Objective To explore the effects of SDS, PBS re-dissolvent solutions on fluorescence values of radioallergosorbent test (RAST) inhibition. Methods Dermatophagoides pterronyssinus allergen immunoCAP and UniCAP 100 Syst...Objective To explore the effects of SDS, PBS re-dissolvent solutions on fluorescence values of radioallergosorbent test (RAST) inhibition. Methods Dermatophagoides pterronyssinus allergen immunoCAP and UniCAP 100 System were used. The Sera Pool consisted of 20 Dermatophagoides pterronyssinus allergic patients sera, their specific IgE fluorescence values were between 12 505 and 24 776. Results Fluorescence value percentages decreased: 62.9%, 54.1%, 43.5%, 6.7%, 3.7%, 2.6%, 2.2%, and 1.4% respectively, when SDS concentrations were at 2%, 1%, 0.5%, 0.25%, 0.1%, 0.05%, 0.025%, and 0.01%. Fluorescence values decreased more than 5% with SDS concentrations equal to 0.25% or higher. PBS in 0.1 and 0.01 mol/L concentrations decreased fluorescence values 2.9% and 0.9% respectively. Conclusions SDS is a commonly used surfactants in allergen extract and re-dissolvent prepared allergen precipitation for RAST inhibition. Thus effects of surfactants (e.g. SDS) upon the RAST inhibition tests must be considered when they were used as re-dissolvent agents to improve protein resolution in RAST inhibition.展开更多
The extraction of Eu^(3+)from chloride medium using bifunctional ionic liquid extractants(Bif-ILEs)tri-n-octylmethyl ammonium bis(2-ethyl hexyl)phosphate([A336][D2EHP])and trihexyltetradecyl phosphonium bis(2-ethylhex...The extraction of Eu^(3+)from chloride medium using bifunctional ionic liquid extractants(Bif-ILEs)tri-n-octylmethyl ammonium bis(2-ethyl hexyl)phosphate([A336][D2EHP])and trihexyltetradecyl phosphonium bis(2-ethylhexyl)phosphate([P_(66614)][D2EHP])in kerosene was studied to develop environmentally friendly extraction process.The extraction behavior of Eu^(3+)was examined by varying key process parameters.The extraction behavior indicates that ammonium-based ionic liquid[A336][D2EHP]shows better extraction efficiency of Eu^(3+)than that of phosphonium-based ionic liquid[P_(66614)][D2EHP].Quantitative extraction of Eu^(3+)is obtained with 0.05 mol/L[A336][D2EHP],whereas,0.1 mol/L of[P_(66614)][D2EHP]is required to achieve the same extraction rate of Eu^(3+).The extraction process is endothermic with respect to[A336][D2EHP].Stripping experiments indicate that 100%of Eu^(3+)can be back extracted from both the loaded ionic liquids using 20 vol.%HNO_(3).The highest separation factor(βY/Eu)of 653.59 is reported at pH of 3.2,and the separation factor of Eu^(3+)over La^(3+)is 30.6 at the same pH.From the leach liquors of waste tube light powder,99.98%of Eu and 99.99%Y are recovered using 0.2 mol/L[A336][D2EHP]in 2-stage cross current extraction with aqueous to organcic ratio(O/A)of 1:1 and 1:2.展开更多
We report a novel strategy to study the chain dynamics of poly(acrylic acid) (PAA) in a relative concentrated solution (1.0 g/L). The strategy is based on the fluorescent probe (DCTPE) with unique aggregation-induced ...We report a novel strategy to study the chain dynamics of poly(acrylic acid) (PAA) in a relative concentrated solution (1.0 g/L). The strategy is based on the fluorescent probe (DCTPE) with unique aggregation-induced emission (AIE) characteristics. Free DCTPE molecules are non-emissive in aqueous solution, but they become highly emissive when trapped in polymer coils. The fluorescence intensity is proportional to the efficiency of trapping DCTPE molecules in polymer coils. By correlation the change of fluorescence intensity with the variation of pH value (from 1.78 to 12.06), the PAA chain's dynamics in the relatively concentrated solution have been elucidated into three processes. In the pH range from 12.06 to 6.0, PAA chains take an extended and non-folding conformation. Changing pH from 6.0 to 3.86, PAA chains are partially protonated and loosely packed polymer coils are formed. Further lowering the pH value of the solution (from 3.86 to 1.78), protonated segments dominate the PAA chains, and at the same time, the intermolecular hydrogen bonding takes effect, thus the polymer chains posses in the conformation of more compact coils.展开更多
基金Supported by a grantfrom the Ministryof Science and Technology PRC (2003AA2Z3502 ).
文摘Objective To explore the effects of SDS, PBS re-dissolvent solutions on fluorescence values of radioallergosorbent test (RAST) inhibition. Methods Dermatophagoides pterronyssinus allergen immunoCAP and UniCAP 100 System were used. The Sera Pool consisted of 20 Dermatophagoides pterronyssinus allergic patients sera, their specific IgE fluorescence values were between 12 505 and 24 776. Results Fluorescence value percentages decreased: 62.9%, 54.1%, 43.5%, 6.7%, 3.7%, 2.6%, 2.2%, and 1.4% respectively, when SDS concentrations were at 2%, 1%, 0.5%, 0.25%, 0.1%, 0.05%, 0.025%, and 0.01%. Fluorescence values decreased more than 5% with SDS concentrations equal to 0.25% or higher. PBS in 0.1 and 0.01 mol/L concentrations decreased fluorescence values 2.9% and 0.9% respectively. Conclusions SDS is a commonly used surfactants in allergen extract and re-dissolvent prepared allergen precipitation for RAST inhibition. Thus effects of surfactants (e.g. SDS) upon the RAST inhibition tests must be considered when they were used as re-dissolvent agents to improve protein resolution in RAST inhibition.
文摘The extraction of Eu^(3+)from chloride medium using bifunctional ionic liquid extractants(Bif-ILEs)tri-n-octylmethyl ammonium bis(2-ethyl hexyl)phosphate([A336][D2EHP])and trihexyltetradecyl phosphonium bis(2-ethylhexyl)phosphate([P_(66614)][D2EHP])in kerosene was studied to develop environmentally friendly extraction process.The extraction behavior of Eu^(3+)was examined by varying key process parameters.The extraction behavior indicates that ammonium-based ionic liquid[A336][D2EHP]shows better extraction efficiency of Eu^(3+)than that of phosphonium-based ionic liquid[P_(66614)][D2EHP].Quantitative extraction of Eu^(3+)is obtained with 0.05 mol/L[A336][D2EHP],whereas,0.1 mol/L of[P_(66614)][D2EHP]is required to achieve the same extraction rate of Eu^(3+).The extraction process is endothermic with respect to[A336][D2EHP].Stripping experiments indicate that 100%of Eu^(3+)can be back extracted from both the loaded ionic liquids using 20 vol.%HNO_(3).The highest separation factor(βY/Eu)of 653.59 is reported at pH of 3.2,and the separation factor of Eu^(3+)over La^(3+)is 30.6 at the same pH.From the leach liquors of waste tube light powder,99.98%of Eu and 99.99%Y are recovered using 0.2 mol/L[A336][D2EHP]in 2-stage cross current extraction with aqueous to organcic ratio(O/A)of 1:1 and 1:2.
基金the National Science Foundation of China (21074113)the Key Project of the Natural Science Foundation of Zhejiang Province (Z4110056)
文摘We report a novel strategy to study the chain dynamics of poly(acrylic acid) (PAA) in a relative concentrated solution (1.0 g/L). The strategy is based on the fluorescent probe (DCTPE) with unique aggregation-induced emission (AIE) characteristics. Free DCTPE molecules are non-emissive in aqueous solution, but they become highly emissive when trapped in polymer coils. The fluorescence intensity is proportional to the efficiency of trapping DCTPE molecules in polymer coils. By correlation the change of fluorescence intensity with the variation of pH value (from 1.78 to 12.06), the PAA chain's dynamics in the relatively concentrated solution have been elucidated into three processes. In the pH range from 12.06 to 6.0, PAA chains take an extended and non-folding conformation. Changing pH from 6.0 to 3.86, PAA chains are partially protonated and loosely packed polymer coils are formed. Further lowering the pH value of the solution (from 3.86 to 1.78), protonated segments dominate the PAA chains, and at the same time, the intermolecular hydrogen bonding takes effect, thus the polymer chains posses in the conformation of more compact coils.
