[Objective] This study aimed to investigate the effect of hypertonic solution on the morphology,size,incubation time and cell viability of Mycoplasma hyopneumoniae（Mhp）.[Method] Mhp was stimulated by using NaCl hype...[Objective] This study aimed to investigate the effect of hypertonic solution on the morphology,size,incubation time and cell viability of Mycoplasma hyopneumoniae（Mhp）.[Method] Mhp was stimulated by using NaCl hypertonic solution with a final concentration of 1.8% for 1,2,4,6 and 8 min,respectively.After staining and microscopic examination,the particle size and CCU（color change unit） were determined,and PCR identification was conducted.[Result] After treated with hypertonic solution for different time,Mhp was shrunken and turned round,the particle size was reduced,and the cell number rapidly decreased or even disappeared after 2 min.CCU was reduced by a gradient,and the observation time was extended for 1 d.The target band of Mhp could be amplified from samples after treated with hypertonic solution for different time.After treated with 1.8% NaCl solution for 1-6 min,Mhp changed in the morphology and size but still had viability.[Conclusion] This study provided reference data for exploring the entrance of Mhp into blood circulation after intramuscular injection and a new immunization route of swine Mycoplasma pneumonia vaccine.展开更多
In this paper, we consider the problem (θ(x,U))_t=(K(x,U)U_x)_x-(K(x,U))_x (x,t)∈G_T (θ(x,U)V(x,t))_t=(DθV_x)_x+(V(KU_x-K))_x,(x,t)∈G_T, u(x,0)=u_0(x),V(x,0),(x,0)=V_0(x),0≤x≤2, U(0,t)=h_0(t),U(2,t)=h_2(t),0≤t...In this paper, we consider the problem (θ(x,U))_t=(K(x,U)U_x)_x-(K(x,U))_x (x,t)∈G_T (θ(x,U)V(x,t))_t=(DθV_x)_x+(V(KU_x-K))_x,(x,t)∈G_T, u(x,0)=u_0(x),V(x,0),(x,0)=V_0(x),0≤x≤2, U(0,t)=h_0(t),U(2,t)=h_2(t),0≤t≤T, V(0,t)=g_0(t),V(2,t)=g_2(t),0≤t≤T. Where, θ(x,U)=θ_1(x,U) when (x,t)∈D_1={0≤x<1,0≤t≤T};θ(x,U)=θ_2(x,U),(x,t)∈D_2={1<x≤2,0≤t≤T}.K(x,U)=K_i(x,U),(x,t)∈D_i. θ_i, K_i are the Moisture content and hy draulic conductivity of porous Media on D_i respectively. V be the the concentration of solute in the fluid. In addition we also require that U, V, (K(x,U)U_x-1) and DθV_x+V(KU_x-K) are continu ous at x=1. We prove the exisence, uniqueness and large time behavior of the problem by the method of reg ularization.展开更多
Plasma membrane of plant cells is surrounded by cellulose wall and adjacent cells are joined together by a thick pectin rich matrix. Separation of plant cells and removal of the cell wall experimentally, by either a m...Plasma membrane of plant cells is surrounded by cellulose wall and adjacent cells are joined together by a thick pectin rich matrix. Separation of plant cells and removal of the cell wall experimentally, by either a mechanical or an enzymatic process, results in the production ofprotoplast. Protoplasts are useful tools to study the uptake and transport ofmacromolecules and production of somatic hybrids. Protoplasts can be obtained from all types of actively growing young and healthy tissues. The most convenient and widely used source of plant protoplasts is leaf. Juvenile seedling tissues, cotyledons are other alternative tissues most frequently used for protoplasts isolation. All the environmental and genotypic factors, which affect the cell wall thickenings and compactness indirectly, influence the number of protoplasts recovered. Protoplasts are isolated by two methods, mechanical and enzymatic. The enzyme mixture solution of celluiose/macerozyme is used to digest the cell wall. The critical factors affecting the obtaning ofprotoplasts are the kinds of cell wall degrading enzymes, the physiological state of plant leaves, the type of osmotic stabilizers and the composition of reaction solution. With the improvement of technique and enzyme combination rate, the yield of collected protoplasts will be increased higher.展开更多
An on-chip electroosmotic(EO) micropump(EOP) was integrated in a microfluidic channel combined with a light-addressable potentiometric sensor(LAPS). The movement of EO flow towards right and left directions can be cle...An on-chip electroosmotic(EO) micropump(EOP) was integrated in a microfluidic channel combined with a light-addressable potentiometric sensor(LAPS). The movement of EO flow towards right and left directions can be clearly observed in the microfluidic channel. The characteristics of photocurrent-time and photocurrent-bias voltage are obtained when buffer solution passes through the sensing region. The results demonstrate that the combination of an on-chip EOP with an LAPS is feasible.展开更多
基金Supported by Jiangsu Agricultural Science and Technology Innovation Fund(JASTIF)[CX(10)215]~~
文摘[Objective] This study aimed to investigate the effect of hypertonic solution on the morphology,size,incubation time and cell viability of Mycoplasma hyopneumoniae（Mhp）.[Method] Mhp was stimulated by using NaCl hypertonic solution with a final concentration of 1.8% for 1,2,4,6 and 8 min,respectively.After staining and microscopic examination,the particle size and CCU（color change unit） were determined,and PCR identification was conducted.[Result] After treated with hypertonic solution for different time,Mhp was shrunken and turned round,the particle size was reduced,and the cell number rapidly decreased or even disappeared after 2 min.CCU was reduced by a gradient,and the observation time was extended for 1 d.The target band of Mhp could be amplified from samples after treated with hypertonic solution for different time.After treated with 1.8% NaCl solution for 1-6 min,Mhp changed in the morphology and size but still had viability.[Conclusion] This study provided reference data for exploring the entrance of Mhp into blood circulation after intramuscular injection and a new immunization route of swine Mycoplasma pneumonia vaccine.
文摘In this paper, we consider the problem (θ(x,U))_t=(K(x,U)U_x)_x-(K(x,U))_x (x,t)∈G_T (θ(x,U)V(x,t))_t=(DθV_x)_x+(V(KU_x-K))_x,(x,t)∈G_T, u(x,0)=u_0(x),V(x,0),(x,0)=V_0(x),0≤x≤2, U(0,t)=h_0(t),U(2,t)=h_2(t),0≤t≤T, V(0,t)=g_0(t),V(2,t)=g_2(t),0≤t≤T. Where, θ(x,U)=θ_1(x,U) when (x,t)∈D_1={0≤x<1,0≤t≤T};θ(x,U)=θ_2(x,U),(x,t)∈D_2={1<x≤2,0≤t≤T}.K(x,U)=K_i(x,U),(x,t)∈D_i. θ_i, K_i are the Moisture content and hy draulic conductivity of porous Media on D_i respectively. V be the the concentration of solute in the fluid. In addition we also require that U, V, (K(x,U)U_x-1) and DθV_x+V(KU_x-K) are continu ous at x=1. We prove the exisence, uniqueness and large time behavior of the problem by the method of reg ularization.
文摘Plasma membrane of plant cells is surrounded by cellulose wall and adjacent cells are joined together by a thick pectin rich matrix. Separation of plant cells and removal of the cell wall experimentally, by either a mechanical or an enzymatic process, results in the production ofprotoplast. Protoplasts are useful tools to study the uptake and transport ofmacromolecules and production of somatic hybrids. Protoplasts can be obtained from all types of actively growing young and healthy tissues. The most convenient and widely used source of plant protoplasts is leaf. Juvenile seedling tissues, cotyledons are other alternative tissues most frequently used for protoplasts isolation. All the environmental and genotypic factors, which affect the cell wall thickenings and compactness indirectly, influence the number of protoplasts recovered. Protoplasts are isolated by two methods, mechanical and enzymatic. The enzyme mixture solution of celluiose/macerozyme is used to digest the cell wall. The critical factors affecting the obtaning ofprotoplasts are the kinds of cell wall degrading enzymes, the physiological state of plant leaves, the type of osmotic stabilizers and the composition of reaction solution. With the improvement of technique and enzyme combination rate, the yield of collected protoplasts will be increased higher.
基金supported by the National Natural Science Foundation of China(No.61265006)the China Scholarship Council,and the Graduate School of Biomedical Engineering in Tohoku University
文摘An on-chip electroosmotic(EO) micropump(EOP) was integrated in a microfluidic channel combined with a light-addressable potentiometric sensor(LAPS). The movement of EO flow towards right and left directions can be clearly observed in the microfluidic channel. The characteristics of photocurrent-time and photocurrent-bias voltage are obtained when buffer solution passes through the sensing region. The results demonstrate that the combination of an on-chip EOP with an LAPS is feasible.
