The human plasma protein binding of water soluble flavonoids in the peels of five spices of citrus fruits was studied by ultrafiltration combined with HPLC.The flavonoids were extracted separately by hot and cold wate...The human plasma protein binding of water soluble flavonoids in the peels of five spices of citrus fruits was studied by ultrafiltration combined with HPLC.The flavonoids were extracted separately by hot and cold water,and higher total flavonoid contents were detected in the former extracts than the latter ones.All the extracts show significant scavenging abilities to both ABTS and DPPH free radicals,which indicates the health benefits of the water extracts of citrus fruits peels.For DPPH radical,the IC50values of hot extract follow as Navel orange(NO)≈Mandarin orange(MO)< Lemon(LE)< Lo tangerine(LO)< Pomelo(PO),while the rank is NO< PO<LE≈MO<LO for ABTS radical.The HPLC results reveal that the kinds and contents of the flavonoids detected in the extracts are different among the species.MO extract has the most neohesperidin dihydrochalcone of 118.76 μmol/L and quercetrin of 211.81 μmol/L of which are much more than the rest extracts.Pomelo extract has the most plentiful flavonoids of naringin with a concentration of 303.28 μmol/L.The high contents of myricetrin and dihydromyricetin which both are potent free radical scavengers may explain the highest free radical scavenging activity of the NO extract.The plasma binding rates decrease with the increasing concentrations of flavonoids,and the flavonoids having plenty hydroxyl groups on both A ring and B ring of the molecular skeleton have relative higher plasma binding rates.In addition,the plasma binding rates of flavonoids with saturated C3-C4 bond decrease significantly with the increasing concentrations.展开更多
Hydroxyl radicals, superoxide anions and nitricoxide radicals are reactive species that can attack biomolecules such as DNA, lipids and proteins to cause many lifestyle-related diseases including hypertension and phot...Hydroxyl radicals, superoxide anions and nitricoxide radicals are reactive species that can attack biomolecules such as DNA, lipids and proteins to cause many lifestyle-related diseases including hypertension and photoaging. This study reports the synthesis of new copper-pyridoxine and iron-pyridoxine complexes. The complexes have been synthesized and characterized by molar conductances, IR, UV-Visible, mass spectrometry, melting points and magnetic moment datas. The molecular formula of the complex is found to be Fe（py ^-）2Cl.H2O. The pyH/pyligand is coordinated to copper and iron through N atom of the pyridine ring and O atom of 5＇-CH2OH group. The Fe（III） complex is found to be paramagnetic with one unpaired electron. The antioxidant activities of the free ligand and its complexes were determined in vitro.展开更多
基金Project(21176263) supported by the National Natural Science Foundation of China
文摘The human plasma protein binding of water soluble flavonoids in the peels of five spices of citrus fruits was studied by ultrafiltration combined with HPLC.The flavonoids were extracted separately by hot and cold water,and higher total flavonoid contents were detected in the former extracts than the latter ones.All the extracts show significant scavenging abilities to both ABTS and DPPH free radicals,which indicates the health benefits of the water extracts of citrus fruits peels.For DPPH radical,the IC50values of hot extract follow as Navel orange(NO)≈Mandarin orange(MO)< Lemon(LE)< Lo tangerine(LO)< Pomelo(PO),while the rank is NO< PO<LE≈MO<LO for ABTS radical.The HPLC results reveal that the kinds and contents of the flavonoids detected in the extracts are different among the species.MO extract has the most neohesperidin dihydrochalcone of 118.76 μmol/L and quercetrin of 211.81 μmol/L of which are much more than the rest extracts.Pomelo extract has the most plentiful flavonoids of naringin with a concentration of 303.28 μmol/L.The high contents of myricetrin and dihydromyricetin which both are potent free radical scavengers may explain the highest free radical scavenging activity of the NO extract.The plasma binding rates decrease with the increasing concentrations of flavonoids,and the flavonoids having plenty hydroxyl groups on both A ring and B ring of the molecular skeleton have relative higher plasma binding rates.In addition,the plasma binding rates of flavonoids with saturated C3-C4 bond decrease significantly with the increasing concentrations.
文摘Hydroxyl radicals, superoxide anions and nitricoxide radicals are reactive species that can attack biomolecules such as DNA, lipids and proteins to cause many lifestyle-related diseases including hypertension and photoaging. This study reports the synthesis of new copper-pyridoxine and iron-pyridoxine complexes. The complexes have been synthesized and characterized by molar conductances, IR, UV-Visible, mass spectrometry, melting points and magnetic moment datas. The molecular formula of the complex is found to be Fe（py ^-）2Cl.H2O. The pyH/pyligand is coordinated to copper and iron through N atom of the pyridine ring and O atom of 5＇-CH2OH group. The Fe（III） complex is found to be paramagnetic with one unpaired electron. The antioxidant activities of the free ligand and its complexes were determined in vitro.
