目的:转录因子NF-κB的诱导,调控着免疫和炎症反应中众多基因的表达。溃疡性结肠炎(ulcerative colitis,UC)存在上皮细胞、淋巴细胞、巨噬细胞的异常激活及细胞因子的表达失调。我们检测了UC组织中NF-κBp65,及两种启动子含NF-κB结合...目的:转录因子NF-κB的诱导,调控着免疫和炎症反应中众多基因的表达。溃疡性结肠炎(ulcerative colitis,UC)存在上皮细胞、淋巴细胞、巨噬细胞的异常激活及细胞因子的表达失调。我们检测了UC组织中NF-κBp65,及两种启动子含NF-κB结合位点的蛋白COX-2和iNOS的表达和分布,探讨他们在UC发病机制中的作用,及三者之间的关系。 方法:用免疫组化SP法检测39例活动期溃疡性结肠炎内镜活检标本及30例正常对照石蜡包埋组织中NF-κBp65,COX-2和iNOS的表达情况。 结果:UC组p65,COX-2和iNOS均为阳性表达,主要分布于上皮细胞,固有层炎性细胞及血管内皮细胞也有程度不同的表达。对照组均为阴性或弱阳性表达。三者在UC组的表达与对照组相比,差异均有非常显著性(P<0.01)。p65的表达与内镜及病理分级有关,内镜Ⅱ级与Ⅰ级比较(5.8±2.6 vs 3.6±1.9),差异显著(P<0.05)。病理Ⅱ,Ⅲ级与Ⅰ级比较(6.1±2.4,7.3±2.5 vs 4.0±2.3),差异显著(P<0.05,P<0.01)。iNOS的表达与病理分级有关,Ⅲ级与Ⅰ,Ⅱ级比较(7.8±2.5 vs 4.6±2.3,5.0±1.6),差异显著(P<0.01,P<0.05),COX-2的表达在病情轻重,内镜及病理分级间差异无显著性(p>0.05)。p65与COX-2,iNOS表达显著相关(r_s^1分别为0.713,0.706;P<0.01),COX-2与iNOS表达显著相关(r_s^1=0.展开更多
AIM: To observe different histomorphologic changes of ulcerative colitis (UC) rats that were treated with four regulating-intestine prescriptions (FRIP), to investigate the curative effects of FRIP and to analyze thei...AIM: To observe different histomorphologic changes of ulcerative colitis (UC) rats that were treated with four regulating-intestine prescriptions (FRIP), to investigate the curative effects of FRIP and to analyze their treatment mechanism.METHODS: The UC rat model was made by the method of 2,4-dinitro chloro benzene (DNCB) immunity and acetic acid local enema. Ninety-eight SD rats were randomly divided into seven groups, namely, the normal control group, model group, salicylazosulfapyridine (SASP) group,Wumeiwan (WMW) group, Baitouwengtang (BTWT) group,Senglingbaishusan (SLBSS) group, and Tongxieyaofang (TXYF) group. Each group had 14 rats (with equal ratio of male and female). The six animal model groups of UC-SASP, TXYF, WMW, BTWT, SLBSS, TXYF-were treated by distilled water except the normal control group. Changes of the rat's general conditions after treatment were respectively observed, the colon tissue damage scores were given out, the pathology of colonic mucosa and changes of ultrastructure were analyzed.RESULTS: Different pathological changes on histology were shown after treatment by FRIP. The colon tissue damage score in model group was higher than that of FRIP groups and SASP group (q = 4.59, 4.77, P＜0.05 or q = 5.48,6.25, 5.97, P＜0.01). The scores of WMW group, BTWT group and SLBSS group were lower than that of SASP (q = 4.13, P＜0.05 or q = 5.31, 5.12, P＜0.01). There was no remarkable difference between the damage score of TXYF group and SASP group (q = 3.75, P＞0.05). In addition, some apoptosis cells were found in the pathologic control group.CONCLUSION: The model made with DNCB and acetic acid was successful, and FRIP had better curative effect and WMW was the best curative effect, BTW, SLBSS and TXYF were similar to SASP, and we discovered that apoptosis was possibly related to UC.展开更多
文摘目的:转录因子NF-κB的诱导,调控着免疫和炎症反应中众多基因的表达。溃疡性结肠炎(ulcerative colitis,UC)存在上皮细胞、淋巴细胞、巨噬细胞的异常激活及细胞因子的表达失调。我们检测了UC组织中NF-κBp65,及两种启动子含NF-κB结合位点的蛋白COX-2和iNOS的表达和分布,探讨他们在UC发病机制中的作用,及三者之间的关系。 方法:用免疫组化SP法检测39例活动期溃疡性结肠炎内镜活检标本及30例正常对照石蜡包埋组织中NF-κBp65,COX-2和iNOS的表达情况。 结果:UC组p65,COX-2和iNOS均为阳性表达,主要分布于上皮细胞,固有层炎性细胞及血管内皮细胞也有程度不同的表达。对照组均为阴性或弱阳性表达。三者在UC组的表达与对照组相比,差异均有非常显著性(P<0.01)。p65的表达与内镜及病理分级有关,内镜Ⅱ级与Ⅰ级比较(5.8±2.6 vs 3.6±1.9),差异显著(P<0.05)。病理Ⅱ,Ⅲ级与Ⅰ级比较(6.1±2.4,7.3±2.5 vs 4.0±2.3),差异显著(P<0.05,P<0.01)。iNOS的表达与病理分级有关,Ⅲ级与Ⅰ,Ⅱ级比较(7.8±2.5 vs 4.6±2.3,5.0±1.6),差异显著(P<0.01,P<0.05),COX-2的表达在病情轻重,内镜及病理分级间差异无显著性(p>0.05)。p65与COX-2,iNOS表达显著相关(r_s^1分别为0.713,0.706;P<0.01),COX-2与iNOS表达显著相关(r_s^1=0.
基金Supported by the Hubei Provincial Department of Education, No.99Z014
文摘AIM: To observe different histomorphologic changes of ulcerative colitis (UC) rats that were treated with four regulating-intestine prescriptions (FRIP), to investigate the curative effects of FRIP and to analyze their treatment mechanism.METHODS: The UC rat model was made by the method of 2,4-dinitro chloro benzene (DNCB) immunity and acetic acid local enema. Ninety-eight SD rats were randomly divided into seven groups, namely, the normal control group, model group, salicylazosulfapyridine (SASP) group,Wumeiwan (WMW) group, Baitouwengtang (BTWT) group,Senglingbaishusan (SLBSS) group, and Tongxieyaofang (TXYF) group. Each group had 14 rats (with equal ratio of male and female). The six animal model groups of UC-SASP, TXYF, WMW, BTWT, SLBSS, TXYF-were treated by distilled water except the normal control group. Changes of the rat's general conditions after treatment were respectively observed, the colon tissue damage scores were given out, the pathology of colonic mucosa and changes of ultrastructure were analyzed.RESULTS: Different pathological changes on histology were shown after treatment by FRIP. The colon tissue damage score in model group was higher than that of FRIP groups and SASP group (q = 4.59, 4.77, P＜0.05 or q = 5.48,6.25, 5.97, P＜0.01). The scores of WMW group, BTWT group and SLBSS group were lower than that of SASP (q = 4.13, P＜0.05 or q = 5.31, 5.12, P＜0.01). There was no remarkable difference between the damage score of TXYF group and SASP group (q = 3.75, P＞0.05). In addition, some apoptosis cells were found in the pathologic control group.CONCLUSION: The model made with DNCB and acetic acid was successful, and FRIP had better curative effect and WMW was the best curative effect, BTW, SLBSS and TXYF were similar to SASP, and we discovered that apoptosis was possibly related to UC.
