This study is part of the endeavor to construct a comprehensive DNA barcoding database for common seaweeds in China. Identifications of red seaweeds, which have simple morphology and anatomy, are sometimes difficult s...This study is part of the endeavor to construct a comprehensive DNA barcoding database for common seaweeds in China. Identifications of red seaweeds, which have simple morphology and anatomy, are sometimes difficult solely depending on morphological characteristics. In recent years, DNA barcode technique has become a more and more effective tool to help solve some of the taxonomic difficulties. Some DNA markers such as COI（cytochrome oxidase subunit I） are proposed as standardized DNA barcodes for all seaweed species. In this study, COI, UPA （universal plastid amplicon, domain V of 23S rRNA）, and ITS （nuclear internal transcribed spacer） were employed to analyze common species of intertidal red seaweeds in Qingdao （119.3°-121°E, 35.35°-37.09°N）. The applicability of using one or a few combined barcodes to identify red seaweed species was tested. The results indicated that COI is a sensitive marker at species level. However, not all the tested species gave PCR amplification products due to lack of the universal primers. The second barcode UPA had effective universal primers but needed to be tested for the effectiveness of resolving closely related species. More than one ITS sequence types were found in some species in this investigation, which might lead to confusion in further analysis. Therefore ITS sequence is not recommended as a universal barcode for seaweeds identification.展开更多
Based on the investigation on shellfish resources from the intertidal zone to subtidal zone of Fenjiezhou Island,Hainan during 2012-2014,the quantity,species,ecological distribution and floristic characters of shellfi...Based on the investigation on shellfish resources from the intertidal zone to subtidal zone of Fenjiezhou Island,Hainan during 2012-2014,the quantity,species,ecological distribution and floristic characters of shellfish from the intertidal zone to subtidal zone of Fenjiezhou Island were analyzed.The results showed that a total of 137 shellfish species were found in Fenjiezhou Island,and they belonged to 4 classes [Polyplacophora（1 species）,Gastropoda（94 species）,Bivalvia（40species）,Cephalopoda（2 species）],13 orders and 44 families.Among the families,Cypraeidae included 11 species,Muricacea included 9 species,Veneracea included9 species,Conidae included 8 species,Strombidae included 6 species,Cymatiidae included 6 species,and the other families all included less than 5 species.According to the characteristics of the components,most of the species belonged to the tropical or subtropical region,and they had a close relationship with coral reef.According to the floristic characters,the shellfish in Fenjiezhou Island belonged to the Indonesia-Malayan Subregion of Indo-West Pacific Region.展开更多
Ataxonomic survey of benthic marine animals from coastal regions of Hainan Island, South China Sea, revealed specimens of a new species of Eunice (Polychaeta: Eunicida: Eunicidae), Eunice uschakovi n. sp., collected f...Ataxonomic survey of benthic marine animals from coastal regions of Hainan Island, South China Sea, revealed specimens of a new species of Eunice (Polychaeta: Eunicida: Eunicidae), Eunice uschakovi n. sp., collected from the intertidal zone. The species belongs to the group of Eunice that has yellow tridentate subacicular hooks and branchiae scattered over an extensive region of the body. It resembles E. miurai and E. havaica in having both bidentate and tridentate falcigers, but can be readily distinguished by branchial features. Comparisons between E. uschakovi and the two related species are presented.展开更多
基金supported by the National Special Research Fund for Non-Profit Sector (Agriculture) (No. 200903030)the National Natural Science Foundation of China (No.41176135)
文摘This study is part of the endeavor to construct a comprehensive DNA barcoding database for common seaweeds in China. Identifications of red seaweeds, which have simple morphology and anatomy, are sometimes difficult solely depending on morphological characteristics. In recent years, DNA barcode technique has become a more and more effective tool to help solve some of the taxonomic difficulties. Some DNA markers such as COI（cytochrome oxidase subunit I） are proposed as standardized DNA barcodes for all seaweed species. In this study, COI, UPA （universal plastid amplicon, domain V of 23S rRNA）, and ITS （nuclear internal transcribed spacer） were employed to analyze common species of intertidal red seaweeds in Qingdao （119.3°-121°E, 35.35°-37.09°N）. The applicability of using one or a few combined barcodes to identify red seaweed species was tested. The results indicated that COI is a sensitive marker at species level. However, not all the tested species gave PCR amplification products due to lack of the universal primers. The second barcode UPA had effective universal primers but needed to be tested for the effectiveness of resolving closely related species. More than one ITS sequence types were found in some species in this investigation, which might lead to confusion in further analysis. Therefore ITS sequence is not recommended as a universal barcode for seaweeds identification.
文摘Based on the investigation on shellfish resources from the intertidal zone to subtidal zone of Fenjiezhou Island,Hainan during 2012-2014,the quantity,species,ecological distribution and floristic characters of shellfish from the intertidal zone to subtidal zone of Fenjiezhou Island were analyzed.The results showed that a total of 137 shellfish species were found in Fenjiezhou Island,and they belonged to 4 classes [Polyplacophora（1 species）,Gastropoda（94 species）,Bivalvia（40species）,Cephalopoda（2 species）],13 orders and 44 families.Among the families,Cypraeidae included 11 species,Muricacea included 9 species,Veneracea included9 species,Conidae included 8 species,Strombidae included 6 species,Cymatiidae included 6 species,and the other families all included less than 5 species.According to the characteristics of the components,most of the species belonged to the tropical or subtropical region,and they had a close relationship with coral reef.According to the floristic characters,the shellfish in Fenjiezhou Island belonged to the Indonesia-Malayan Subregion of Indo-West Pacific Region.
基金Supported by the Ministry of Science and Technology of the People’s Republic of China (MOST) (No. 2006FY110500)the External Cooperation Program from Chinese Academy of Sciences (No. GJHZ200808)
文摘Ataxonomic survey of benthic marine animals from coastal regions of Hainan Island, South China Sea, revealed specimens of a new species of Eunice (Polychaeta: Eunicida: Eunicidae), Eunice uschakovi n. sp., collected from the intertidal zone. The species belongs to the group of Eunice that has yellow tridentate subacicular hooks and branchiae scattered over an extensive region of the body. It resembles E. miurai and E. havaica in having both bidentate and tridentate falcigers, but can be readily distinguished by branchial features. Comparisons between E. uschakovi and the two related species are presented.
