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大鼠脊髓钝挫伤后单核细胞趋化蛋白-1与趋化因子受体2的表达变化 被引量:3
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作者 李盈洁 郑莉 +8 位作者 张华阳 黄守娟 易学良 胡析 牛英杰 任艳茹 杜子君 张晓 梁楠 《解剖学杂志》 CAS CSCD 2018年第6期677-681,共5页
目的:探讨单核细胞趋化蛋白-1(MCP-1)和趋化因子受体2(CCR2)在脊髓钝挫伤(SCC)大鼠的表达及其对运动功能和感觉功能的影响。方法:SD大鼠随机分为损伤(SCC组)术后12h、3d、7d、14d组及假手术组共5组。Allen打击法建立SCC模型,进行BBB评分... 目的:探讨单核细胞趋化蛋白-1(MCP-1)和趋化因子受体2(CCR2)在脊髓钝挫伤(SCC)大鼠的表达及其对运动功能和感觉功能的影响。方法:SD大鼠随机分为损伤(SCC组)术后12h、3d、7d、14d组及假手术组共5组。Allen打击法建立SCC模型,进行BBB评分,光热尾痛法测定大鼠温痛觉潜伏期,RT-PCR检测CCR2和MCP-1基因相对表达量,免疫组织化学观察MCP-1和CCR2在脊髓前角和后角的表达。结果:SCC后BBB评分降低,7d与14dBBB评分明显增高。SCC后3、7d温痛觉潜伏期较假手术组缩短。SCC后CCR2mRNA在12h和3d时表达量增高;随后在7、14dCCR2mRNA降低。MCP-1mRNA的表达量在12h、3d、7d增高,14dMCP-1mRNA下降。MCP-1和CCR2在脊髓前角主要表达在神经元中,在脊髓后角主要表达于神经胶质细胞和神经元。结论:MCP-1和CCR2可能作为重要的因子参与脊髓损伤早期的继发性损伤,导致大鼠运动功能和感觉功能障碍。 展开更多
关键词 脊髓钝挫伤 单核细胞趋化蛋白-1 受体趋化因子受体2 运动功能 温痛觉 大鼠
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miR-449a对大鼠脊髓损伤后运动功能的影响
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作者 朱美圆 牛瑞泽 +2 位作者 梁长雄 石兰岚 吴春妍 《中文科技期刊数据库(全文版)医药卫生》 2022年第2期22-24,共3页
探讨miR-449a对大鼠脊髓损伤后运动功能的影响情况。方法:SD大鼠制备SCI模型后,用大鼠甩尾实验来检测温度痛觉阈值,观察miR-449a对大鼠脊髓损伤后的运动功能的具体影响作用。结果:甩尾实验不同时间点测试结果显示,miR-449a antagomir对... 探讨miR-449a对大鼠脊髓损伤后运动功能的影响情况。方法:SD大鼠制备SCI模型后,用大鼠甩尾实验来检测温度痛觉阈值,观察miR-449a对大鼠脊髓损伤后的运动功能的具体影响作用。结果:甩尾实验不同时间点测试结果显示,miR-449a antagomir对大鼠脊髓损伤运动功能的恢复有一定的抑制作用,各组在脊髓损伤手术第1周内均有温度痛觉阈值趋势上升的变化。结论:miR-449a对大鼠脊髓损伤后运动功能有一定的影响,其对脊髓损伤后运动功能影响的关键作用及其调控机制还有待于进一步研究。 展开更多
关键词 miR-449a 脊髓损伤 温痛觉 运动功能
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Therapeutic effect of warm needling plus acupoint sticking therapy on primary dysmenorrhea due to cold-dampness retention 被引量:1
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作者 罗开涛 占道伟 +3 位作者 范迪慧 梁海丽 金月琴 韩丑萍(译) 《Journal of Acupuncture and Tuina Science》 CSCD 2016年第3期220-224,共5页
Objective: To observe the clinical efficacy of warm needling plus acupoint sticking therapy for primary dysmenorrhea. Methods: A total of 60 eligible cases were randomly allocated into a treatment group (n=30) and... Objective: To observe the clinical efficacy of warm needling plus acupoint sticking therapy for primary dysmenorrhea. Methods: A total of 60 eligible cases were randomly allocated into a treatment group (n=30) and a control group (n=30) according to their sequence of consultation. Patients in the treatment group were treated with warm needling combined with acupoint sticking therapy, whereas patients in the control group were treated with oral Ibuprofen (Fenbid) capsules. After two menstrual cycles, the visual analogue scale (VAS) score, COX menstrual symptom scale (CMSS) and clinical efficacy were compared between the two groups. Results: After treatment, the VAS and CMSS scores were significantly reduced in both groups, indicating that both treatment protocols can remarkably alleviate pain. There was a between-group statistical difference in comparing the CMSS score (P〈0.05), but no between-group statistical difference in the VAS score (P〉0.05). However, the follow-up visit showed between-group statistical differences in comparing VOA and CMSS scores (both P〈0.01). The clinical effect, relapse rate and long-term efficacy in the treatment group were better than those in the control group. Conclusion: Warm needling plus acupoint sticking therapy is a simple but effective therapy for primary dysmenorrhea. In addition, it causes fewer cases with relapse. 展开更多
关键词 Warm Needling Therapy Acupoint Sticking Therapy DYSMENORRHEA Visual Analog Scale Pain Measurement
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Clinical efficacy comparison of moxibustion with different doses for knee osteoarthritis 被引量:2
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作者 Wu Yi-wen Dai Ming +1 位作者 Chen Bi-song Chen Jing 《Journal of Acupuncture and Tuina Science》 CSCD 2020年第5期390-395,共6页
Objective:To compare the efficacy of moxibustion with different doses for knee osteoarthritis(KOA),and explore the correlation between moxibustion dose and clinical efficacy.Methods:Sixty-eight patients with KOA who m... Objective:To compare the efficacy of moxibustion with different doses for knee osteoarthritis(KOA),and explore the correlation between moxibustion dose and clinical efficacy.Methods:Sixty-eight patients with KOA who met the inclusion criteria were randomly divided into a 20-minute moxibustion group and a 40-minute moxibustion group by the random number table method,with 34 cases in each group.Dubi(ST 35),Neixiyan(EX-LE 4)and Heding(EX-LE 2)were used for moxibustion in the two groups.Each treatment lasted 20 min or 40 min for each point in the 20-minute moxibustion group and 40-minute moxibustion group,separately;the treatment was given 3 times a week and lasted for 4 weeks.The visual analog scale(VAS),Western Ontario and McMaster University osteoarthritis index(WOMAC)and traditional Chinese medicine(TCM)symptom scores were evaluated before and after treatment to compare the efficacy between different moxibustion doses for KOA.Results:After treatment,the total effective rate was 87.5%in the 40-minute moxibustion group,versus 70.0%in the 20-minute moxibustion group,and the difference in the total effective rate between the two groups was statistically significant(P<0.05).After treatment,the VAS scores,the total WOMAC scores and the component scores of pain,stiffness and dysfunction,and the TCM symptom scores in both groups all changed significantly when compared with those before treatment(all P<0.05).After treatment,the between-group differences in the VAS score,the total WOMAC score and the component scores of pain and dysfunction,and the TCM symptom score were statistically significant(all P<0.05),while the difference in the stiffness score in WOMAC showed no statistical significance(P>0.05).Conclusion:Either 20-minute moxibustion or 40-minute moxibustion can relieve pain,improve stiffness,dysfunction,and TCM symptoms for KOA;and 40-minute moxibustion is better in relieving pain,improving dysfunction and TCM symptoms. 展开更多
关键词 Moxibustion Therapy Thermal Box Moxibustion Moxibustion Quantity MOXA Pain Measurement Visual Analog Scale Osteoarthritis Knee
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Warm needling moxibustion plus functional exercises for knee osteoarthritis 被引量:7
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作者 Zhang Kai-yong Yang Yang +3 位作者 Shou Yin Xu Si-wei Jiang Hui-ru Zhang Bi-meng 《Journal of Acupuncture and Tuina Science》 CSCD 2016年第6期412-415,共4页
Objective: To observe the clinical efficacy of warm needling moxibustion plus functional exercises in treating knee osteoarthritis(KOA). Methods: Totally 108 eligible KOA patients were intervened by warm needling ... Objective: To observe the clinical efficacy of warm needling moxibustion plus functional exercises in treating knee osteoarthritis(KOA). Methods: Totally 108 eligible KOA patients were intervened by warm needling moxibustion plus functional exercises. The warm needling moxibustion was given 3 times a week, successively for 4 weeks. The visual analogue scale(VAS) was adopted to evaluate the pain intensity of the knee joint. After 4-week treatment, the change of VAS score, range of motion(ROM) of knee, and Lysholm score were observed, and the clinical efficacy was also estimated. Results: After treatment, the VAS score dropped obviously, and the ROM and Lysholm score increased markedly; the total effective rate was 83.3%. Conclusion: Warm needling moxibustion plus functional exercises is effective in treating KOA, as it can relieve the knee joint pain and improve the joint motion. 展开更多
关键词 Acupuncture Therapy Moxibustion Therapy Acupuncture-moxibustion Therapy Warm Needling Therapy Visual Analog Scale Pain Measurement OSTEOARTHRITIS KNEE
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