Our laboratory seeks to develop minimally invasive cost-effective methods to improve screening and detection of curable precursors to cervical cancer. Previously, we have presented pilot studies that assess the diagno...Our laboratory seeks to develop minimally invasive cost-effective methods to improve screening and detection of curable precursors to cervical cancer. Previously, we have presented pilot studies that assess the diagnostic power of auto-fluorescence and diffuse reflectance spectroscopy. In the present study, we evaluate diffuse reflectance spectra from a comprehensive 850 patient clinical trial to determine its ability to discriminate normal tissue from several grades of abnormal cervical tissue. Methods. Diffuse reflectance spectra at four source detector separations measured from 549 cervical sites were available for analysis. Three classifiers were implemented: one used spectral data directly as input, a second used simple spectral features such as peak position and intensity, and one used principal component analysis for feature selection. Algorithms were developed and evaluated using leave-one-out cross-validation to classify normal and precancerous cervical tissue. The percentage of samples correctly classified was used to evaluate and compare the performance of the algorithms, as compared to histology. Results. Diffuse reflectance spectra of cervical precancer showed consistent differences from that of normal tissue at all source detector separations; reflectance intensity of precancer was lower than that of normal tissue on average. Normal cervical tissue spectra show more intensity variation between patients than other tissue grades. Reflectance spectra acquired from the closest source detector separations consistently demonstrated the most relevant information for tissue classification. Two persistent spectral patterns demonstrated that the contribution of hemoglobin absorption and the wave length-dependent spectral slope contained relevant information for classification. Conclusions. Spectral patterns in diffuse reflectance spectra can be used for the discrimination of normal cervical tissue from lowgrade and high grade squamous intraepithelial lesions.展开更多
O437 96031675散射介质的吸收系数和散射系数的间接测量=Indirectionmeasurement of absorption coefficientsand scattering coefficients for scatteringmedia[刊,中]/沈亦兵(浙江大学光科系.浙江,杭州(310027))//光学仪器.-1995,17(4...O437 96031675散射介质的吸收系数和散射系数的间接测量=Indirectionmeasurement of absorption coefficientsand scattering coefficients for scatteringmedia[刊,中]/沈亦兵(浙江大学光科系.浙江,杭州(310027))//光学仪器.-1995,17(4/5).-69-70介绍了各种测量散射介质光学特性即散射系数和吸收系数的方法。其中蒙特卡罗统计模拟的方法有其独特的优点。对双积分球系统测量散射介质漫反射系数和漫透射系数的原理和双积分球实验系统作了详细的介绍和讨论。(赵桂云)展开更多
文摘Our laboratory seeks to develop minimally invasive cost-effective methods to improve screening and detection of curable precursors to cervical cancer. Previously, we have presented pilot studies that assess the diagnostic power of auto-fluorescence and diffuse reflectance spectroscopy. In the present study, we evaluate diffuse reflectance spectra from a comprehensive 850 patient clinical trial to determine its ability to discriminate normal tissue from several grades of abnormal cervical tissue. Methods. Diffuse reflectance spectra at four source detector separations measured from 549 cervical sites were available for analysis. Three classifiers were implemented: one used spectral data directly as input, a second used simple spectral features such as peak position and intensity, and one used principal component analysis for feature selection. Algorithms were developed and evaluated using leave-one-out cross-validation to classify normal and precancerous cervical tissue. The percentage of samples correctly classified was used to evaluate and compare the performance of the algorithms, as compared to histology. Results. Diffuse reflectance spectra of cervical precancer showed consistent differences from that of normal tissue at all source detector separations; reflectance intensity of precancer was lower than that of normal tissue on average. Normal cervical tissue spectra show more intensity variation between patients than other tissue grades. Reflectance spectra acquired from the closest source detector separations consistently demonstrated the most relevant information for tissue classification. Two persistent spectral patterns demonstrated that the contribution of hemoglobin absorption and the wave length-dependent spectral slope contained relevant information for classification. Conclusions. Spectral patterns in diffuse reflectance spectra can be used for the discrimination of normal cervical tissue from lowgrade and high grade squamous intraepithelial lesions.
文摘O437 96031675散射介质的吸收系数和散射系数的间接测量=Indirectionmeasurement of absorption coefficientsand scattering coefficients for scatteringmedia[刊,中]/沈亦兵(浙江大学光科系.浙江,杭州(310027))//光学仪器.-1995,17(4/5).-69-70介绍了各种测量散射介质光学特性即散射系数和吸收系数的方法。其中蒙特卡罗统计模拟的方法有其独特的优点。对双积分球系统测量散射介质漫反射系数和漫透射系数的原理和双积分球实验系统作了详细的介绍和讨论。(赵桂云)
