To solve the problems of long seedling culture time and poor quality of tobacco seedlings bred by floating culture technique of seedlings in high-altitude tobacco areas, a comparative trial between seedling nursing on...To solve the problems of long seedling culture time and poor quality of tobacco seedlings bred by floating culture technique of seedlings in high-altitude tobacco areas, a comparative trial between seedling nursing on moist trays ＋ fermentation heating using farmyard manure and conventional floating culture of seedlings in Fenggang County was carried out in 2013. The results showed that seedling nursing on moist trays ＋ fermentation heating using farmyard manure can improve 4-5 ℃ of pool water temperature, shorten 8 d of seedling age, increase 4.45 cm of seedling height, thicken 0.46 cm of stem girth and make lateral root system developed. The method can shorten the time of floating breeding of flue-cured tobacco,improve the quality of tobacco seedlings, thus it was suitable to popularize in highaltitude tobacco areas of Fenggang County.展开更多
To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immu-nogenicity in mice. Methods The major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into ...To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immu-nogenicity in mice. Methods The major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into an eukaryotic ex-pression plasmid (pcDNA3.1). The recombinant plasmid was transfected into COS-7 cells. Western blot were performed to detect whether L1 protein can be expressed in eukaryotic cells. Eighteen female BALB/c mice were tested for immunoge-nicity study. Results The recombinant plasmid (pcDNA3.1-HPV6bL1) was verified as HPV6b L1 gene by sequencing. Western blot showed specific strip. Anti-L1 protein antibodies could be detected in the mice’s sera inoculated with pcDNA3.1-HPV6bL1. Similarly, IL-4, IL-2, and IFN-γ were increased in the same mice. Conclusion HPV6b L1 recombinant plasmid was constructed successfully which had immunogenicity for BALB/c mice. It provided experimental evidence for the research of DNA vaccine of condyloma acuminata..-展开更多
基金Supported by the Major Project of Zunyi Tobacco Company-"The Demonstration Zone of Characteristic Flue-Cured Tobacco Varieties in Fenggang County"(Contract No.2014-20)~~
文摘To solve the problems of long seedling culture time and poor quality of tobacco seedlings bred by floating culture technique of seedlings in high-altitude tobacco areas, a comparative trial between seedling nursing on moist trays ＋ fermentation heating using farmyard manure and conventional floating culture of seedlings in Fenggang County was carried out in 2013. The results showed that seedling nursing on moist trays ＋ fermentation heating using farmyard manure can improve 4-5 ℃ of pool water temperature, shorten 8 d of seedling age, increase 4.45 cm of seedling height, thicken 0.46 cm of stem girth and make lateral root system developed. The method can shorten the time of floating breeding of flue-cured tobacco,improve the quality of tobacco seedlings, thus it was suitable to popularize in highaltitude tobacco areas of Fenggang County.
文摘To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immu-nogenicity in mice. Methods The major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into an eukaryotic ex-pression plasmid (pcDNA3.1). The recombinant plasmid was transfected into COS-7 cells. Western blot were performed to detect whether L1 protein can be expressed in eukaryotic cells. Eighteen female BALB/c mice were tested for immunoge-nicity study. Results The recombinant plasmid (pcDNA3.1-HPV6bL1) was verified as HPV6b L1 gene by sequencing. Western blot showed specific strip. Anti-L1 protein antibodies could be detected in the mice’s sera inoculated with pcDNA3.1-HPV6bL1. Similarly, IL-4, IL-2, and IFN-γ were increased in the same mice. Conclusion HPV6b L1 recombinant plasmid was constructed successfully which had immunogenicity for BALB/c mice. It provided experimental evidence for the research of DNA vaccine of condyloma acuminata..-
