations and sustained virological response(SVR)in chronic hepatitis C patients with ste-atosis.METHODS:We retrospectively assessed 56 patients infected with hepatitis C virus(HCV)genotype-1 and 40 with HCV genotype-3.Patients with decompensated cirrhosis,and those with other causes of chronic liver disease,were excluded.Serum HCV-RNA concentra-tions were measured before the initiation of treatment;at weeks 12(for genotype 1 patients),24 and 48 during treatment;and 24 wk after the end of treatment.Genotype was determined using INNO-LIPA HCV as-says,and serum leptin and ghrelin concentrations were measured using enzyme-linked immunosorbent assay.Biopsy specimens were scored according to the Ishak system and steatosis was graded as mild,moderate,or severe,according to the Brunt classif ication.RESULTS:Overall,SVR was positively related to the presence of genotype-3,to biopsy-determined lower histological stage of liver disease,and lower grade of steatosis.Patients ≥ 40 years old tended to be less responsive to therapy.In genotype-1 infected pa-tients,SVR was associated with a lower grade of liver steatosis,milder fibrosis,and an absence of insulin resistance.Genotype-1 infected patients who did not achieve SVR had significantly higher leptin concen-trations at baseline,with significant increases as the severity of steatosis worsened,whereas those who achieved SVR had higher ghrelin concentrations.In genotype-3 infected patients,SVR was associated only with fibrosis stage and lower homeostasis model as-sessment insulin resistance at baseline,but not with the degree of steatosis or leptin concentrations.Geno-type-3 infected patients who achieved SVR showed signif icant decreases in ghrelin concentration at end of treatment.Baseline ghrelin concentrations were elevat-ed in responders of both genotypes who had moderate and severe steatosis.CONCLUSION:Increased serum leptin before treat-ment may predict non-SVR,especially in HCV geno-type-1 infected patients,whereas increased ghrelin may predict SVR in genotype-1.展开更多
Objective:To explore the serum levels of resistin and adiponectin in patients with overweight and obesity.Methods:Fifty-eight cases with normal weight and 24 patients with overweight and obesity have taken fasting blo...Objective:To explore the serum levels of resistin and adiponectin in patients with overweight and obesity.Methods:Fifty-eight cases with normal weight and 24 patients with overweight and obesity have taken fasting blood samples for measurements of plasma glucose,plasma lipids,insulin,C-peptide, thyroid hormones,C-response protein,interleukin-6,TNF-α,leptin,adiponectin and resistin.Results: The concentrations of resistin in cases with overweight and obesity were significant higher than those in the normal weight cases (16.01±8.60 vs 11.63±9.05ng/ml,P=0.047).Pearson relation analysis showed that serum resistin concentrations were positively correlated with age (r=0.476,P=0.019),but negatively correlated with C-peptide (r=-0.45,P=0.024),and adiponectin concentrations were posi- tively correlated with HDL-c (r=0.463,P=0.023) and systolic blood pressure (r=0.409,P=0.047)in overweight and obesity cases.Conclusion:The concentrations of resistin in cases with over-weight and obesity are higher,and there is no correlation between resistin and blood glucose,blood lipids and insulin, while the serum adiponectin concentrations positively correlated with HDL-c and systolic blood pressure.展开更多
In this research, Lysolecithin-a substance made with 100% natural ingredients - was given to ICR mice as medication to measure its periodic effect on the noradrenalin (NA), dopamine (DA), and serotonin (5-HT) levels o...In this research, Lysolecithin-a substance made with 100% natural ingredients - was given to ICR mice as medication to measure its periodic effect on the noradrenalin (NA), dopamine (DA), and serotonin (5-HT) levels of the brain. Both ICR and SAM mice were separated into two groups - control group and Lysolecithin (K. Lysolecithin: hydrolytic lysolecithin) medicated group, and given 1-week preparation period. The K. Lysolecithin group was given 500mg/kg of K. Lysolecithin at 0.2mL per dosage for 4 weeks, and the control group was given the same amount of dosage of water during the same period. NA, DA and 5-HT concentrations were measured from the blood before medication and 8 weeks / 12 weeks / 16 weeks after the first medication. For the SAM mice, 8 weeks after they were medicated with K .Lysolecithin, Morris Water Maze Test was conducted for 7 consecutive days and then the concentrations were measured by drawing blood from the heart. The K. Lysolecithin medicated group showed a tendency to have a statistically significant higher concentrations of 5-HT and NA in the blood. Also, periodic examination showed that the monoamine levels were highest in the 12th week and declined thereafter.展开更多
Objective To investigate the relationship between serum adiponectin levels with adiposity,glucocorticoids, insulin and leptin in Cushing’ s syndrome, obesity and non-obese subjects. Methods The serumadiponectin conce...Objective To investigate the relationship between serum adiponectin levels with adiposity,glucocorticoids, insulin and leptin in Cushing’ s syndrome, obesity and non-obese subjects. Methods The serumadiponectin concentrations were measured in 104 non-obese and 57 overweight or obese subjects byRIA. 15 patients with Cushing’ s syndrome, 10 with obesity and 9 non-obese subjects were investigated, with theirserum adiponectin, glucocorticoids, insulin and leptin levels measured at 8:00, 12:00, 16:00, 20:00, 24:00 and3:00. Dexamethasone suppression tests in both obesity and Cushing’s syndrome were performed at the dose of Img,2mg and 5mg. Results The serum adiponectin concentrations in non-obese were (10. 15 ±6. 33)mg/L in maleand (13. 82 ±6. 09)mg/L in female, and those in overweight or obese ones were (5. 78 ±3. 55)mg/L in male and(8. 13 ± 4. 32) mg/L in female. In both men and women, the fasting adiponectin levels in overweight or obese sub-jects were lower than those of the non-obese ones, and serum adiponectin concentrations were significantly nagetivelycorrelated with BMI, % Fat and waist circumference. The circadian rhythmicity of adiponectin was not distinct, butthe adiponectin levels in obesity were lower than those of the non-obese subjects at all 6 time spots. The serum adi-ponectin area under curve (AUC) were significantly nagetively correlated with BMI, waist circumference and insulinA UC. The adiponectin levels with dexamethasone administration for a short-term both at higher doses and lower do-ses did not change, but was decreased after surgery. Conclusion Adiponectin is a hormone secreted by adipo-cytes which may intimately related to obese and insulin resistance. Therefore, any treatment that could be used to in-crease adiponectin should be beneficial. Neither long-term endogenous hyper-glucocorticoid nor short-term dexam-ethasone administration may affect the adiponectin levels, and similarly, no change with elevated postprandial insu-lin levels.展开更多
基金Supported by The funds for programs of Zhejiang Provincial Natural Science, No.Y2080047Major Programs of Zhejiang Provincial Medical and Health Science and Technology & Chinese Ministry of Health, No.WKJ2008-2-026Special Major Programs of Zhejiang Provincial Science and Technology, No. 2008c03002-1
文摘AIM： To create a rabbit model of pediatric nonalcoholic steatohepatitis （NASH） and to evaluate the role of adiponectin in the process. METHODS： Thirty-two specific pathogen-free male New Zealand rabbits were divided randomly into three groups： （1） the normal control group （n = 10） was fed with standard diet for 12 wk; （2） the model group A （n = 11）; and （3） model group B （n = 11） were fed with a highfat diet （standard diet ＋ 10% lard ＋ 2% cholesterol） for 8 and 12 wk, respectively. Hepatic histological changes were observed and biochemical parameters as well as serum levels of adiponectin, interleukin （IL）-6, IL-10 and tumor necrosis factor （TNF）-α were measured. RESULTS： Typical histological hepatic lesions of NASH were observed in both model groups described as liver steatosis, liver inflammatory infiltration, cytologic ballooning, perisinusoidal fibrosis and overall fibrosis. Compared with the normal control group, there were significant increases in model groups A and B in weight gain （1097.2 ± 72.3, 1360.5± 107.6 vs 928.0 ±58.1, P 〈 0.05, P 〈 0.01）, liver weight （93.81±6.64, 104.6±4.42 vs 54.4±1.71, P 〈 0.01）, Lg （ALT） （1.9±0.29, 1.84± 0.28 vs 1.60±0.17, P 〈 0.01）, and Lg （TG） （1.03 ±0.24, 1.16 ±0.33 vs 0.00 ±0.16, P 〈 0.01）. Weight gain was much more in model group B than in model group A （1360.5± 107.6 vs 1097.2 ±72.3, P 〈 0.05）. But, there was no significant difference between the two groups concerning the other indexes. Pro-inflammatory cytokines （IL-6 and TNF-α） increased in model group B compared with that of control and model group A （IL-6：1.86±0.21 vs 1.41 ±0.33, 1.38± 0.42, P 〈 0.01; TNF-α： 1.18±0.07 vs 0.66 ±0.08, 0.86 ±0.43, P 〈 0.01, P 〈 0.05）, whereas serum adiponectin and IL-10 decreased in model groups compared with that in the control （adiponectin： A： 21.87±4.84 and B： 21.48 ±4.60 vs 27.36 ±7.29, P 〈 0.05. IL-10： A： 1.72± 0.38 and B： 1.83 ±0.39 vs 2.26±0.24, P 〈 0.01）. Lg （TC） and the degree of liver fatty infiltration was an independent determinant of serum adiponectin level analyzed by stepwise multiple regressions, resulting in 29.4% of variances. CONCLUSION： This rabbit model produces the key features of pediatric NASH and may provide a realistic model for future studies. Adiponectin level partially reflects the severity of liver steatosis, but not the degree of liver inflammation.
文摘AIM:To determine the associations between leptin and ghrelin concentrations and sustained virological response(SVR)in chronic hepatitis C patients with ste-atosis.METHODS:We retrospectively assessed 56 patients infected with hepatitis C virus(HCV)genotype-1 and 40 with HCV genotype-3.Patients with decompensated cirrhosis,and those with other causes of chronic liver disease,were excluded.Serum HCV-RNA concentra-tions were measured before the initiation of treatment;at weeks 12(for genotype 1 patients),24 and 48 during treatment;and 24 wk after the end of treatment.Genotype was determined using INNO-LIPA HCV as-says,and serum leptin and ghrelin concentrations were measured using enzyme-linked immunosorbent assay.Biopsy specimens were scored according to the Ishak system and steatosis was graded as mild,moderate,or severe,according to the Brunt classif ication.RESULTS:Overall,SVR was positively related to the presence of genotype-3,to biopsy-determined lower histological stage of liver disease,and lower grade of steatosis.Patients ≥ 40 years old tended to be less responsive to therapy.In genotype-1 infected pa-tients,SVR was associated with a lower grade of liver steatosis,milder fibrosis,and an absence of insulin resistance.Genotype-1 infected patients who did not achieve SVR had significantly higher leptin concen-trations at baseline,with significant increases as the severity of steatosis worsened,whereas those who achieved SVR had higher ghrelin concentrations.In genotype-3 infected patients,SVR was associated only with fibrosis stage and lower homeostasis model as-sessment insulin resistance at baseline,but not with the degree of steatosis or leptin concentrations.Geno-type-3 infected patients who achieved SVR showed signif icant decreases in ghrelin concentration at end of treatment.Baseline ghrelin concentrations were elevat-ed in responders of both genotypes who had moderate and severe steatosis.CONCLUSION:Increased serum leptin before treat-ment may predict non-SVR,especially in HCV geno-type-1 infected patients,whereas increased ghrelin may predict SVR in genotype-1.
文摘Objective:To explore the serum levels of resistin and adiponectin in patients with overweight and obesity.Methods:Fifty-eight cases with normal weight and 24 patients with overweight and obesity have taken fasting blood samples for measurements of plasma glucose,plasma lipids,insulin,C-peptide, thyroid hormones,C-response protein,interleukin-6,TNF-α,leptin,adiponectin and resistin.Results: The concentrations of resistin in cases with overweight and obesity were significant higher than those in the normal weight cases (16.01±8.60 vs 11.63±9.05ng/ml,P=0.047).Pearson relation analysis showed that serum resistin concentrations were positively correlated with age (r=0.476,P=0.019),but negatively correlated with C-peptide (r=-0.45,P=0.024),and adiponectin concentrations were posi- tively correlated with HDL-c (r=0.463,P=0.023) and systolic blood pressure (r=0.409,P=0.047)in overweight and obesity cases.Conclusion:The concentrations of resistin in cases with over-weight and obesity are higher,and there is no correlation between resistin and blood glucose,blood lipids and insulin, while the serum adiponectin concentrations positively correlated with HDL-c and systolic blood pressure.
文摘In this research, Lysolecithin-a substance made with 100% natural ingredients - was given to ICR mice as medication to measure its periodic effect on the noradrenalin (NA), dopamine (DA), and serotonin (5-HT) levels of the brain. Both ICR and SAM mice were separated into two groups - control group and Lysolecithin (K. Lysolecithin: hydrolytic lysolecithin) medicated group, and given 1-week preparation period. The K. Lysolecithin group was given 500mg/kg of K. Lysolecithin at 0.2mL per dosage for 4 weeks, and the control group was given the same amount of dosage of water during the same period. NA, DA and 5-HT concentrations were measured from the blood before medication and 8 weeks / 12 weeks / 16 weeks after the first medication. For the SAM mice, 8 weeks after they were medicated with K .Lysolecithin, Morris Water Maze Test was conducted for 7 consecutive days and then the concentrations were measured by drawing blood from the heart. The K. Lysolecithin medicated group showed a tendency to have a statistically significant higher concentrations of 5-HT and NA in the blood. Also, periodic examination showed that the monoamine levels were highest in the 12th week and declined thereafter.
文摘Objective To investigate the relationship between serum adiponectin levels with adiposity,glucocorticoids, insulin and leptin in Cushing’ s syndrome, obesity and non-obese subjects. Methods The serumadiponectin concentrations were measured in 104 non-obese and 57 overweight or obese subjects byRIA. 15 patients with Cushing’ s syndrome, 10 with obesity and 9 non-obese subjects were investigated, with theirserum adiponectin, glucocorticoids, insulin and leptin levels measured at 8:00, 12:00, 16:00, 20:00, 24:00 and3:00. Dexamethasone suppression tests in both obesity and Cushing’s syndrome were performed at the dose of Img,2mg and 5mg. Results The serum adiponectin concentrations in non-obese were (10. 15 ±6. 33)mg/L in maleand (13. 82 ±6. 09)mg/L in female, and those in overweight or obese ones were (5. 78 ±3. 55)mg/L in male and(8. 13 ± 4. 32) mg/L in female. In both men and women, the fasting adiponectin levels in overweight or obese sub-jects were lower than those of the non-obese ones, and serum adiponectin concentrations were significantly nagetivelycorrelated with BMI, % Fat and waist circumference. The circadian rhythmicity of adiponectin was not distinct, butthe adiponectin levels in obesity were lower than those of the non-obese subjects at all 6 time spots. The serum adi-ponectin area under curve (AUC) were significantly nagetively correlated with BMI, waist circumference and insulinA UC. The adiponectin levels with dexamethasone administration for a short-term both at higher doses and lower do-ses did not change, but was decreased after surgery. Conclusion Adiponectin is a hormone secreted by adipo-cytes which may intimately related to obese and insulin resistance. Therefore, any treatment that could be used to in-crease adiponectin should be beneficial. Neither long-term endogenous hyper-glucocorticoid nor short-term dexam-ethasone administration may affect the adiponectin levels, and similarly, no change with elevated postprandial insu-lin levels.
