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温育温度对ELISA一步法检测HBsAg的影响 被引量:1
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作者 周晖 黄艳冰 《细胞与分子免疫学杂志》 CAS CSCD 2000年第6期547-547,共1页
关键词 质量控制 温育温度 HBSAG ELISA
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用于基因分析的反应系统
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作者 沈希 《浙江工业大学学报》 CAS 1997年第3期206-210,共5页
根据控制目标和硬件特点,对温育温度的控制策略进行了研究。在智能控制和PID相结合的基础上,采用了具有独创的概率控制技术,并提出了将PCR和InsituPCR两种系统综合在一起的独到设计。
关键词 基因分析 聚合酶链反应 原位杂交 温育温度
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TEMPERATURE DEPENDENT DEVELOPMENT OF ASIAN CORN BORER Ostrinia furnacalis 被引量:7
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作者 李正跃 《Zoological Research》 CAS CSCD 1998年第5期389-396,共8页
Temperature dependent development in the Asian corn borer, Ostrinia furnacalis (Guenee) was determined at nine constant temperatures between 10℃ and 34℃. Except for 10℃ development of all life stages occurred a... Temperature dependent development in the Asian corn borer, Ostrinia furnacalis (Guenee) was determined at nine constant temperatures between 10℃ and 34℃. Except for 10℃ development of all life stages occurred at the temperatures tested, however, mortality was significantly great at the extreme temperatures(12℃ and 34℃). Egg, larvae and pupae duration accounted for 17%, 57% and 25% of total one of immature stage, respectively Lower developmental thresholds estimated to be 10 38, 10 06 and 11 07℃ for eggs, larvae and pupae, respectively. Upper limited thresholds were 28 00, 31 00 and 31 00℃ for eggs, larvae and pupae, respectively. The heat unit requirements for egg stage were 79 15 degree days, for larval stage were 339 73 degree days, and for pupal stage were 128 82 degree days, respectively. Overall, heat unit requirements for development from egg to adult were 539 91 degree days between lower developmental threshold 10 35℃ and upper limited threshold 32℃. 展开更多
关键词 Asian corn borer Temperature development Thresholds Degree days
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Effect of High Temperature on Outcrossing Characteristics at the Fertility Sensitive Period of Photo-thermo Sensitive Genic Male Sterile (PTGMS) Rice Lines
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作者 王明 刘烨 +6 位作者 张海清 刘爱民 肖层林 唐荣 杨永标 蒋凌云 李雪雪 《Agricultural Science & Technology》 CAS 2017年第11期2019-2025,共7页
Four sterile lines (Peiai64S, Y58S, Guangzhan 63-2S and H638S) and the restoring line R527 were used as materials. Five temperature gradients (24, 27, 30, 33 and 36 ℃ in artificial climate chamber) and the natura... Four sterile lines (Peiai64S, Y58S, Guangzhan 63-2S and H638S) and the restoring line R527 were used as materials. Five temperature gradients (24, 27, 30, 33 and 36 ℃ in artificial climate chamber) and the natural temperature (as control) were treated to the four sterile lines for 6 d in the fertility sensitive period of heading to flowering stage, respectively, to study the effects of temperature on physiological biochemical characteristics of young panicles and outcrossing characteristics. The results showed that the percentages of exerted stigma of Peiai 64S and Y58S were the highest at 27 ℃, which were 86.81% and 86.06%, respectively, while the percentages of exerted stigma of Guangzhan 63-2S and H638S were the highest at 24 ℃, which were 76.24% and 81.76%, respectively; the stigma viability of Peiai 64S, Y58S, Guangzhan 63-2S and H638S were the best at 24 ℃, which were 1.96, 2.12, 1.74 and 1.94, respectively; the outcrossing rates of Peiai 64S, Guangzhan 63-2S and H638S were the highest at 24 ℃, which were 58.87%, 54.22% and 50.50%, respectively, while the outcrossing rate of Y58S was the highest at 27 ℃, which was 58.96%; and the contents of peroxidase (POD) of the four sterile lines increased significantly at 33 ℃ compared with the control, while the contents of malondialdehyde (MDA) and proline increased significantly at 36 ℃ compared with the control. There were differences in temperature sensitivity between the male sterile lines, and the 24 ℃ treatment during the sensitive period was the best for the fertility sensitive period of Peiai 64S, while 27 ℃ was the best temperature for Y58S, Gangzhan 63-2S and H638S. 展开更多
关键词 RICE Photo-thermo sensitive male sterile line Fertility sensitive period Temperature Outcrossing characteristics Physiological and biochemical characteristics
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