Objective To investigate the molecular mechanism of atherosclerosis that related to age. Methods Immunohistochemistry staining and Western blot were adopted to determine the nuclear translocation of nuclear factor-kap...Objective To investigate the molecular mechanism of atherosclerosis that related to age. Methods Immunohistochemistry staining and Western blot were adopted to determine the nuclear translocation of nuclear factor-kappa B (NF-κB) and expression of platelet-derived growth factor B (PDGF-B) in smooth muscle cells (SMCs) co-cultured with low density lipoprotein (LDL), oxidized LDL (ox-LDL), and ox-LDL+high density lipoprotein (HDL) originated from rats of 2 and 10 months old respectively. Fat stain was used to identify the lipid intake in SMCs. Results The optimal stimulation time of ox-LDL to SMCs was 12 hours. NF-κB intensity increased in most nuclei of SMCs that originated from rats of either 2 or 10 months old co-cultured with ox-LDL. The intensity of NF-κB and the amount of intracellular lipid taken in SMCs were more obvious in cells from 10-month-old rats than from the younger ones. Change of PDGF-B expression in SMCs was not remarkable in each group of rats. Conclusions The 10-month-old rats are more susceptive to ox-LDL than 2-month-old rats in activating nuclear transloca- tion of NF-κB. Maybe this is one of the important reasons contributing to the difference between the older and younger rats on the initiation and development of atherosclerosis lesion. Expression of PDGF-B is not associated with the activity of nuclear translocation of NF-κB.展开更多
The remanufacturing system is remolding the manufacturing industry by bringing scrapped products back to such a condition that reintegrated performance is just as good as new.The remanufacturing environment is feature...The remanufacturing system is remolding the manufacturing industry by bringing scrapped products back to such a condition that reintegrated performance is just as good as new.The remanufacturing environment is featured by a far deeper level of uncertainty than new manufacturing,such as probabilistic routing files,and highly variable processing time.The stochastic disturbances result in the production bottlenecks,which constrain the productivity of the job shop.The uncertainties in the remanufacturing process cause the bottlenecks to shift when the workshop is processing.Considering this outstanding problem,many researchers try to optimize the production process to mitigate dynamic bottlenecks toward a balanced state.This paper proposes a data-driven method to predict bottlenecks in the remanufacturing system with multi-variant uncertainties.Firstly,discrete event simulation technology is applied to establish a simulation model of the remanufacturing production line and calculate the bottleneck index to identify bottlenecks.Secondly,a data-driven method,auto-regressive moving average(ARMA)model is employed to predict the bottlenecks in the system based on real-time data captured by the Arena software.Finally,the proposed prediction method is verified on real data from the automobile engine remanufacturing production line.展开更多
The effective stress method is developed to predict the axial capacity of piles in clay. The effective stress state changes due to the resulting pore pressure change and therefore, the strength and stiffness of the so...The effective stress method is developed to predict the axial capacity of piles in clay. The effective stress state changes due to the resulting pore pressure change and therefore, the strength and stiffness of the soil will change. In this work, the finite element method is utilized as a tool for the analysis of pile-soil systems in undrained condition. The computer program CRISP was developed to suit the problem requirements. CRISP uses the finite element technique and allows predictions to be made of ground deformation using critical state theories. Eight-node isoparametric element was added to the program in addition to the slip element. A pile loading problem was solved in which the pile-soil system is analyzed in undrained condition. The pile is modelled as elastic-plastic material, while the soil is assumed to follow the modified Cam clay model. During undrained loading condition, the settlement values increase by 22% when slip elements are used. The surface settlement increases by about three times when the load is doubled and the surface settlement at all points increases when using slip elements due to the mode of motion which allows smooth movement of the adjacent soil with respect to the pile. The vertical displacement increases as the distance decreases from the pile and negligible values are obtained beyond 10D (where D is the pile diameter) from the center of the pile and these values are slightly increased when slip elements are used. The vertical effective stress along a section at a distance D from the pile center is approximately the same for all load increments and lower values of effective vertical stress can be obtained when slip elements are used.展开更多
Because of the significance to the formation and evolution of the Tibetan plateau, the displacement and slip rate of the Altyn Tagh fault have been topics full of disputation. Scientists who hold different opinions on...Because of the significance to the formation and evolution of the Tibetan plateau, the displacement and slip rate of the Altyn Tagh fault have been topics full of disputation. Scientists who hold different opinions on the evolution of Tibet insist on different slip rates and displacements of the fault zone. In the article, study is focused on the late Quaternary slip rate of the Altyn Tagh fault west of the Cherchen River (between 85°E and 85°45’E). On the basis of high resolution SPOT images of the region, three sites, namely Koramlik, Aqqan pasture and Dalakuansay, were chosen for field investigation. To calculate the slip rate of the fault, displacement of terraces was measured on SPOT satellite images or in situ during fieldwork and thermo-luminescence (TL) dating method was used. To get the ages of terraces, samples of sand were collected from the uppermost sand beds that lie just under loess. The method for calculating slip rate of fault is to divide the displacement of terrace risers by the age of its neighboring lower terrace. The displacement of rivers is not considered in this article because of its uncertainties. At Koramlik, the slip rate of the Altyn Tagh fault is 11.6±2.6mm/a since 6.02±0.47ka B.P and 9.6±2.6mm/a since 15.76±1.19ka B.P. At Aqqan pasture, about 30km west of Koramlik, the slip rate is 12.1±1.9mm/a since 2.06±0.16 ka B.P. At Dalakuansayi, the slip rate of the fault is 12.2±3.0mm/a since 4.91±0.39ka B.P. Hence, we get the average slip rate of 11.4±2.5mm/a for the western part of the Altyn Tagh Fault since Holocene. This result is close to the latest results from GPS research.展开更多
Objective To investigate the depressant effect and mechanism of atorvastatin on the chronic rejection of aortic allograft in rats. Methods： The models of abdominal aorta transplantation were made with micro-surgery i...Objective To investigate the depressant effect and mechanism of atorvastatin on the chronic rejection of aortic allograft in rats. Methods： The models of abdominal aorta transplantation were made with micro-surgery in rats. The recipients were divided into three groups： allograft control group, atorvastatin-treated group and isograft control group. Vascular intimal thickness in all of the groups were observed by histological examination. The expression of PCNA and α-SMA were determined by immunohistochemistry. The content of nitric oxide was determined by nitrate reductase chromatometry. Results： The vascular intimal thickness in rats of atorvastatin-treated group （11.60% ± 2.40% ） were lower than those in allograft control group （34.60 % ± 6.40 % ; P 〈 0.05） and higher than those in isograft control group （1.15 % ± 0.65 %; P〈 0.05 ）. The expression level of PCNA was decreased in atorvastatin-treated group （4.80% ± 0.80% ） than allograft control group （18.40% ± 1.80% ; P〈0.05） and higher than isograft group （1.20% ± 0.40% ; P〈0.05）. Conclusion： The expression of PCNA in the transplant aorta could be suppressed by atorvastatin, which resalted in relief of chronic rejection of aortic allograft.展开更多
文摘Objective To investigate the molecular mechanism of atherosclerosis that related to age. Methods Immunohistochemistry staining and Western blot were adopted to determine the nuclear translocation of nuclear factor-kappa B (NF-κB) and expression of platelet-derived growth factor B (PDGF-B) in smooth muscle cells (SMCs) co-cultured with low density lipoprotein (LDL), oxidized LDL (ox-LDL), and ox-LDL+high density lipoprotein (HDL) originated from rats of 2 and 10 months old respectively. Fat stain was used to identify the lipid intake in SMCs. Results The optimal stimulation time of ox-LDL to SMCs was 12 hours. NF-κB intensity increased in most nuclei of SMCs that originated from rats of either 2 or 10 months old co-cultured with ox-LDL. The intensity of NF-κB and the amount of intracellular lipid taken in SMCs were more obvious in cells from 10-month-old rats than from the younger ones. Change of PDGF-B expression in SMCs was not remarkable in each group of rats. Conclusions The 10-month-old rats are more susceptive to ox-LDL than 2-month-old rats in activating nuclear transloca- tion of NF-κB. Maybe this is one of the important reasons contributing to the difference between the older and younger rats on the initiation and development of atherosclerosis lesion. Expression of PDGF-B is not associated with the activity of nuclear translocation of NF-κB.
基金Projects(51975099,51775086)supported by the Natural Science Foundation of China。
文摘The remanufacturing system is remolding the manufacturing industry by bringing scrapped products back to such a condition that reintegrated performance is just as good as new.The remanufacturing environment is featured by a far deeper level of uncertainty than new manufacturing,such as probabilistic routing files,and highly variable processing time.The stochastic disturbances result in the production bottlenecks,which constrain the productivity of the job shop.The uncertainties in the remanufacturing process cause the bottlenecks to shift when the workshop is processing.Considering this outstanding problem,many researchers try to optimize the production process to mitigate dynamic bottlenecks toward a balanced state.This paper proposes a data-driven method to predict bottlenecks in the remanufacturing system with multi-variant uncertainties.Firstly,discrete event simulation technology is applied to establish a simulation model of the remanufacturing production line and calculate the bottleneck index to identify bottlenecks.Secondly,a data-driven method,auto-regressive moving average(ARMA)model is employed to predict the bottlenecks in the system based on real-time data captured by the Arena software.Finally,the proposed prediction method is verified on real data from the automobile engine remanufacturing production line.
基金Project(RG086/10AET) supported by the Institute of Research Management and Monitoring (IPPP),University of Malaya (UM) under UMRG grant number,Malaysia
文摘The effective stress method is developed to predict the axial capacity of piles in clay. The effective stress state changes due to the resulting pore pressure change and therefore, the strength and stiffness of the soil will change. In this work, the finite element method is utilized as a tool for the analysis of pile-soil systems in undrained condition. The computer program CRISP was developed to suit the problem requirements. CRISP uses the finite element technique and allows predictions to be made of ground deformation using critical state theories. Eight-node isoparametric element was added to the program in addition to the slip element. A pile loading problem was solved in which the pile-soil system is analyzed in undrained condition. The pile is modelled as elastic-plastic material, while the soil is assumed to follow the modified Cam clay model. During undrained loading condition, the settlement values increase by 22% when slip elements are used. The surface settlement increases by about three times when the load is doubled and the surface settlement at all points increases when using slip elements due to the mode of motion which allows smooth movement of the adjacent soil with respect to the pile. The vertical displacement increases as the distance decreases from the pile and negligible values are obtained beyond 10D (where D is the pile diameter) from the center of the pile and these values are slightly increased when slip elements are used. The vertical effective stress along a section at a distance D from the pile center is approximately the same for all load increments and lower values of effective vertical stress can be obtained when slip elements are used.
文摘Because of the significance to the formation and evolution of the Tibetan plateau, the displacement and slip rate of the Altyn Tagh fault have been topics full of disputation. Scientists who hold different opinions on the evolution of Tibet insist on different slip rates and displacements of the fault zone. In the article, study is focused on the late Quaternary slip rate of the Altyn Tagh fault west of the Cherchen River (between 85°E and 85°45’E). On the basis of high resolution SPOT images of the region, three sites, namely Koramlik, Aqqan pasture and Dalakuansay, were chosen for field investigation. To calculate the slip rate of the fault, displacement of terraces was measured on SPOT satellite images or in situ during fieldwork and thermo-luminescence (TL) dating method was used. To get the ages of terraces, samples of sand were collected from the uppermost sand beds that lie just under loess. The method for calculating slip rate of fault is to divide the displacement of terrace risers by the age of its neighboring lower terrace. The displacement of rivers is not considered in this article because of its uncertainties. At Koramlik, the slip rate of the Altyn Tagh fault is 11.6±2.6mm/a since 6.02±0.47ka B.P and 9.6±2.6mm/a since 15.76±1.19ka B.P. At Aqqan pasture, about 30km west of Koramlik, the slip rate is 12.1±1.9mm/a since 2.06±0.16 ka B.P. At Dalakuansayi, the slip rate of the fault is 12.2±3.0mm/a since 4.91±0.39ka B.P. Hence, we get the average slip rate of 11.4±2.5mm/a for the western part of the Altyn Tagh Fault since Holocene. This result is close to the latest results from GPS research.
文摘Objective To investigate the depressant effect and mechanism of atorvastatin on the chronic rejection of aortic allograft in rats. Methods： The models of abdominal aorta transplantation were made with micro-surgery in rats. The recipients were divided into three groups： allograft control group, atorvastatin-treated group and isograft control group. Vascular intimal thickness in all of the groups were observed by histological examination. The expression of PCNA and α-SMA were determined by immunohistochemistry. The content of nitric oxide was determined by nitrate reductase chromatometry. Results： The vascular intimal thickness in rats of atorvastatin-treated group （11.60% ± 2.40% ） were lower than those in allograft control group （34.60 % ± 6.40 % ; P 〈 0.05） and higher than those in isograft control group （1.15 % ± 0.65 %; P〈 0.05 ）. The expression level of PCNA was decreased in atorvastatin-treated group （4.80% ± 0.80% ） than allograft control group （18.40% ± 1.80% ; P〈0.05） and higher than isograft group （1.20% ± 0.40% ; P〈0.05）. Conclusion： The expression of PCNA in the transplant aorta could be suppressed by atorvastatin, which resalted in relief of chronic rejection of aortic allograft.
