The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (SSH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed d...The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (SSH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed during differentiation and development. In the present study, over 10,000 primary spermatocytes and round spermatid cells were successfully isolated by LCM. Using the cDNAs from primary spermatocytes and round spermatids, SSH cDNAs library of primary spermatocyte-specific was constructed. The average insert size of the cDNA isolated from 75 randomly picked white clones was 500 bp, ranging from 250 bp to 1.7 kb. Using the dot-blot method, a total of 421 clones were examined, resulting in the identification of 390 positive clones emitting strong signals. Partial sequence of cDNAs prepared from each clone was determined with an overall success rate of 84.4%. Genes encoding cytochrome c oxidase II and the rescue factor-humanin were most frequently expressed in primary spermatocytes, suggesting their roles involved in meiosis.展开更多
The interaction of a single three-level trapped ion with two laser beams has been studied theoretically. With application of two successive unitary transformations, an analytical solution to this quantum system has be...The interaction of a single three-level trapped ion with two laser beams has been studied theoretically. With application of two successive unitary transformations, an analytical solution to this quantum system has been obtained.展开更多
We study the dynamics of a two-level trapped ion in a standing wave electromagnetic field in two-dimensional （2D） noncommutative spaces in the Lamb-Dicke regime under the rotating wave approximation. We obtain the ...We study the dynamics of a two-level trapped ion in a standing wave electromagnetic field in two-dimensional （2D） noncommutative spaces in the Lamb-Dicke regime under the rotating wave approximation. We obtain the explicit analytical expressions for the energy spectra, energy eigenstates, unitary time evolution operator, atomic inversion, and phonon number operators. The Rabi oscillations, the collapse, and revivals in the average atomic inversion and the average phonon number are explicitly shown to contain the information of the parameter of the space noncommutativity, which sheds light on proposing new schemes based on the dynamics of trapped ion to test the noncommutativity.展开更多
In this paper, a negatively charged exciton trapped by a spherical parabolic quantum dot has been investigated. The energy spectra of low-lying states are calculated by means of matrix diagonalization. The important f...In this paper, a negatively charged exciton trapped by a spherical parabolic quantum dot has been investigated. The energy spectra of low-lying states are calculated by means of matrix diagonalization. The important feature of the low-lying states of the negatively charged excitons in a spherical quantum dot is obtained via an analysis of the energy spectra.展开更多
在获得光致原子解吸附(light-induced atom desorption,LIAD)效应的基础上,从理论和实验方面分析了LIAD对铯原子磁光阱装载的动力学过程的影响,特别是背景原子对磁光阱的影响.通过实验获得了不同光强和照射时间下关闭解吸附光后磁光阱...在获得光致原子解吸附(light-induced atom desorption,LIAD)效应的基础上,从理论和实验方面分析了LIAD对铯原子磁光阱装载的动力学过程的影响,特别是背景原子对磁光阱的影响.通过实验获得了不同光强和照射时间下关闭解吸附光后磁光阱中铯原子的衰减过程,理论模型定量地描述了背景铯原子造成压强的变化及其对最终平衡态下真空度的影响.该研究对中性原子的长时间俘获,有效控制磁光阱中原子的装载过程具有重要意义.展开更多
文摘The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (SSH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed during differentiation and development. In the present study, over 10,000 primary spermatocytes and round spermatid cells were successfully isolated by LCM. Using the cDNAs from primary spermatocytes and round spermatids, SSH cDNAs library of primary spermatocyte-specific was constructed. The average insert size of the cDNA isolated from 75 randomly picked white clones was 500 bp, ranging from 250 bp to 1.7 kb. Using the dot-blot method, a total of 421 clones were examined, resulting in the identification of 390 positive clones emitting strong signals. Partial sequence of cDNAs prepared from each clone was determined with an overall success rate of 84.4%. Genes encoding cytochrome c oxidase II and the rescue factor-humanin were most frequently expressed in primary spermatocytes, suggesting their roles involved in meiosis.
基金The project supported by the Natural Science Foundation of Education Committee of Anhui Province of China under Grant No. 2004kj186
文摘The interaction of a single three-level trapped ion with two laser beams has been studied theoretically. With application of two successive unitary transformations, an analytical solution to this quantum system has been obtained.
基金The project supported by National Natural Science Foundation of China under Grant Nos. 10575040, 90503010, 60478029, and 10634060, and the State Key Basic Research Program of China under Grant No. 2005CB724508
文摘We study the dynamics of a two-level trapped ion in a standing wave electromagnetic field in two-dimensional （2D） noncommutative spaces in the Lamb-Dicke regime under the rotating wave approximation. We obtain the explicit analytical expressions for the energy spectra, energy eigenstates, unitary time evolution operator, atomic inversion, and phonon number operators. The Rabi oscillations, the collapse, and revivals in the average atomic inversion and the average phonon number are explicitly shown to contain the information of the parameter of the space noncommutativity, which sheds light on proposing new schemes based on the dynamics of trapped ion to test the noncommutativity.
基金The project supported by National Natural Science Foundation of China under Grant No. 10475021 and the Natured Science Foundation of Guangdong Province of China under Grant No. 04009519
文摘In this paper, a negatively charged exciton trapped by a spherical parabolic quantum dot has been investigated. The energy spectra of low-lying states are calculated by means of matrix diagonalization. The important feature of the low-lying states of the negatively charged excitons in a spherical quantum dot is obtained via an analysis of the energy spectra.
文摘在获得光致原子解吸附(light-induced atom desorption,LIAD)效应的基础上,从理论和实验方面分析了LIAD对铯原子磁光阱装载的动力学过程的影响,特别是背景原子对磁光阱的影响.通过实验获得了不同光强和照射时间下关闭解吸附光后磁光阱中铯原子的衰减过程,理论模型定量地描述了背景铯原子造成压强的变化及其对最终平衡态下真空度的影响.该研究对中性原子的长时间俘获,有效控制磁光阱中原子的装载过程具有重要意义.
