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高效液相色谱法测定姜黄素清蛋白纳米粒中药物含量与包封率 被引量:2
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作者 邓俊刚 马林 +1 位作者 杨春晓 刘亚妮 《医药导报》 CAS 北大核心 2013年第7期936-938,共3页
目的建立测定姜黄素清蛋白纳米粒中主药含量及包封率的方法。方法采用高效液相色谱法,色谱柱为Hypersil C18柱(250 mm×4.6 mm,5μm);流动相:乙腈-4%冰醋酸(55∶45);流速:1.0 mL.min-1;柱温:30℃;检测波长:425 nm;进样量:20μL;采... 目的建立测定姜黄素清蛋白纳米粒中主药含量及包封率的方法。方法采用高效液相色谱法,色谱柱为Hypersil C18柱(250 mm×4.6 mm,5μm);流动相:乙腈-4%冰醋酸(55∶45);流速:1.0 mL.min-1;柱温:30℃;检测波长:425 nm;进样量:20μL;采用低温超速离心法分离姜黄素清蛋白纳米粒和游离药物,测定包封率。结果姜黄素在0.102~16.32μg.mL-1浓度范围内线性关系良好(r=0.999 9),平均回收率为(99.96±1.01)%(n=9),以标示量计平均含量为(100.31±0.94)%,平均包封率为(71.13±1.30)%。结论该方法灵敏、简便、准确,适用于姜黄素清蛋白纳米粒药物含量及包封率的测定。 展开更多
关键词 姜黄素 清蛋白纳米粒 含量测定 包封率 色谱法 高效液相
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Roles of Biomolecules in the Biosynthesis of Silver Nanoparticles: Case of Gardenia jasminoides Extract
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作者 吕芬芬 高艺 +2 位作者 黄加乐 孙道华 李清彪 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2014年第6期706-712,共7页
Rapid development of biosynthesis of metal nanoparticles using plants has attracted extensive interests to further investigate this novel and eco-friendly method. In the biosynthesis process, the plant may act as redu... Rapid development of biosynthesis of metal nanoparticles using plants has attracted extensive interests to further investigate this novel and eco-friendly method. In the biosynthesis process, the plant may act as reducing agent, capping agent or shape directing agent. However, identifying specific roles of various components in the plant is challenging. In this study, we use biosynthesis of silver nanoparticles with Gardenia jasminoides Ellis extract to address this issue. The formation process of silver nanoparticles is investigated and the nanoparticles are characterized with the ultraviolet-visible spectroscopy, Fourier transform infrared spectra and scanning electron microscopy. The results indicate that the Gardenia jasminoides Ellis extract can reduce silver ions to form silver nanoparticles, stabilize the nanoparticles, and affect the growth of silver nanocrystal to form silver nanowires. Only geniposide in the extract exhibits good shape-directing ability for silver nanowires. It is found that bovine albumin is a potential capping agent, whereas rutin, gallic acid and chlorogenic acid possess reducing and capping ability. 展开更多
关键词 BIOSYNTHESIS NANOPARTICLE NANOWIRE silver
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