Electrocardiograms (ECG) of Eremias multiocellata were studied at 5-35℃ in body temperature. Electrocardiogram wave intervals (R-R,P-R,QRS,T-P,and R-T) shortened while heart rate increased with the increasing of bod...Electrocardiograms (ECG) of Eremias multiocellata were studied at 5-35℃ in body temperature. Electrocardiogram wave intervals (R-R,P-R,QRS,T-P,and R-T) shortened while heart rate increased with the increasing of body temperature. The average heart rate was 14.6/min at 5℃,whereas it was 201/min at 35℃. The duration of wave intervals of ECG and the heart rate were related significantly to the body temperature (P<0.001). Among the components of a cardiac cycle the cardiac rest period (TP intervals) and the atria-ventricular conduction time (PR interval) were affected mostly by body temperature. In the other hand the ventricular depolarization and repolarization (QRS and R-T intervals) were relatively less affected by the body temperature. The increasing of heart rate with body temperature was mainly caused by the shortening of ECG wave intervals,and the T-P interval (the cardiac rest period) was shortened more noticeably than other intervals.展开更多
The decadal variability of the North Atlantic thermohaline circulation(THC) is investigated within a three-dimensional ocean circulation model using the conditional nonlinear optimal perturbation method. The results s...The decadal variability of the North Atlantic thermohaline circulation(THC) is investigated within a three-dimensional ocean circulation model using the conditional nonlinear optimal perturbation method. The results show that the optimal initial perturbations of temperature and salinity exciting the strongest decadal THC variations have similar structures: the perturbations are mainly in the northwestern basin at a depth ranging from 1500 to 3000 m. These temperature and salinity perturbations act as the optimal precursors for future modifications of the THC, highlighting the importance of observations in the northwestern basin to monitor the variations of temperature and salinity at depth. The decadal THC variation in the nonlinear model initialized by the optimal salinity perturbations is much stronger than that caused by the optimal temperature perturbations, indicating that salinity variations might play a relatively important role in exciting the decadal THC variability. Moreover, the decadal THC variations in the tangent linear and nonlinear models show remarkably different characteristics, suggesting the importance of nonlinear processes in the decadal variability of the THC.展开更多
Objective: There is a remarkable lack of scientific evidence to support the option to use alpha-stat or pH-stat management, as to which is more beneficial to brain protection during deep hypothermic CPB. This study ex...Objective: There is a remarkable lack of scientific evidence to support the option to use alpha-stat or pH-stat management, as to which is more beneficial to brain protection during deep hypothermic CPB. This study examined cortical blood flow (CBF), cerebral oxygenation, and brain oxygen consumption in relation to deep hypothermic CPB with alpha-stat or pH-stat management. Methods: Twenty-two pigs were cooled with alpha-stat or pH-stat during CPB to 15℃ esophageal temperature. CBF and cerebral oxygenation were measured continuously with a laser flowmeter and near-infrared spec-troscopy, respectively. Brain oxygen consumption was measured with standard laboratory techniques. Results: During CPB cooling, CBF was significantly decreased, about 52,2%±6.3% (P<0.01 vs 92.6%±6.5% of pH-stat) at 15℃ in alpha-stat, whereas there were no significant changes in CBF in pH-stat. While cooling down, brain oxygen extraction (OER) progressively decreased, about 9.5%±0.9% and 10.9%±1.5% at 15℃ in alpha-stat and pH-stat, respectively. At 31℃ the decreased value in pH-stat was lower than in alpha-stat (29.9%±2.7% vs 22.5%±1.9%; P<0.05). The ratio of CBF/OER were 2.0±0.3 in alpha-stat and pH-stat, respectively; it was kept in constant level in alpha-stat, and significantly increased by 19 ℃ to 15℃ in pH-stat (4.9±0.9 vs 2.3±0.4; P<0.01). In mild hypothermia, cerebral oxyhemoglobin and oxygen saturation in alpha-stat were greater than that in pH-stat (102.5%±1.4% vs 99.1%±0.7%; P<0.05). In deep hypothermia, brain oxygen saturation in pH-stat was greater than that in alpha-stat (99.2%±1.0% vs 93.8%±1.0%; P<0.01), and deoxyhemoglobin in pH-stat decreased more greatly than that in alpha-stat (28.7%±6.8% vs 54.1%±4.7%; P<0.05). Conclusions: In mild hypothermic CPB, brain tissue oxygen saturation was greater in alpha-stat than in pH-stat. However, cerebral oxygenation and brain tissue oxygen saturation were better in pH-stat than in alpha-stat during profound hypothermia. PH-stat strategy provided much more oxygen to brain tissue before deep hypothermic circulatory arrest.展开更多
Paraplegia is a disastrous complication after operations of descending and thoracoabdominal aortic aneurysm. Re- gional hypothermia protects against spinal cord ischemia although the protective mechanism is not well k...Paraplegia is a disastrous complication after operations of descending and thoracoabdominal aortic aneurysm. Re- gional hypothermia protects against spinal cord ischemia although the protective mechanism is not well know. The objective of this study is to examine whether hypothermia protects the spinal cord by preventing apoptosis of nerve cell and also investigate a possible mechanism involved in hypothermia neuroprotection. Cell apoptosis with necrosis was evident in the spinal cord 24 h after 30 min of ischemia. Moderate hypothermia decreased the incidence of apoptotic nerve cells. Both cell apoptosis and necrosis were attenuated by hypothermia. p53 expression increased and bcl-2 expression declined after ischemia, while hypothermia mitigated these changes. This study suggests that apoptosis contributes to cell death after spinal cord ischemia, and that moderate hypothermia can prevent nerve cell apoptosis by a mechanism associated with bcl-2 and p53 genes.展开更多
In this work, sesamin and sesamolin is separated and purified by SMB （simulated moving bed） chromatography. Purity of sesamin and sesamolin can reach 99.2% and 99.9 %, and they can be recovered by 99.9% and 99.4%, r...In this work, sesamin and sesamolin is separated and purified by SMB （simulated moving bed） chromatography. Purity of sesamin and sesamolin can reach 99.2% and 99.9 %, and they can be recovered by 99.9% and 99.4%, respectively. Concentrations of the extract and raffinate was monitored and periodical change of the concentration was observed on experiment. Effects of non-linear adsorption, dead volume, and temperature on the separation are reviewed by the triangle theory to explain the periodical change of concentration. It is found that dead volume has little effect, and temperature fluctuation significantly affects thc separation by SMB. The temperature seriously affects the robust operation of SMB due to the change of Henry＇s constant, which will change the selectivity and restrain the productivity. Although the productivity in this study is only 0.0416 g/L-hr, the cost of the stationary phase used in this study is much lower than that of packed in analytical column. By using this stationary phase, the authors are still able to separate the mixture of sesamin and sesmolin. This will economically attract attention for the application of SMB to purify bioactive compounds on developing botanical drugs.展开更多
Objective To observe the effects of a protocol consisting of warm needling with moxibustion at Xiàguān（下关 ST 7） plus shallow puncture on Bell＇s palsy（BP） in pregnancy.Methods Thirteen patients with BP rec...Objective To observe the effects of a protocol consisting of warm needling with moxibustion at Xiàguān（下关 ST 7） plus shallow puncture on Bell＇s palsy（BP） in pregnancy.Methods Thirteen patients with BP received an intervention of warm needling moxibustion at ST 7 plus shallow puncture,and were evaluated using the House–Brackmann facial nerve grading system（HB） before and after intervention.Result After intervention,the HB grade improved significantly（P0.05）.Conclusion Warm needling moxibustion plus shallow puncture is a safe and effective therapy for pregnant BP patients.BP in pregnancy appears to be more frequent in the last trimester.The severity of nerve injury and the timing for the first visit to the doctor after the onset of facial paralysis are essential factors for the recovery and prognosis of pregnant with BP.展开更多
To observe the clinical effect of acupuncture combined with copper-tube moxibustion on peripheral facial paralysis. Methods： A total of 39 recurrent Bell＇s palsy patients were treated with acupuncture plus self-made...To observe the clinical effect of acupuncture combined with copper-tube moxibustion on peripheral facial paralysis. Methods： A total of 39 recurrent Bell＇s palsy patients were treated with acupuncture plus self-made copper-tube moxibustion, once a day. Ten days made up a course of treatment. The patients were treated for 4 courses of treatment. There was a 3-day interval between two courses. The therapeutic efficacies were statistically analyzed after 4 courses of treatment. Results： Of the 39 cases, 18 cases obtained recovery, 15 cases got improvement and 6 cases failed. The total effective rate was 84.6%. Conclusion： Acupuncture combined with copper-tube moxibustion is effective for recurrent peripheral facial paralysis.展开更多
Objective： To observe the clinical effect of mild moxibustion for facial paralysis. Methods： One hundred cases of facial paralysis were divided randomly into two groups： 50 cases in the treatment group were treated...Objective： To observe the clinical effect of mild moxibustion for facial paralysis. Methods： One hundred cases of facial paralysis were divided randomly into two groups： 50 cases in the treatment group were treated by mild moxibustion, and 50 cases in the control group were treated by oral administration of Cobamamide Tablets. With ten days as one course of the treatments, two courses of the treatments were given. The therapeutic effects were assessed four weeks after treatments. Results： The total effective rate was 96.0% in the treatment group and 88.0% in the control group, and there was statistical difference in the therapeutic effects between the two groups （P〈0.05）. Conclusion： The therapeutic effect of mild moxibustion for facial paralysis is better than oral administration of Cobamamide Tablets.展开更多
文摘Electrocardiograms (ECG) of Eremias multiocellata were studied at 5-35℃ in body temperature. Electrocardiogram wave intervals (R-R,P-R,QRS,T-P,and R-T) shortened while heart rate increased with the increasing of body temperature. The average heart rate was 14.6/min at 5℃,whereas it was 201/min at 35℃. The duration of wave intervals of ECG and the heart rate were related significantly to the body temperature (P<0.001). Among the components of a cardiac cycle the cardiac rest period (TP intervals) and the atria-ventricular conduction time (PR interval) were affected mostly by body temperature. In the other hand the ventricular depolarization and repolarization (QRS and R-T intervals) were relatively less affected by the body temperature. The increasing of heart rate with body temperature was mainly caused by the shortening of ECG wave intervals,and the T-P interval (the cardiac rest period) was shortened more noticeably than other intervals.
基金supported by the National Basic Research Program of China(973 Program,Grant No.2012CB417404)
文摘The decadal variability of the North Atlantic thermohaline circulation(THC) is investigated within a three-dimensional ocean circulation model using the conditional nonlinear optimal perturbation method. The results show that the optimal initial perturbations of temperature and salinity exciting the strongest decadal THC variations have similar structures: the perturbations are mainly in the northwestern basin at a depth ranging from 1500 to 3000 m. These temperature and salinity perturbations act as the optimal precursors for future modifications of the THC, highlighting the importance of observations in the northwestern basin to monitor the variations of temperature and salinity at depth. The decadal THC variation in the nonlinear model initialized by the optimal salinity perturbations is much stronger than that caused by the optimal temperature perturbations, indicating that salinity variations might play a relatively important role in exciting the decadal THC variability. Moreover, the decadal THC variations in the tangent linear and nonlinear models show remarkably different characteristics, suggesting the importance of nonlinear processes in the decadal variability of the THC.
文摘Objective: There is a remarkable lack of scientific evidence to support the option to use alpha-stat or pH-stat management, as to which is more beneficial to brain protection during deep hypothermic CPB. This study examined cortical blood flow (CBF), cerebral oxygenation, and brain oxygen consumption in relation to deep hypothermic CPB with alpha-stat or pH-stat management. Methods: Twenty-two pigs were cooled with alpha-stat or pH-stat during CPB to 15℃ esophageal temperature. CBF and cerebral oxygenation were measured continuously with a laser flowmeter and near-infrared spec-troscopy, respectively. Brain oxygen consumption was measured with standard laboratory techniques. Results: During CPB cooling, CBF was significantly decreased, about 52,2%±6.3% (P<0.01 vs 92.6%±6.5% of pH-stat) at 15℃ in alpha-stat, whereas there were no significant changes in CBF in pH-stat. While cooling down, brain oxygen extraction (OER) progressively decreased, about 9.5%±0.9% and 10.9%±1.5% at 15℃ in alpha-stat and pH-stat, respectively. At 31℃ the decreased value in pH-stat was lower than in alpha-stat (29.9%±2.7% vs 22.5%±1.9%; P<0.05). The ratio of CBF/OER were 2.0±0.3 in alpha-stat and pH-stat, respectively; it was kept in constant level in alpha-stat, and significantly increased by 19 ℃ to 15℃ in pH-stat (4.9±0.9 vs 2.3±0.4; P<0.01). In mild hypothermia, cerebral oxyhemoglobin and oxygen saturation in alpha-stat were greater than that in pH-stat (102.5%±1.4% vs 99.1%±0.7%; P<0.05). In deep hypothermia, brain oxygen saturation in pH-stat was greater than that in alpha-stat (99.2%±1.0% vs 93.8%±1.0%; P<0.01), and deoxyhemoglobin in pH-stat decreased more greatly than that in alpha-stat (28.7%±6.8% vs 54.1%±4.7%; P<0.05). Conclusions: In mild hypothermic CPB, brain tissue oxygen saturation was greater in alpha-stat than in pH-stat. However, cerebral oxygenation and brain tissue oxygen saturation were better in pH-stat than in alpha-stat during profound hypothermia. PH-stat strategy provided much more oxygen to brain tissue before deep hypothermic circulatory arrest.
基金This work was supported in part by National Natural Science Foundation of China(90208011,30300174,30470856 and 30421005)National Basic Research Program(973 Program)(2002CB713802)Shanghai Key Project of Ba-sic Science Research(04DZ14005 and 04DZ05608).
文摘Paraplegia is a disastrous complication after operations of descending and thoracoabdominal aortic aneurysm. Re- gional hypothermia protects against spinal cord ischemia although the protective mechanism is not well know. The objective of this study is to examine whether hypothermia protects the spinal cord by preventing apoptosis of nerve cell and also investigate a possible mechanism involved in hypothermia neuroprotection. Cell apoptosis with necrosis was evident in the spinal cord 24 h after 30 min of ischemia. Moderate hypothermia decreased the incidence of apoptotic nerve cells. Both cell apoptosis and necrosis were attenuated by hypothermia. p53 expression increased and bcl-2 expression declined after ischemia, while hypothermia mitigated these changes. This study suggests that apoptosis contributes to cell death after spinal cord ischemia, and that moderate hypothermia can prevent nerve cell apoptosis by a mechanism associated with bcl-2 and p53 genes.
文摘In this work, sesamin and sesamolin is separated and purified by SMB （simulated moving bed） chromatography. Purity of sesamin and sesamolin can reach 99.2% and 99.9 %, and they can be recovered by 99.9% and 99.4%, respectively. Concentrations of the extract and raffinate was monitored and periodical change of the concentration was observed on experiment. Effects of non-linear adsorption, dead volume, and temperature on the separation are reviewed by the triangle theory to explain the periodical change of concentration. It is found that dead volume has little effect, and temperature fluctuation significantly affects thc separation by SMB. The temperature seriously affects the robust operation of SMB due to the change of Henry＇s constant, which will change the selectivity and restrain the productivity. Although the productivity in this study is only 0.0416 g/L-hr, the cost of the stationary phase used in this study is much lower than that of packed in analytical column. By using this stationary phase, the authors are still able to separate the mixture of sesamin and sesmolin. This will economically attract attention for the application of SMB to purify bioactive compounds on developing botanical drugs.
基金Supported by Zhejiang Traditional Chinese Medicine Technology Program 2016ZA076
文摘Objective To observe the effects of a protocol consisting of warm needling with moxibustion at Xiàguān（下关 ST 7） plus shallow puncture on Bell＇s palsy（BP） in pregnancy.Methods Thirteen patients with BP received an intervention of warm needling moxibustion at ST 7 plus shallow puncture,and were evaluated using the House–Brackmann facial nerve grading system（HB） before and after intervention.Result After intervention,the HB grade improved significantly（P0.05）.Conclusion Warm needling moxibustion plus shallow puncture is a safe and effective therapy for pregnant BP patients.BP in pregnancy appears to be more frequent in the last trimester.The severity of nerve injury and the timing for the first visit to the doctor after the onset of facial paralysis are essential factors for the recovery and prognosis of pregnant with BP.
文摘To observe the clinical effect of acupuncture combined with copper-tube moxibustion on peripheral facial paralysis. Methods： A total of 39 recurrent Bell＇s palsy patients were treated with acupuncture plus self-made copper-tube moxibustion, once a day. Ten days made up a course of treatment. The patients were treated for 4 courses of treatment. There was a 3-day interval between two courses. The therapeutic efficacies were statistically analyzed after 4 courses of treatment. Results： Of the 39 cases, 18 cases obtained recovery, 15 cases got improvement and 6 cases failed. The total effective rate was 84.6%. Conclusion： Acupuncture combined with copper-tube moxibustion is effective for recurrent peripheral facial paralysis.
文摘Objective： To observe the clinical effect of mild moxibustion for facial paralysis. Methods： One hundred cases of facial paralysis were divided randomly into two groups： 50 cases in the treatment group were treated by mild moxibustion, and 50 cases in the control group were treated by oral administration of Cobamamide Tablets. With ten days as one course of the treatments, two courses of the treatments were given. The therapeutic effects were assessed four weeks after treatments. Results： The total effective rate was 96.0% in the treatment group and 88.0% in the control group, and there was statistical difference in the therapeutic effects between the two groups （P〈0.05）. Conclusion： The therapeutic effect of mild moxibustion for facial paralysis is better than oral administration of Cobamamide Tablets.
