小麦RAPD分析中温度循环参数的优化

为了解决小麦材料 RAPD分析中的重复性差、多态性低等问题 ,对反应中的温度循环参数进行了优化研究。通过比较不同变性时间、复性温度和升温速度等的扩增效果 ,设计出了适于小麦 RAPD分析的 PCR程序 :94℃预变性 5 m in;开始的 5个循环为 94℃ 1min,38℃ 1m in,0 .3℃ / s升温 ,72℃ 2 min;后 40个循环为 94℃ 10 s,38℃ 1min,0 .3℃ / s升温 ,72℃ 2 min;最后 72℃ 5 m in。在后 40个循环中用 1～ 2 s的变性时间 ,则能够有目的地扩增 6 0 0 bp左右及以下片段。利用该程序 ,每条引物检测的位点数平均达 17.6个左右 ,高于一般报道的 1～ 10个。该程序在小麦遗传多样性研究。

Effect of wall temperature and random distribution of micro organic dust particles on their combustion parameters

The effect of wall temperature on the characteristics of random combustion of micro organic particles with recirculation was investigated. The effect of recirculating in micro-combustors is noticeable, hence it is necessary to present a model to describe the combustion process in these technologies. Recirculation phenomenon is evaluated by entering the exhausted heat from the post flam zone into the preheat zone. In this work, for modeling of random situation at the flame front, the source term in the equation of energy was modeled considering random situation for volatizing of particles in preheat zone. The comparison of obtained results from the proposed model by experimental data regards that the random model has a better agreement with experimental data than non-random model. Also, according to the results obtained by this model, wall temperature affects the amount of heat recirculation directly and higher values of wall temperature will lead to higher amounts of burning velocity and flame temperature.