An approximately 800 bp cDNA ( Lhcb 2) encoding light_harvesting chlorophyll a/b_binding protein complex (type Ⅱ) was cloned from the seedling of pea ( Pisum sativum L.) with RT_PCR method. Southern blotting usi...An approximately 800 bp cDNA ( Lhcb 2) encoding light_harvesting chlorophyll a/b_binding protein complex (type Ⅱ) was cloned from the seedling of pea ( Pisum sativum L.) with RT_PCR method. Southern blotting using special probe demonstrated that there existed one copy of Lhcb 2 in pea genome. RT_PCR and Northern blotting revealed the expression of Lhcb 2 which was regulated by light in a time_dependent expression manner. The Lhcb 2 gene didn't express untill 2 h after irradiated with white light. Low temperature (4 ℃) also affected the Lhcb 2 gene by decreasing half of its expression under 25 ℃.展开更多
The effects of temperature, light, pH value and storage time on germina- tion of Adelostemma gracillimum seeds were studied through germination experi- ment. The results showed that A. gracillimum seeds had no propert...The effects of temperature, light, pH value and storage time on germina- tion of Adelostemma gracillimum seeds were studied through germination experi- ment. The results showed that A. gracillimum seeds had no property of post-matu- ration dormancy; the optimum germination temperature was 25 ~C; no significant difference was found in germination rate of A. gracillimum seeds between light and dark conditions; A. gracillimum seeds showed a wide adaptability to pH, Le., they could germinate in the pH range of 2-10, and showed a higher germination rate in the pH range of 6-8. The optimum storage time for A. gracillimum seeds was 12 months.展开更多
In this study, the influences of various environmental factors on the degradation of deoxynivalenol （DON） in wheat grains were investigated using a triple quadrupole LC-MS/MS system. After being treated under differ...In this study, the influences of various environmental factors on the degradation of deoxynivalenol （DON） in wheat grains were investigated using a triple quadrupole LC-MS/MS system. After being treated under different conditions for 150 d, DON toxin in wheat grains exhibited the degradation rate of 78.85%-87.00% and degradation half-life of 54.57-71.44 d. According to the accelerating effects of different conditions on DON degradation, natural light 〉 strong light （10 000 lx） 〉 darkness, 90% humidity 〉50% humidity, but high temperature could not accelerate the degradation of DON toxin.展开更多
文摘An approximately 800 bp cDNA ( Lhcb 2) encoding light_harvesting chlorophyll a/b_binding protein complex (type Ⅱ) was cloned from the seedling of pea ( Pisum sativum L.) with RT_PCR method. Southern blotting using special probe demonstrated that there existed one copy of Lhcb 2 in pea genome. RT_PCR and Northern blotting revealed the expression of Lhcb 2 which was regulated by light in a time_dependent expression manner. The Lhcb 2 gene didn't express untill 2 h after irradiated with white light. Low temperature (4 ℃) also affected the Lhcb 2 gene by decreasing half of its expression under 25 ℃.
文摘The effects of temperature, light, pH value and storage time on germina- tion of Adelostemma gracillimum seeds were studied through germination experi- ment. The results showed that A. gracillimum seeds had no property of post-matu- ration dormancy; the optimum germination temperature was 25 ~C; no significant difference was found in germination rate of A. gracillimum seeds between light and dark conditions; A. gracillimum seeds showed a wide adaptability to pH, Le., they could germinate in the pH range of 2-10, and showed a higher germination rate in the pH range of 6-8. The optimum storage time for A. gracillimum seeds was 12 months.
基金Supported by the Youth Innovation Fund of President of Anhui Academy of Agricultural Sciences(13B1144)Key Science and Technology Project of Anhui Province(1301032138)+1 种基金Special Basic Research Fund(2013FY113400)Scientific and Technological Innovation Team Project of Anhui Academy of Agricultural Sciences(14C1105)~~
文摘In this study, the influences of various environmental factors on the degradation of deoxynivalenol （DON） in wheat grains were investigated using a triple quadrupole LC-MS/MS system. After being treated under different conditions for 150 d, DON toxin in wheat grains exhibited the degradation rate of 78.85%-87.00% and degradation half-life of 54.57-71.44 d. According to the accelerating effects of different conditions on DON degradation, natural light 〉 strong light （10 000 lx） 〉 darkness, 90% humidity 〉50% humidity, but high temperature could not accelerate the degradation of DON toxin.
