The paper sets out to consider only Dan Brown＇s frequent English word games and etymologies in his last novel, asking in how far these can be translated into related and unrelated languages. Thus translation is the i...The paper sets out to consider only Dan Brown＇s frequent English word games and etymologies in his last novel, asking in how far these can be translated into related and unrelated languages. Thus translation is the issue here in so far as Brown makes certain ideas, conversations, even events, in the novel relying on English word play, which may not be translatable展开更多
The paper tries to explore how to use games in teaching English vocabulary in kindergartens. It starts with the introduction of definitions of the game and the characteristics of the game, then lists the types of the ...The paper tries to explore how to use games in teaching English vocabulary in kindergartens. It starts with the introduction of definitions of the game and the characteristics of the game, then lists the types of the game. Based on an analysis of present situation of English vocabulary teaching in kindergartens, the author points out the importance of teaching English vocabulary through games in kindergarten. Finally, the author explains how to use games to teach English vocabulary in kindergartens.展开更多
The piggyBac transposon has been long used to integrate foreign DNA into insect genomes.However,undesirable transgene expression can result from random insertions into the genome.In this study,the efficiency of chimer...The piggyBac transposon has been long used to integrate foreign DNA into insect genomes.However,undesirable transgene expression can result from random insertions into the genome.In this study,the efficiency of chimeric Gal4-piggyBac transposase in directing integration onto a DNA target plasmid was evaluated in cultured silkworm Bombyx mori Bm-12 and fruit fly Drosophila Schneider 2(S2) cells.The Gal4-piggyBac transposase has a Gal4 DNA-binding domain(DBD),and the target plasmid has upstream activating sequences(UAS) to which the Gal4 DBD can bind with high affinity.The results indicate that,in the Bm-12 and S2 cells,transpositional activity of Gal4-piggyBac transposase was increased by 4.0 and 7.5 times,respectively,compared to controls,where Gal4-UAS interaction was absent.Moreover,the Gal4-piggyBac transposase had the ability of directing piggyBac element integration to certain sites of the target plasmid,although the target-directing specificity was not as high as expected.The chimeric piggyBac transposase has the potential for use in site-directed transgenesis and gene function research in B.mori.展开更多
文摘The paper sets out to consider only Dan Brown＇s frequent English word games and etymologies in his last novel, asking in how far these can be translated into related and unrelated languages. Thus translation is the issue here in so far as Brown makes certain ideas, conversations, even events, in the novel relying on English word play, which may not be translatable
文摘The paper tries to explore how to use games in teaching English vocabulary in kindergartens. It starts with the introduction of definitions of the game and the characteristics of the game, then lists the types of the game. Based on an analysis of present situation of English vocabulary teaching in kindergartens, the author points out the importance of teaching English vocabulary through games in kindergarten. Finally, the author explains how to use games to teach English vocabulary in kindergartens.
基金supported by the National Basic Research Program (973) of China (No. 2009CB119200)the Natural Science Foundation of Zhejiang Province (No. Y304122),China
文摘The piggyBac transposon has been long used to integrate foreign DNA into insect genomes.However,undesirable transgene expression can result from random insertions into the genome.In this study,the efficiency of chimeric Gal4-piggyBac transposase in directing integration onto a DNA target plasmid was evaluated in cultured silkworm Bombyx mori Bm-12 and fruit fly Drosophila Schneider 2(S2) cells.The Gal4-piggyBac transposase has a Gal4 DNA-binding domain(DBD),and the target plasmid has upstream activating sequences(UAS) to which the Gal4 DBD can bind with high affinity.The results indicate that,in the Bm-12 and S2 cells,transpositional activity of Gal4-piggyBac transposase was increased by 4.0 and 7.5 times,respectively,compared to controls,where Gal4-UAS interaction was absent.Moreover,the Gal4-piggyBac transposase had the ability of directing piggyBac element integration to certain sites of the target plasmid,although the target-directing specificity was not as high as expected.The chimeric piggyBac transposase has the potential for use in site-directed transgenesis and gene function research in B.mori.
