目的:探讨海马CA1锥体神经元对不同单色激光闪光刺激的反应,为论证激光诱发的神经系统生物学效应提供实验支持。方法:健康成年SD大鼠麻醉后,行气管插管术,开颅钻孔暴露脑面,进行在体膜片钳记录。全细胞记录稳定后,采用4种不同波长的单...目的:探讨海马CA1锥体神经元对不同单色激光闪光刺激的反应,为论证激光诱发的神经系统生物学效应提供实验支持。方法:健康成年SD大鼠麻醉后,行气管插管术,开颅钻孔暴露脑面,进行在体膜片钳记录。全细胞记录稳定后,采用4种不同波长的单色激光对其眼球进行闪光刺激,记录大鼠海马神经元的反应。结果:给予大鼠闪光刺激后,其海马神经元对蓝色激光和紫色激光表现出明显的超极化反应,对红色和绿色激光刺激则没有明显的反应。蓝光引起的变化中,超极化的幅值为(8.32±1.10)m V,反应持续时间为(115.32±13.02)ms;紫光引起的超极化的幅值为(9.01±2.25)m V,刺激反应持续时间为(109.27±16.62)ms,蓝光和紫光之间没有统计学差异。50 m W、75 m W、100 m W、125 m W功率的紫色激光刺激引起的超极化幅值,分别为(7.28±0.16)m V、(9.25±0.71)m V、(10.91±0.08)m V、(12.67±0.38)m V,相关性分析显示幅值变化与刺激功率高度正相关。结论:麻醉状态下,蓝光和紫色的闪光刺激可以诱导大鼠海马神经元出现明确的抑制性反应,红光和绿光没有明显的作用。展开更多
Q631 99042356激光辐照在优良根霉菌株选育中的应用研究=Applicationstudy of laser irradiation in good rhizogillusstrain selection[刊,中]/王瑞丽,欧家鸣,尚鹤龄,杨卫平,周汝敏,许坤一,余尊祥(云南师范大学.云南,昆明(650092))//...Q631 99042356激光辐照在优良根霉菌株选育中的应用研究=Applicationstudy of laser irradiation in good rhizogillusstrain selection[刊,中]/王瑞丽,欧家鸣,尚鹤龄,杨卫平,周汝敏,许坤一,余尊祥(云南师范大学.云南,昆明(650092))//激光杂志.—1998,19(4).展开更多
The coil-to-globule transition of thermally sensitive linear poly(N-isopropylacrylamide) (PNIPAM) labeled with dansyl group is induced by 1.54 μm laser pulses (widths10 ns). The dansyl group is used to follow t...The coil-to-globule transition of thermally sensitive linear poly(N-isopropylacrylamide) (PNIPAM) labeled with dansyl group is induced by 1.54 μm laser pulses (widths10 ns). The dansyl group is used to follow the transition kinetics because its fluorescence intensity is very sensitive to its micro-environment. As the molar ratio of NIPAM monomer to dansyl group increases from 110 to 300, the effect of covalently attached dansyl fluorophores on the transition decreases. In agreement with our previous study in which we used 8-anilino- l-naphthalensulfonic acid ammonium salt free in water as a fluorescent probe, the current study reveals that the transition has two distinct stages with two characteristic times, namely, Tfast≈0.1 ms, which can be attributed to the nucleation and formation of some "pearls" (locally contracting segments) on the chain, and tslow≈0.5 ms, which is related to the merging and coarsening of the "pearls".Tfast is independent of the PNIPAM chain length over a wide range (Mw=2.8× 10^6-4.2 × 10^7 g/mol). On the other hand, Tslow only slightly increases with the chain length.展开更多
This study investigated the effects of clothing fabric on human skin microcirculation.Once skin is covered with a clothing fabric,human sensations,namely,coolness,warmth,softness,and roughness,are aroused immediately,...This study investigated the effects of clothing fabric on human skin microcirculation.Once skin is covered with a clothing fabric,human sensations,namely,coolness,warmth,softness,and roughness,are aroused immediately,and the cutaneous microcirculation may be changed consequently.Since the complex relationships of the human skin,the environment,and the clothing,there is few publication focusing on the physiological responses of the skin to the fabrics.In this paper,a Laser Doppler Flowmetry(LDF)was used to test the dynamic responses of the skin blood flow when the fabric was placed on the skin.Effects of different fabrics on the skin blood flux were investigated.The results show that cold stimulation of fabric has remarkable influences on the skin blood flux,and the surface properties of fabric are of importance to affect the human skin blood flow.展开更多
文摘目的:探讨海马CA1锥体神经元对不同单色激光闪光刺激的反应,为论证激光诱发的神经系统生物学效应提供实验支持。方法:健康成年SD大鼠麻醉后,行气管插管术,开颅钻孔暴露脑面,进行在体膜片钳记录。全细胞记录稳定后,采用4种不同波长的单色激光对其眼球进行闪光刺激,记录大鼠海马神经元的反应。结果:给予大鼠闪光刺激后,其海马神经元对蓝色激光和紫色激光表现出明显的超极化反应,对红色和绿色激光刺激则没有明显的反应。蓝光引起的变化中,超极化的幅值为(8.32±1.10)m V,反应持续时间为(115.32±13.02)ms;紫光引起的超极化的幅值为(9.01±2.25)m V,刺激反应持续时间为(109.27±16.62)ms,蓝光和紫光之间没有统计学差异。50 m W、75 m W、100 m W、125 m W功率的紫色激光刺激引起的超极化幅值,分别为(7.28±0.16)m V、(9.25±0.71)m V、(10.91±0.08)m V、(12.67±0.38)m V,相关性分析显示幅值变化与刺激功率高度正相关。结论:麻醉状态下,蓝光和紫色的闪光刺激可以诱导大鼠海马神经元出现明确的抑制性反应,红光和绿光没有明显的作用。
文摘Q631 99042356激光辐照在优良根霉菌株选育中的应用研究=Applicationstudy of laser irradiation in good rhizogillusstrain selection[刊,中]/王瑞丽,欧家鸣,尚鹤龄,杨卫平,周汝敏,许坤一,余尊祥(云南师范大学.云南,昆明(650092))//激光杂志.—1998,19(4).
文摘The coil-to-globule transition of thermally sensitive linear poly(N-isopropylacrylamide) (PNIPAM) labeled with dansyl group is induced by 1.54 μm laser pulses (widths10 ns). The dansyl group is used to follow the transition kinetics because its fluorescence intensity is very sensitive to its micro-environment. As the molar ratio of NIPAM monomer to dansyl group increases from 110 to 300, the effect of covalently attached dansyl fluorophores on the transition decreases. In agreement with our previous study in which we used 8-anilino- l-naphthalensulfonic acid ammonium salt free in water as a fluorescent probe, the current study reveals that the transition has two distinct stages with two characteristic times, namely, Tfast≈0.1 ms, which can be attributed to the nucleation and formation of some "pearls" (locally contracting segments) on the chain, and tslow≈0.5 ms, which is related to the merging and coarsening of the "pearls".Tfast is independent of the PNIPAM chain length over a wide range (Mw=2.8× 10^6-4.2 × 10^7 g/mol). On the other hand, Tslow only slightly increases with the chain length.
基金Key Science and Technology Project of Tianjin Municipal Science and Technology Commission,China(No.09JCZDJC24400)
文摘This study investigated the effects of clothing fabric on human skin microcirculation.Once skin is covered with a clothing fabric,human sensations,namely,coolness,warmth,softness,and roughness,are aroused immediately,and the cutaneous microcirculation may be changed consequently.Since the complex relationships of the human skin,the environment,and the clothing,there is few publication focusing on the physiological responses of the skin to the fabrics.In this paper,a Laser Doppler Flowmetry(LDF)was used to test the dynamic responses of the skin blood flow when the fabric was placed on the skin.Effects of different fabrics on the skin blood flux were investigated.The results show that cold stimulation of fabric has remarkable influences on the skin blood flux,and the surface properties of fabric are of importance to affect the human skin blood flow.