逆转录PCR扩增杂交瘤细胞内特异性免疫球蛋白重链可变区基因

目的 利用细胞内逆转录PCR技术扩增杂交瘤细胞内的特异免疫球蛋白重链可变区基因片段。方法 用 10 %甲醛溶液固定杂交瘤细胞 ,以NP 40渗透化处理细胞后做逆转录PCR。结果 获得了大约 3 5 0bp的特异性免疫球蛋白重链可变区基因片段 ,与用同一对引物得到的常规逆转录PCR扩增产物一致。结论 此方法操作简便 ,效率高 ,可用于获得特定结构基因片段 。

Pretreatment of Isopropanol Solution from Pharmaceutical Industry and Pervaporation Dehydration by NaA Zeolite Membranes

NaA zeolite membranes with 80 cm in length and 12.8 mm in outer diameter were prepared by our research group cooperating with Nanjing Jiusi Hi-Tech Co., China. The influence of dissolved inorganic salts and pH value in the feed of isopropanol （IPA） solution on NaA zeolite membranes was investigated. It was found that both factors exhibited strong influence on the stability of NaA zeolite membranes. A set of pretreatment steps such as pH adjustment and distillation of the IPA solution were proposed to improve stability for pervaporation dehydration. An industrial-scale pervaporation facility with 52 m2 membrane area was built to dehydrate IPA solution from industrial cephalosporin production. The facility was continuously operated at 368-378 K to dehydrate IPA solution from water mass content of 15%-20% to less than 2% with a feed flow rate of 400-500 L·h^-1 and an average water flux of 1-1.5 kg·m^-2·h-1. The successful application of this facility suggested a promising application of NaA zeolite mem-brane for IPA recovery from pharmaceutical production.

Effects of Chemical Osmotic Pretreatment on Drying Characteristics and Quality of Blueberry under Hot Air Drying

In order to study the effects of chemical osmotic pretreatment on the characteristics and quality of blueberry under hot air drying,fresh blueberries were pretreated with 2.5 g/100 mL K2CO3+0.6 g/100 mL olive oil,and 5.0 g/100 mL K2CO3+0.6 g/100 mL olive oil at(45±0.5)℃,respectively.The changes of water content,rehydration,hardness,microstructure,color difference,active ingredient anthocyanin,total phenol and DPPH radical scavenging capacity of dried blueberries in different treatment groups under hot air drying were compared and analyzed.The results showed that the dehydration rates of blueberries vary greatly according to the type of pretreatments when the samples were dried to the same water content with hot air.Specifically,the dehydration rate of dried blueberries pretreated by 5.0%K2CO3 solution was the highest,followed by 2.5%K2CO3 osmotic pretreatment and lastly the control group;the corresponding dehydration time was 10,14 and 20h,respectively.The physical qualities of dried blueberries,involving the browning degree,color difference,rehydration and microstructure,were significantly different between the chemical osmotic pretreatment group and the control group(P<0.05).The chemical osmotic pretreatment of K2CO3 solution increased the dehydration rate of the samples,shortened the drying time and maintained the quality of blueberries dried with hot air.There was no significant difference between the physical quality of dried blueberries pretreated by 2.5%and 5.0%K2CO3 solution(P>0.05),whereas there was significant difference in drying time and nutrient quality which is characterized by total phenols,anthocyanins,DPPH radical scavenging rate,soluble total sugar(P<0.05).Conclusion:5.0%K2CO3 osmotic pretreatment combining with hot air drying can improve the dehydration rate,shorten the drying time and maintain the physical and nutritional quality.

Nanometallocarbosilanes： Synthesis, Physicochemical Properties and Structure

Highly efficient synthesis methods have been developed and characteristics of nanometallocarbosilanes molecular structure were studied by the research team of GNIIChTEOS （State Research Institute for Chemistry and Technology of Organoelement Compounds）. Nanometallocarbosilanes were synthesized by thermal co-condensation of oligocarbosilanes and alkyl amides of refractory metals. Initial, intermediate and final products of side reactions were characterized by 1H, 13C, 29Si NMR （nuclear magnetic resonance）, IR （infra-red） spectroscopy, GPC （gel-penetrating chromatography）, TGA （thermal gravimetric analysis）, TEM （transmission electron microscopy）, SEM （scanning electron microscopy）, RES （X-ray phase analysis） and elemental analysis. The proposed synthesis method of nanometallocarbosilanes was lbund to produce fusable soluble organosilicon oligomers with homogeneous distribution of nanoscale （10-20 nm） metal particles in the oligomer matrix. A computational model of the group and elemental composition of nanometallocarbosilanes was developed; it was shown that they are molecular globules of near-spherical shape and rigid polycyclic structure. Thermochemical treatment of nanometallocarbosilanes leads to SiC-nanoceramics （a high yield of up to 75-80 mass%） modified by metal nanoparticles （20-30 nm） contributing to its stabilization. The application of preceramic oxygen-free nanometallocarbosilanes will make it possible to advance in solving the problem of ceramic composite materials with long-term resistance at temperatures above 1,500 ℃ in oxidizing environments.

不同浓度链球菌溶血素O对小鼠胎儿成纤维细胞生长状态的影响

本研究比较了0、10、25、50 U或100 U链球菌溶血素O(SLO)处理下的小鼠胎儿成纤维细胞(MEFs)的细胞密度、存活率、贴壁率,并绘制了适宜浓度SLO下MEFs的生长曲线。结果表明:25 U SLO处理组的细胞密度与10 U SLO处理组间差异不显著(P>0.05),但极显著高于50 U和100 U SLO处理组(P<0.01)。0、10 U和25 USLO处理组的细胞存活率差异不显著(P>0.05),但显著高于50 U处理组(P<0.05)。25 U SLO处理组的贴壁率极显著高于50 U和100 U SLO处理组(P<0.01)。25 U SLO处理组MEFs具有正常分裂增殖生长模式。因此,为了既能达到SLO对MEFs细胞膜的渗透化效果,又尽可能减少对细胞的损伤,宜选用25 U SLO进行MEFs的渗透化处理。