To perceive the epithelial dysplasia from the light scattering spectroscopy (LSS) is an inverse problem, which can be transformed into the inversion of the size distribution of epithelial-cell nuclei. Based on the sim...To perceive the epithelial dysplasia from the light scattering spectroscopy (LSS) is an inverse problem, which can be transformed into the inversion of the size distribution of epithelial-cell nuclei. Based on the simulation of single polarized LSS for epithelial-cell nuclei, Chahine algorithm is adopted to retrieve the size distribution. Numerical results show that Chahine algorithm has high inversion precision for both single-peaked and bimodal models, which implies the potential to increase diagnostic resolution of LSS.展开更多
Objective To evaluate the predictive factors of refractory glaucoma treated with diode laser transscleral cyclophotocoagulation(TSCP) in a midterm observation.Methods Fifty-four consecutive patients(54 eyes) with refr...Objective To evaluate the predictive factors of refractory glaucoma treated with diode laser transscleral cyclophotocoagulation(TSCP) in a midterm observation.Methods Fifty-four consecutive patients(54 eyes) with refractory glaucoma were enrolled and underwent TSCP(power of 1.5-2.0 W,exposure time of 2 seconds and 20-50 applications).Patients were regularly followed up for 12 months.Intraocular pressure(IOP) was measured before TSCP treatment and at each follow-up visit.Patients were divided into the sensitive and insensitive groups according to the effect of a single treatment of TSCP on IOP.The success rate was defined as the percentage of eyes achieving an IOP between 5 and 21 mm Hg following TSCP therapy at their final follow up visit.Difference of age between the sensitive and insensitive groups was evaluated with Independent-samples T test and that of gender distribution of two groups was evaluated with Fisher's exact test.The success rate of different types of glaucoma to TSCP was calculated.Results A total of 32 patients were sensitive to TSCP(sensitive group) and 22 patients were insensitive to TSCP(insensitive group).The success rates of female(77.8%,14/18) and cases being over 50 years(71.4%,20/28) were higher than those of male(50.0%,18/36) and ones being under 50 years(46.2%,12/26)(all P>0.05).The age of the sensitive and insensitive groups was 56.0±15.5 and 46.4±15.4 years,respectively(P=0.029).The age(P=0.029) and gender distribution(P=0.046) of the two groups had significant difference.The success rate of neovascular glaucoma,primary angle-closure glaucoma,primary open angle glaucoma,traumatic glaucoma,uveitic glaucoma,and secondary glaucoma after silicone oil injection to TSCP was 57.1%,100.0%,50.0%,20.0%,81.8%,and 0%,respectively.Conclusion Age,gender,and type of glaucoma appear to be associated with effect of TSCP on IOP control.展开更多
The localized surface plasmon resonance of gold nanoparticles enables them to be excellent light scattering sensing reagents and efficient lignt-heat convertors for potential diagnostics and therapeutics.In this work,...The localized surface plasmon resonance of gold nanoparticles enables them to be excellent light scattering sensing reagents and efficient lignt-heat convertors for potential diagnostics and therapeutics.In this work,gold nanoparticles of 15 nm in size were synthesized and conjugated with a kind of arginine-glycine-aspartic acid peptide(RGD)to target the cancer cells.Under a conventional dark field microscope,the scattering images from normal and cancer cells are very different.Only a few of gold nanoparticles bind to the membrane of normal cells due to nonspecific interaction.Yet for cancer cells,the concentration of gold nanoparticles inside cancer cells is much higher than that inside normal cells,showing a strong light scattering signal.By exposing these cells to an external laser irradiation,cancer cells were selectively destructed because of the heat conversion of gold nanoparticles inside them,but normal cells still kept alive due to the absence of gold nanoparticles in them.These results incidated that gold nanoparticles might be potential contrast reagents for targeted cancer imaging and laser therapy.展开更多
Glucose oxidase(GOx)-based nanotheranostic agents hold great promise in tumor starvation and its synergistic therapy. Self-assembled plasmonic gold vesicles(GVs) with unique optical properties, large hollow cavity, an...Glucose oxidase(GOx)-based nanotheranostic agents hold great promise in tumor starvation and its synergistic therapy. Self-assembled plasmonic gold vesicles(GVs) with unique optical properties, large hollow cavity, and strong localized surface plasmon resonance, can be used as multi-functional nanocarriers for synergistic therapy. Herein,GOx-loaded GVs(GV-GOx) were developed for light-triggered GOx release as well as enhanced catalytic activity of GOx, achieving programmable photothermal/starvation therapy. Under near-infrared laser irradiation, the GV-GOx generated strong localized hyperthermia due to plasmon coupling effect of GVs, promoting the release of encapsulated GOx and increasing its catalytic activity, resulting in enhanced tumor starvation effect. In addition, the high photothermal effect improved the cellular uptake of GV-GOx and allowed an efficient monitoring of synergistic tumor treatment via photoacoustic/photothermal duplex imaging in vivo. Impressively, the synergistic photothermal/starvation therapy demonstrated complete tumor eradication in 4 T1 tumorbearing mice, verifying superior synergistic anti-tumor therapeutic effects than monotherapy with no apparent systemic side effects. Our work demonstrated the development of a light-triggered nanoplatform for cancer synergistic therapy.展开更多
基金Supported partially by the Young Scholar Foundation of NanjingUniversity of Science and Technology(Grant No. NJUST200204)
文摘To perceive the epithelial dysplasia from the light scattering spectroscopy (LSS) is an inverse problem, which can be transformed into the inversion of the size distribution of epithelial-cell nuclei. Based on the simulation of single polarized LSS for epithelial-cell nuclei, Chahine algorithm is adopted to retrieve the size distribution. Numerical results show that Chahine algorithm has high inversion precision for both single-peaked and bimodal models, which implies the potential to increase diagnostic resolution of LSS.
文摘Objective To evaluate the predictive factors of refractory glaucoma treated with diode laser transscleral cyclophotocoagulation(TSCP) in a midterm observation.Methods Fifty-four consecutive patients(54 eyes) with refractory glaucoma were enrolled and underwent TSCP(power of 1.5-2.0 W,exposure time of 2 seconds and 20-50 applications).Patients were regularly followed up for 12 months.Intraocular pressure(IOP) was measured before TSCP treatment and at each follow-up visit.Patients were divided into the sensitive and insensitive groups according to the effect of a single treatment of TSCP on IOP.The success rate was defined as the percentage of eyes achieving an IOP between 5 and 21 mm Hg following TSCP therapy at their final follow up visit.Difference of age between the sensitive and insensitive groups was evaluated with Independent-samples T test and that of gender distribution of two groups was evaluated with Fisher's exact test.The success rate of different types of glaucoma to TSCP was calculated.Results A total of 32 patients were sensitive to TSCP(sensitive group) and 22 patients were insensitive to TSCP(insensitive group).The success rates of female(77.8%,14/18) and cases being over 50 years(71.4%,20/28) were higher than those of male(50.0%,18/36) and ones being under 50 years(46.2%,12/26)(all P>0.05).The age of the sensitive and insensitive groups was 56.0±15.5 and 46.4±15.4 years,respectively(P=0.029).The age(P=0.029) and gender distribution(P=0.046) of the two groups had significant difference.The success rate of neovascular glaucoma,primary angle-closure glaucoma,primary open angle glaucoma,traumatic glaucoma,uveitic glaucoma,and secondary glaucoma after silicone oil injection to TSCP was 57.1%,100.0%,50.0%,20.0%,81.8%,and 0%,respectively.Conclusion Age,gender,and type of glaucoma appear to be associated with effect of TSCP on IOP control.
基金supported by the National Natural Science Foundation of China(Grant No.81071256)the Natural Science Foundation of Jiangsu Province(Grant No.BK2008400)the Startup Foundation of NUAA
文摘The localized surface plasmon resonance of gold nanoparticles enables them to be excellent light scattering sensing reagents and efficient lignt-heat convertors for potential diagnostics and therapeutics.In this work,gold nanoparticles of 15 nm in size were synthesized and conjugated with a kind of arginine-glycine-aspartic acid peptide(RGD)to target the cancer cells.Under a conventional dark field microscope,the scattering images from normal and cancer cells are very different.Only a few of gold nanoparticles bind to the membrane of normal cells due to nonspecific interaction.Yet for cancer cells,the concentration of gold nanoparticles inside cancer cells is much higher than that inside normal cells,showing a strong light scattering signal.By exposing these cells to an external laser irradiation,cancer cells were selectively destructed because of the heat conversion of gold nanoparticles inside them,but normal cells still kept alive due to the absence of gold nanoparticles in them.These results incidated that gold nanoparticles might be potential contrast reagents for targeted cancer imaging and laser therapy.
基金supported by the National Natural Science Foundation of China (31771036 and 51703132)the Basic Research Program of Shenzhen (JCYJ20180507182413022 and JCYJ20170412111100742)+1 种基金Guangdong Province Natural Science Foundation of Major Basic Research and Cultivation Project(2018B030308003)Fok Ying-Tong Education Foundation for Young Teachers in the Higher Education Institutions of China (161032)。
文摘Glucose oxidase(GOx)-based nanotheranostic agents hold great promise in tumor starvation and its synergistic therapy. Self-assembled plasmonic gold vesicles(GVs) with unique optical properties, large hollow cavity, and strong localized surface plasmon resonance, can be used as multi-functional nanocarriers for synergistic therapy. Herein,GOx-loaded GVs(GV-GOx) were developed for light-triggered GOx release as well as enhanced catalytic activity of GOx, achieving programmable photothermal/starvation therapy. Under near-infrared laser irradiation, the GV-GOx generated strong localized hyperthermia due to plasmon coupling effect of GVs, promoting the release of encapsulated GOx and increasing its catalytic activity, resulting in enhanced tumor starvation effect. In addition, the high photothermal effect improved the cellular uptake of GV-GOx and allowed an efficient monitoring of synergistic tumor treatment via photoacoustic/photothermal duplex imaging in vivo. Impressively, the synergistic photothermal/starvation therapy demonstrated complete tumor eradication in 4 T1 tumorbearing mice, verifying superior synergistic anti-tumor therapeutic effects than monotherapy with no apparent systemic side effects. Our work demonstrated the development of a light-triggered nanoplatform for cancer synergistic therapy.
