Circulating leukocytes in trafficking to the inflammatory sites, will be first tether to, and then roll on the vascular surface. This event is mediated through specific interaction of P-selectin and P-selectin glycopr...Circulating leukocytes in trafficking to the inflammatory sites, will be first tether to, and then roll on the vascular surface. This event is mediated through specific interaction of P-selectin and P-selectin glycoprotein ligand-1 （PSGL-1）, and regulated by hemodynamics. Poor data were reported in understanding P-selectin-mediated rolling. With the flow chamber technique, we herein observed HL-60 cell rolling on P-selectin with or without 3% Ficoll at various wall shear stresses from 0.05 to 0.4 dyn/cm：. The results demonstrated that force rather than transport regulated the rolling, similar to rolling on L- and E-selectin. The rolling was accelerated quickly by an increasing force below the optimal shear threshold of 0.15 dyn/cm2 first and then followed by a slowly decelerating phase starting at the optimum, showing a catch-slip transition and serving as a mechanism for the rolling. The catch-slip transition was completely reflected to the tether lifetime and other rolling parameters, such as the mean and fractional stop time. The narrow catch bond regime stabilized the rolling quickly, through steeply increasing frac- tional stop time to a plateau of about 0.85. Data presented here suggest that the low shear stress threshold serves as a mecha- nism for most cell rolling events through P-selectin.展开更多
A modified discrete element method(DEM)with rolling effect taken into consideration is developed to examine macroscopic behavior of granular materials in this study.Dimensional analysis is firstly performed to establi...A modified discrete element method(DEM)with rolling effect taken into consideration is developed to examine macroscopic behavior of granular materials in this study.Dimensional analysis is firstly performed to establish the relationship between macroscopic mechanical behavior,mesoscale contact parameters at particle level and external loading rate.It is found that only four dimensionless parameters may govern the macroscopic mechanical behavior in bulk.The numerical triaxial apparatus was used to study their influence on the mechanical behavior of granular materials.The parametric study indicates that Poisson’s ratio only varies with stiffness ratio,while Young’s modulus is proportional to contact modulus and grows with stiffness ratio,both of which agree with the micromechanical model.The peak friction angle is dependent on both inter-particle friction angle and rolling resistance.The dilatancy angle relies on inter-particle friction angle if rolling stiffness coefficient is sufficiently large.Finally,we have recommended a calibration procedure for cohesionless soil,which was at once applied to the simulation of Chende sand using a series of triaxial compression tests.The responses of DEM model are shown in quantitative agreement with experiments.In addition,stress-strain response of triaxial extension was also obtained by numerical triaxial extension tests.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.11272125,11072080,31170887 and 31200705)Guangdong Natural Science Foundation(Grant No.S2011010005451)Specialized Research Fund for the Doctoral Program of Higher Education(Grant No.20110172110030)
文摘Circulating leukocytes in trafficking to the inflammatory sites, will be first tether to, and then roll on the vascular surface. This event is mediated through specific interaction of P-selectin and P-selectin glycoprotein ligand-1 （PSGL-1）, and regulated by hemodynamics. Poor data were reported in understanding P-selectin-mediated rolling. With the flow chamber technique, we herein observed HL-60 cell rolling on P-selectin with or without 3% Ficoll at various wall shear stresses from 0.05 to 0.4 dyn/cm：. The results demonstrated that force rather than transport regulated the rolling, similar to rolling on L- and E-selectin. The rolling was accelerated quickly by an increasing force below the optimal shear threshold of 0.15 dyn/cm2 first and then followed by a slowly decelerating phase starting at the optimum, showing a catch-slip transition and serving as a mechanism for the rolling. The catch-slip transition was completely reflected to the tether lifetime and other rolling parameters, such as the mean and fractional stop time. The narrow catch bond regime stabilized the rolling quickly, through steeply increasing frac- tional stop time to a plateau of about 0.85. Data presented here suggest that the low shear stress threshold serves as a mecha- nism for most cell rolling events through P-selectin.
基金supported by the National Natural Science Foundation of China(Grant Nos.11432015 and 10932012)
文摘A modified discrete element method(DEM)with rolling effect taken into consideration is developed to examine macroscopic behavior of granular materials in this study.Dimensional analysis is firstly performed to establish the relationship between macroscopic mechanical behavior,mesoscale contact parameters at particle level and external loading rate.It is found that only four dimensionless parameters may govern the macroscopic mechanical behavior in bulk.The numerical triaxial apparatus was used to study their influence on the mechanical behavior of granular materials.The parametric study indicates that Poisson’s ratio only varies with stiffness ratio,while Young’s modulus is proportional to contact modulus and grows with stiffness ratio,both of which agree with the micromechanical model.The peak friction angle is dependent on both inter-particle friction angle and rolling resistance.The dilatancy angle relies on inter-particle friction angle if rolling stiffness coefficient is sufficiently large.Finally,we have recommended a calibration procedure for cohesionless soil,which was at once applied to the simulation of Chende sand using a series of triaxial compression tests.The responses of DEM model are shown in quantitative agreement with experiments.In addition,stress-strain response of triaxial extension was also obtained by numerical triaxial extension tests.
