The wettability and interfacial reactions of four kinds of PdNi-based brazing fillers on C-C composite were studied with the sessile drop method.The results showed that the wettability of these brazing fillers was imp...The wettability and interfacial reactions of four kinds of PdNi-based brazing fillers on C-C composite were studied with the sessile drop method.The results showed that the wettability of these brazing fillers was improved with the increase of Cr content. Cr distributed at the interface of brazing filler/C-C composite and the formation of Cr23C6 phase was speculated.In the interface between Ni-33Cr-24Pd-4Si brazing filler and C-C composite,element Cr reacted with C-C to form Cr-C reaction layer.Pd together with Si participated in the interfacial reactions and formed Pd2Si and Pd3Si phases.Furthermore,in this reaction zone,the residual brazing alloy became Ni-rich and Pd-depleted.展开更多
This study differentiated pseudocondyloma of vulva from condyloma acuminata using dot blot hybridization and polymerase chain reaction (PCR). A total of 27 cases of pseudocondyloma of vulva and 65 cases of condyloma a...This study differentiated pseudocondyloma of vulva from condyloma acuminata using dot blot hybridization and polymerase chain reaction (PCR). A total of 27 cases of pseudocondyloma of vulva and 65 cases of condyloma acuminata were selected for the study. The genital lesions were examined clinically and were biopsied. Each biopsy was subjected to histological examination and HPV DNA analysis by dot blot hybridization and PCR. Dot blot analysis detected HPV DNA in 19(82. 6%) out of 23 cases of condyloma acuminata and 2(25% ) out of 8 cases pseudocondyloma of vulvae (P<0. 05). PCR detected HPV DNA in 51 (92. 7%) out of 55 cases of condyloma acuminata , compared with none in 23 cases of pseudocondyloma (P<0.001 ). HPV DNA was present in the majority of condyloma acuminata specimens. HPV 6 and 11 were the predominant types. Peudocondyloma is probably not associated with HPV. PCR was the most sensitive and useful technique for HPV DNA detection.展开更多
This study was undertaken to investigate the regulatory effect of Resveratrol (Res) on the proliferation and apop- tosis of synoviocytes of patients with rheumatoid arthritis (RA), as the proliferation of synoviocytes...This study was undertaken to investigate the regulatory effect of Resveratrol (Res) on the proliferation and apop- tosis of synoviocytes of patients with rheumatoid arthritis (RA), as the proliferation of synoviocytes of patients was deter- mined by MTT chromatometry and the apoptosis of these cells was assayed with TUNEL flow cytometry. It was found in this experiment that the degree of cell proliferation of the Res-treated group with dosages of 50-400 μM was significantly reduced in comparison with that of the control group, but percentage of the apoptotic cells demonstrated with TUNEL labeling was el- evated under treatment with Res at the same dosages in a concentration-dependent manner. The difference between the Res- treated group and the control group was quite significant ( P < 0.01). It is concluded that Res shows a potent anti-prolifera- tive effect on synoviocytes of patients with RA with induction of cell apoptosis, and it is likely a valuable candidate for the chemotherapy and management of patients with RA.展开更多
Objective To investigate the regulatory mechanisms of acetylated p53 in the expression of microtubule-associated protein-2(MAP2) in neuronal differentiation of P19 cells induced by all-trans retinoic acid(RA).Methods ...Objective To investigate the regulatory mechanisms of acetylated p53 in the expression of microtubule-associated protein-2(MAP2) in neuronal differentiation of P19 cells induced by all-trans retinoic acid(RA).Methods Neuronal differentiation of P19 cells was initiated with 4-day RA treatment.Immunofluorescence,real-time reverse transcription-polymerase chain reaction(RT-PCR) assay,and map2 promoter driven luciferase assay were performed to detect the expression and relative promoter activity of MAP2 in those RA-treated cells.Real-time PCR-based chromatin immunoprecipitation assay(ChIP) was carried out to reveal the specific recruitment of acetylated p53 onto its binding sites on map2 promoter.Results The expression of MAP2 was markedly increased in RA-induced P19 cells.The map2 mRNA increased 34-fold after 4 days of RA treatment and 730-fold 2 days after the treatment,compared with the cells without RA treatment(control).p53 was recruited to the promoter of map2 gene in acetylated form and thereby enhanced its promoter activity.p300/CBP associated factor(PCAF) was found induced in RA-treated cells and enriched in the nucleus,which might contribute to the acetylation of p53 in the regulation of map2 gene.Conclusions Acetylated p53 may participate in regulating the expression of map2 in RA-induced differentiation of P19 cells.PCAF is possibly involved in this process by mediating the acetylation of p53.展开更多
基金Projects(59905022,50475160)supported by the National Natural Science Foundation of China
文摘The wettability and interfacial reactions of four kinds of PdNi-based brazing fillers on C-C composite were studied with the sessile drop method.The results showed that the wettability of these brazing fillers was improved with the increase of Cr content. Cr distributed at the interface of brazing filler/C-C composite and the formation of Cr23C6 phase was speculated.In the interface between Ni-33Cr-24Pd-4Si brazing filler and C-C composite,element Cr reacted with C-C to form Cr-C reaction layer.Pd together with Si participated in the interfacial reactions and formed Pd2Si and Pd3Si phases.Furthermore,in this reaction zone,the residual brazing alloy became Ni-rich and Pd-depleted.
文摘This study differentiated pseudocondyloma of vulva from condyloma acuminata using dot blot hybridization and polymerase chain reaction (PCR). A total of 27 cases of pseudocondyloma of vulva and 65 cases of condyloma acuminata were selected for the study. The genital lesions were examined clinically and were biopsied. Each biopsy was subjected to histological examination and HPV DNA analysis by dot blot hybridization and PCR. Dot blot analysis detected HPV DNA in 19(82. 6%) out of 23 cases of condyloma acuminata and 2(25% ) out of 8 cases pseudocondyloma of vulvae (P<0. 05). PCR detected HPV DNA in 51 (92. 7%) out of 55 cases of condyloma acuminata , compared with none in 23 cases of pseudocondyloma (P<0.001 ). HPV DNA was present in the majority of condyloma acuminata specimens. HPV 6 and 11 were the predominant types. Peudocondyloma is probably not associated with HPV. PCR was the most sensitive and useful technique for HPV DNA detection.
文摘This study was undertaken to investigate the regulatory effect of Resveratrol (Res) on the proliferation and apop- tosis of synoviocytes of patients with rheumatoid arthritis (RA), as the proliferation of synoviocytes of patients was deter- mined by MTT chromatometry and the apoptosis of these cells was assayed with TUNEL flow cytometry. It was found in this experiment that the degree of cell proliferation of the Res-treated group with dosages of 50-400 μM was significantly reduced in comparison with that of the control group, but percentage of the apoptotic cells demonstrated with TUNEL labeling was el- evated under treatment with Res at the same dosages in a concentration-dependent manner. The difference between the Res- treated group and the control group was quite significant ( P < 0.01). It is concluded that Res shows a potent anti-prolifera- tive effect on synoviocytes of patients with RA with induction of cell apoptosis, and it is likely a valuable candidate for the chemotherapy and management of patients with RA.
基金Supported by National Natural Science Foundation of China (30871382,30721063)National Basic Research Program of China (973 Program) (2005CB522405)Special Funds of State Key Laboratories (2060204)
文摘Objective To investigate the regulatory mechanisms of acetylated p53 in the expression of microtubule-associated protein-2(MAP2) in neuronal differentiation of P19 cells induced by all-trans retinoic acid(RA).Methods Neuronal differentiation of P19 cells was initiated with 4-day RA treatment.Immunofluorescence,real-time reverse transcription-polymerase chain reaction(RT-PCR) assay,and map2 promoter driven luciferase assay were performed to detect the expression and relative promoter activity of MAP2 in those RA-treated cells.Real-time PCR-based chromatin immunoprecipitation assay(ChIP) was carried out to reveal the specific recruitment of acetylated p53 onto its binding sites on map2 promoter.Results The expression of MAP2 was markedly increased in RA-induced P19 cells.The map2 mRNA increased 34-fold after 4 days of RA treatment and 730-fold 2 days after the treatment,compared with the cells without RA treatment(control).p53 was recruited to the promoter of map2 gene in acetylated form and thereby enhanced its promoter activity.p300/CBP associated factor(PCAF) was found induced in RA-treated cells and enriched in the nucleus,which might contribute to the acetylation of p53 in the regulation of map2 gene.Conclusions Acetylated p53 may participate in regulating the expression of map2 in RA-induced differentiation of P19 cells.PCAF is possibly involved in this process by mediating the acetylation of p53.
