水稻白条纹叶及白穗突变体wlp6的鉴定与基因定位

【目的】叶色突变体是研究水稻光合作用,叶绿素生物合成和遗传发育调控机理的重要材料。发掘水稻叶色突变体,是水稻功能基因组学研究的重要遗传基础。【方法】在昌恢121中发现了一份白条纹叶及抽穗期白穗突变体,经过连续多代自交能稳定遗传,暂命名为wlp6(white striped leaf and white panicle 6)。在南昌分早、中和晚3季播种wlp6与野生型种子,考查了中稻与晚稻的部分农艺性状;测定3叶期、分蘖期、抽穗期叶片及颖壳的叶绿素含量;通过电镜观察抽穗期叶肉细胞发育情况。在光照培养箱中进行温光敏感实验;将wlp6与昌恢121及02428正反交,观察F_1植株表型,对F_2分离群体进行卡方测验,分析突变体遗传规律;以wlp6/02428衍生的F_2群体为材料,利用BSA法进行基因定位。【结果】wlp6自第1片叶到成熟,叶片均呈白条纹,抽穗期颖壳及枝梗失绿,高温天气穗转绿。突变体株高、有效穗数和每穗粒数在早稻季和中稻季均显著低于野生型,晚稻季wlp6的结实率和千粒重也显著低降低。叶绿素含量测定表明,wlp6叶片叶绿素含量在不同生育期及不同季均显著低于野生型,早稻和晚稻季种植的wlp6颖壳叶绿素含量也比野生型低。电镜观察抽穗期的叶肉细胞发现,wlp6叶绿体数目减少,体积变小,没有分化出明显的片层结构。温光敏感实验表明,突变体对光照强弱钝感,叶色受温度和日照长短影响,随着温度升高和日照时间变长突变体叶绿素含量有上升趋势。遗传分析表明,该性状受隐性核基因控制,利用wlp6/02428得到的616个F_2单株将WLP6定位于第6染色体短臂InDel标记R-7与R-8间,物理距离137kb,此区间预测了21个候选基因。经候选基因分析及测序发现,其中LOC_Os06g14620编码一个核糖核酸还原酶小亚基,编码区第142和158位碱基由T替换为C,第288位插入了碱基A,碱基的插入导致翻译提前终止,因此推测LOC_Os06g14620是WLP6的候选基因。【结论】LOC_Os06g14620是已经克隆的白条纹叶基因St1的候选基因,推测WLP6与St1等位,但突变位点不同,且表型也有差异。

优质型杂交粳稻“北种南制”的策略

针对高食味优质型粳稻常规品种和以其为亲本转育的不育系(含辽粳433系、越光系和港源系血缘)南北跨区域种植的差异化表现,探讨该类型杂交组合"北种南制"的可行性,分析生育期"倒挂"现象产生的原因,并概括总结适合南繁稻区的配套制种技术,为优质型杂交粳稻的高产、高效、安全制种提供理论依据。

Effect of High Temperature on Outcrossing Characteristics at the Fertility Sensitive Period of Photo-thermo Sensitive Genic Male Sterile (PTGMS) Rice Lines

Four sterile lines （Peiai64S, Y58S, Guangzhan 63-2S and H638S） and the restoring line R527 were used as materials. Five temperature gradients （24, 27, 30, 33 and 36 ℃ in artificial climate chamber） and the natural temperature （as control） were treated to the four sterile lines for 6 d in the fertility sensitive period of heading to flowering stage, respectively, to study the effects of temperature on physiological biochemical characteristics of young panicles and outcrossing characteristics. The results showed that the percentages of exerted stigma of Peiai 64S and Y58S were the highest at 27 ℃, which were 86.81% and 86.06%, respectively, while the percentages of exerted stigma of Guangzhan 63-2S and H638S were the highest at 24 ℃, which were 76.24% and 81.76%, respectively; the stigma viability of Peiai 64S, Y58S, Guangzhan 63-2S and H638S were the best at 24 ℃, which were 1.96, 2.12, 1.74 and 1.94, respectively; the outcrossing rates of Peiai 64S, Guangzhan 63-2S and H638S were the highest at 24 ℃, which were 58.87%, 54.22% and 50.50%, respectively, while the outcrossing rate of Y58S was the highest at 27 ℃, which was 58.96%; and the contents of peroxidase （POD） of the four sterile lines increased significantly at 33 ℃ compared with the control, while the contents of malondialdehyde （MDA） and proline increased significantly at 36 ℃ compared with the control. There were differences in temperature sensitivity between the male sterile lines, and the 24 ℃ treatment during the sensitive period was the best for the fertility sensitive period of Peiai 64S, while 27 ℃ was the best temperature for Y58S, Gangzhan 63-2S and H638S.

Optical-fiber distributed temperature sensor:design and realization

Through analyzing theoretically the temperature effect of the optical-fiber Raman backscattering,a distributed temperature sensor is designed based on the single-mode fiber. Demodulation methods of temperature transduction are compared,and then the demodulation method using the ratio of the anti-Stokes and the Stokes Raman backscattering intensity is adopted. Both the hardware composition and the software realization of the system are introduced in detail. The experiment shows that the distinguishing ability of the temperature and that of the space are 1℃ and 2m,respectively,and that the system response time is about 180 s with a sensing range of 5km and with a temperature measurement range of 0-100℃.

High-sensitivity temperature sensor based on long-period fiber grating

Temperature sensitivity is greatly improved by taking the following three measures： proper long-period fiber grating （LPFG） whose strain coefficient of the core is larger than that of the cladding is employed, the LPFG is coated with a thin film of the material whose refractive index decreases with the temperature, and the sensor is encapsulated by metal material whose thermal expansion coefficient is large. By computer simulation, a measured temperature coefficient of 0.2375 nm/℃ and a temperature resolution less than 0.1 ℃ are obtained.

FBS Effect and Temperature Dependence in Trench-Assisted Multimode Fiber

We propose the trench-assisted multimode fiber(TA-OM4)as a novel sensing fiber in forward Brillouin scattering(FBS)-based temperature sensor,due to its higher temperature sensitivity,better bending resistance and lower propagation loss,compared with the single mode fiber(SMF)and other sensing fibers.The FBS effect and acousto-optic interaction in TA-OM4 are the first time to be demonstrated and characterized at 1550 nm theoretically and experimentally.A 2.0 km long TA-OM4 is put into an oven to measure its temperature sensitivity,which can reach up to 80.3 kHz/℃,exceeding 53%of SMF(52.4 kHz/℃).The simulated and experimental results verify that the TA-OM4 may be a good candidate as the sensing fiber for the FBS-based temperature sensor.

Method of correlation function for analyzing cross-sensitivity of strain and temperature in fiber grating sensors

A novel method of correlation function for analyzing cross-sensitivity between strain and temperature is reported for the first time in this paper. Using the new method,the correlative characteristics between strain and temperature of fiber Bragg grating sensors are studied both theoretically and experimentally The experimental results accord with the theoretical calculations.

Detection of Oxygen Based on Host-Guest Doped Room-Temperature Phosphorescence Material

Quantitative oxygen detection,especially at low concentrations,holds significant importance in the realms of biology,complex environments,and chemical process engineering.Due to the high sensitivity and rapid response of the triplet excitons of phosphorescence to oxygen,pure organic room-temperature phosphorescence(RTP)materials have garnered widespread attention in recent years for oxygen detection.However,simultaneously achieving ultralong phosphorescence at room temperature and quantitative oxygen detection from pure organic host-guest doped materials poses challenges.The d ensely packed materials may decrease non-radiative decay to increase the phosphorescence,but are unsuitable for oxygen diffusion in oxygen detection.Herein,the oxygen sensitivity of host-guest doped RTP materials using 4-bromo-N,N-bis(4-(tertbutyl)phenyl)aniline(TPABuBr)as the host and 6-bromo-2-butyl-1H-benzo[de]isoquinoline-1,3(2H)-dione(NIBr)as the guest was developed.The doped material exhibits fluorescence-phosphorescence dual-emission behavior at room temperature.The tert-butyl groups in TPABuBr facilitate appropriate intermolecular spacing in the crystal state,enhancing oxygen permeability.Therefore,oxygen penetration can quench the phosphorescence emission.The observed linear relationship between the phosphorescence intensity of the doped material and the oxygen volume fraction conforms to the Stern-Volmer equation,suggesting its potential for quantitative analysis of oxygen concentration.The calculated limit of detection is 0.015%(φ),enabling the analysis of oxygen with a volume fraction of less than 2.5%(φ).Moreover,the doped materials demonstrate rapid response and excellent photostability,indicating their potential utility as oxygen sensors.This study elucidates the design and characteristics of NIBr/TPABuBr doped materials,highlighting their potential application in oxygen concentration detection and offering insights for the design of oxygen sensors.

Temperature-insensitive fiber Bragg grating strain sensor

A fiber Bragg grating strain sensor,whose reflection bandwidth is insensitive to temperature,is presented.The cross-sec-tional area is designed to change linearly.Under axial stress,there is a linear relationship between stress and average strain.Experimental results show that when temperature increases,reflection center wavelength shifts to longer wavelength,and there is a good linear relationship between center wavelength and temperature.When stress increases,reflection center wavelength shifts to longer wavelength,and reflection bandwidth increases.There are good linear relationships between reflection center wavelength and stress as well as reflection bandwidth and stress.

Simultaneous measurement of temperature and refractive index based on a core-offset Mach-Zehnder interferometer cascaded with a long-period fiber grating

An all-fiber sensor based on a cascaded optical fiber device is proposed and demonstrated, and its sensor head is composed of a core-offset Mach-Zehnder interferometer(MZI) and a long-period fiber grating(LPFG). In the experiment, two dips shaped by the intermodulation between the interference fringe of MZI and the resonant wavelength of LPFG are monitored. Experimental results show that temperature sensitivities of two dips are 0.060 7 nm/°C and 0.056 3 pm/°C, and the refractive index(RI) sensitivities are –18.025 nm/RIU and –55.06 nm/RIU, respectively. The simultaneous measurement of the temperature and external RI is demonstrated based on the sensitive matrix. Its low fabrication cost, simple configuration and high sensitivity make this sensor have potential applications in chemical and biological sensing.

Simultaneous sensing of displacement and temperature with a single FBG

A novel fiber Bragg grating(FBG) sensor with simultaneous sensing of displacement and temperature is presented.The FBG is affixed on the cantilever inclinedly.The midpoint of FBG exactly coincides with the zero strain layer of a rectangular beam.The vertical displacement can be measured by the broadened bandwidth of FBG as the bandwidth is insensitive to temperature,while the temperature can be measured by the center wavelength shift as the wavelength shift is insensitive to vertical displacement.With 0.1 nm spectral resolution of the analyzer,sensitivities of bandwidth-displacement and center wavelength-temperature are 0.48 nm/mm and 0.05 nm/℃,resolutions are 0.2 mm and 2.0 ℃,and sensing ranges of displacement and temperature are up to 8.5 mm and 45℃ respectively.Experimental results match theoretical analyses very well.

Micro-Structured Fiber Interferometer as Sensitive Temperature Sensor

We report on a fast and sensitive temperature sensor using a micro-structured or photonic crystal fiber interferometer with a high germanium doped fiber core. The wavelength sensitivity for temperature variation was as high as △λ/△T= 78 pm/℃ up to 500℃, which was 6 times more sensitive than the fiber Bragg grating temperature sensitivity of △λ/△TT= 13pm/℃ at 1550nm. The sensor device was investigated conceming the sensitivity characteristics and response time.

Study of Optical Fibers Strain-Temperature Sensitivities Using Hybrid Brillouin-Rayleigh System

In this paper, the most recent progress as well as challenges of distributed optical fiber sensing （DOFS） in industrial applications is discussed. Compared to the vast market of sensors used to measure strain or temperature, the success of distributed optical fiber sensing （DOFS） at the industrial level is very limited, at best. One of the reasons for this lack of the wider acceptance is the mismatch between the commercially available systems and actual industrial requirements, especially for the spatial resolution and precision. These requirements are organized and clarified in the paper. It also describes the hybrid Brillouin-Rayleigh system, which exhibits capabilities surpassing those of strain gauges. The principles of the system are illustrated considering the fiber calibration methodology. Formulas required for determining strain, temperature, and hydro-pressure are derived and discussed. Finally, the examples of applications are presented.

Temperature Insensitive Bending Sensor Based on In-Line Mach-Zehnder Interferometer

A simple and compact fiber bending sensor based on the Maeh-Zehnder interferometer was proposed. A photonic crystal fiber （PCF） with a length of 10mm was spliced by collapsing air holes with two conventional single mode fibers to consist of an all fiber bending sensor. The sensitivity of 0.53nm/m-1 was obtained at 1586nm for the curvature range from 0 to 8.514 m-1. The temperature sensitivity was very low. The measurement error due to the temperature effect was about 8.68x10-3 m-1/℃, and the temperature effect in the curvature measurement could be ignored. This device can avoid the cross sensitivity of the temperature in the curvature measurement.

Non-stoichiometry in Ca2Al2SiO7 enabling mixedvalent europium toward ratiometric temperature sensing

Eu^2+/Eu^3+ mixed-valence couple co-doped material holds great potential for ratiometric temperature sensing owing to its different electronic configurations and electron-lattice interaction. Here, the correlation of nonstoichiometry in chemical composition, phase structures and luminescence propertis of Ca2 Al2 Si1-xO7:Eu is discussed, and controlled Eu^2+/Eu^3+ valence and tunable emission appear with decreasing Si content. It is found that the 2 Ca^2++ Si^4+←→ Eu^2++ Eu^3++ Al^3+ cosubstitution accounts for the structural stability and charge balance mechanism. Benefiting from the diverse thermal dependent emission behaviors of Eu^2+ and Eu^3+, Ca2 Al2 Si1-xO7:Eu thermometer exhibits excellent temperature sensing performances with the maximum absolute and relative sensitivity being 0.024 K-1(at 303 K) and 2.46% K-1(at 443 K) and good signal discriminability. We propose that the emission quenching of Eu^2+ is ascribed to 5 d electrons depopulation through Eu^2+/Eu^3+ intervalence charge transfer state, while the quenching of Eu^3+ comes from multiphonon relaxation. Our work demonstrates the potential of Ca2 Al2 Si1-xO7:Eu for noncontact optical thermometry, and also highlights mixed-valence europium-containing compounds toward temperature sensing.

Magnetic field sensor based on peanut-shape structure and multimode fiber

An all fiber magnetic field sensor with peanut-shape structure based on multimode fiber(MMF) is proposed and experimentally demonstrated. The sensing structure and magnetic fluid(MF) are both encapsulated in capillary, and the effective refractive index of MF is affected by surrounding magnetic field strength. The measurement of magnetic field is realized by observing the wavelength drift of interference peak. The transmission spectrum generated by Mach-Zehnder interferometer(MZI) includes core-core mode interference and core-cladding mode interference. Experimental results demonstrate that the core-cladding mode interference is sensitive to magnetic field, and the magnetic field sensitivity is 0.047 8 nm/mT. In addition, two kinds of interference dips are sensitive to temperature, and the sensitivities are 0.060 0 nm/°C and 0.052 6 nm/°C, respectively. So the simultaneous measurement of magnetic field strength and temperature can be achieved based on sensitivity matrix.

A tension insensitive PbS fiber temperature sensor based on Sagnac interferometer

In this paper, a tension insensitive Pb S fiber temperature sensor based on Sagnac interferometer is proposed and demonstrated. The sensing mechanism of tension and temperature is analyzed. The relationships between the interference spectrum, temperature and tension are analyzed, respectively. The experimental temperature range is 36—70 °C. The experimental results show that the interference spectrum is red shifted, and its sensitivity is 53.89 pm/°C. In tension experiment, the tension range is 0—1 400 με. The experimental results show that there is no wavelength shift in the interference spectrum. The sensor is immune to tension cross-sensitivity compared with other sensors. It can be used for temperature testing in aerospace, chemistry and pharmacy.