Ramie (Boehmeria nivea L.) is one of the most important fiber crops and biomass materials. However, previous studies showed that ramie presented a very low nitrogen agronomy efficiency (NAE, 23.2%~27.8%) in traditiona...Ramie (Boehmeria nivea L.) is one of the most important fiber crops and biomass materials. However, previous studies showed that ramie presented a very low nitrogen agronomy efficiency (NAE, 23.2%~27.8%) in traditional farming, and the nitrogen fertilizer was applied excessively in ramie field. Plant osmotic adjustment (OA) responses to environmental stresses positively and exhibits improvements in plant tolerance. Whereas results varied due to the complexity of plant-environment interactions and lack of insights of specific species. In order to improve ramie production through osmoregulation, our current study investigated the role of nitrogen application and osmotic adjustment in improving the growth and yield in two varieties of ramie (H2000-03 and Ceheng Jiama) with contrasting nitrogen use efficiency (NUE) grown at 5 different N rates including N0, N6, N9, N12 and N15;0, 6, 9, 12 and 15 mmol/L N, respectively. The results showed that ramie adapted to different nitrogen rates through OA and significant differences of osmolyte content between varieties only presented at the particular growth stage. Obvious inflexion of yield, osmolyte content involving proline, soluble protein (SP), soluble sugar (SS) and malonaldehyde (MDA);nitrogen sensitive index (NSI) and comprehensive evaluation (D) in both varieties were observed. Our results recommended that the overall lifting of OA at a lower N level and at the proper growth stage would be a reasonable approach for improving ramie NUE.展开更多
An 8 weeks feeding experiment was conducted to determine the effect of dietary potassium on the growth and physio-logical acclimation of Pacific white shrimp (Litopenaeus vannamei) reared in diluted seawater (salin...An 8 weeks feeding experiment was conducted to determine the effect of dietary potassium on the growth and physio-logical acclimation of Pacific white shrimp (Litopenaeus vannamei) reared in diluted seawater (salinity 4). Six semi-purified practical diets containing 0.59, 0.96, 1.26, 1.48, 1.74, and 2.17 g potassium K+ per 100 g diet were formulated, respectively. The survival and feed conversion rate did not show significant difference among groups of shrimps given these diets (P〉0.05). The shrimps fed the diets containing 0.96-1.48 g K+ per 100g diet gained the highest weight, specific growth rate, and protein efficiency ratio. Their ammonium-N excretion rate as well as hemolymph concentration of Na+ and C1- were significantly lower than those of the control (P〈0.05), but a reverse trend was observed for their gill Na+/K+-ATPase. Moreover, the shrimps fed with 1.48 g K+ per 100 g diet were the highest in hemolymph urea level, and the phenoloxidase and lysozyme activities were significantly higher than those of the control (P〈0.05). The growth and physiological response of the test shrimps suggested that diet containing 1.48 g K+ per 100 g diet improved the growth of L. vannamei in low-salinity seawater, and enhanced the physiological acclimation of the organism.展开更多
文摘Ramie (Boehmeria nivea L.) is one of the most important fiber crops and biomass materials. However, previous studies showed that ramie presented a very low nitrogen agronomy efficiency (NAE, 23.2%~27.8%) in traditional farming, and the nitrogen fertilizer was applied excessively in ramie field. Plant osmotic adjustment (OA) responses to environmental stresses positively and exhibits improvements in plant tolerance. Whereas results varied due to the complexity of plant-environment interactions and lack of insights of specific species. In order to improve ramie production through osmoregulation, our current study investigated the role of nitrogen application and osmotic adjustment in improving the growth and yield in two varieties of ramie (H2000-03 and Ceheng Jiama) with contrasting nitrogen use efficiency (NUE) grown at 5 different N rates including N0, N6, N9, N12 and N15;0, 6, 9, 12 and 15 mmol/L N, respectively. The results showed that ramie adapted to different nitrogen rates through OA and significant differences of osmolyte content between varieties only presented at the particular growth stage. Obvious inflexion of yield, osmolyte content involving proline, soluble protein (SP), soluble sugar (SS) and malonaldehyde (MDA);nitrogen sensitive index (NSI) and comprehensive evaluation (D) in both varieties were observed. Our results recommended that the overall lifting of OA at a lower N level and at the proper growth stage would be a reasonable approach for improving ramie NUE.
文摘An 8 weeks feeding experiment was conducted to determine the effect of dietary potassium on the growth and physio-logical acclimation of Pacific white shrimp (Litopenaeus vannamei) reared in diluted seawater (salinity 4). Six semi-purified practical diets containing 0.59, 0.96, 1.26, 1.48, 1.74, and 2.17 g potassium K+ per 100 g diet were formulated, respectively. The survival and feed conversion rate did not show significant difference among groups of shrimps given these diets (P〉0.05). The shrimps fed the diets containing 0.96-1.48 g K+ per 100g diet gained the highest weight, specific growth rate, and protein efficiency ratio. Their ammonium-N excretion rate as well as hemolymph concentration of Na+ and C1- were significantly lower than those of the control (P〈0.05), but a reverse trend was observed for their gill Na+/K+-ATPase. Moreover, the shrimps fed with 1.48 g K+ per 100 g diet were the highest in hemolymph urea level, and the phenoloxidase and lysozyme activities were significantly higher than those of the control (P〈0.05). The growth and physiological response of the test shrimps suggested that diet containing 1.48 g K+ per 100 g diet improved the growth of L. vannamei in low-salinity seawater, and enhanced the physiological acclimation of the organism.