AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared...AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared and investigated. METHODS: An immunostimulating complexes(ISCOMS)-based vaccine containing a novel therapeutic hepatitis B polypeptide was prepared by dialysis method, and its formation was visualized by electron microscopy and biochemically verified by SDS-polyacrylamide gel electrophoresis. Amount of the peptide within ISCOMS was determined by Bradford assay, and specific CTL response was detected by ELISPOT assay. RESULTS: Typical cage-like structures of submicroparticle with a diameter of about 40nm were observed by electron microscopy. Results from Bradford assay showed that the level of peptide incorporation was about 0.33g.L(-1). At the paralleled position close to the sixth band of the molecular weight marker(3480kDa) a clear band was shown in SDS-PAGE analysis, indicating successful incorporation of polypeptide into ISCOMS. It is suggested that ISCOMS delivery system could efficiently improve the immunogenicity of polypeptide and elicit specific immune responses in vivo by the results of ELISPOT assay, which showed that IFN-gamma producing cells(specific CTL responses) were increased(spots of ISCOMS-treated group: 47+/-5, n =3; control group: 5+/-2, n =3). CONCLUSION: ISCOMS-based hepatitis B polypeptide vaccine is successfully constructed and it induces a higher CTL response compared with short polypeptides vaccine in vivo.展开更多
The bilayer organic light emitting devices （OLEDs） using two common aromatic diamines as hole transporting materials （HTMs） and BBOT （2,5-bis（5-tert-butyl-2-benzoxazolyl）thiophene） as electron transporting mat...The bilayer organic light emitting devices （OLEDs） using two common aromatic diamines as hole transporting materials （HTMs） and BBOT （2,5-bis（5-tert-butyl-2-benzoxazolyl）thiophene） as electron transporting material have been prepared, in which the electroluminescent spectra are different from the fluorescent spectra of each of the constituent materials. The electroluminescence is mainly attributed to exciplex confirmed by photoluminescence and electroluminescence measurements, and the type of exicplex is deternfined in terms of the energy level diagram of the bilayer devices, By comparing the molecular structures and energy levels of TPD and NPB, it is demonstrated that the structure of a molecule as well as its energy level has an effect on the exciplex formation.展开更多
基金the National Natural Science Foundation of China,No.39789010
文摘AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared and investigated. METHODS: An immunostimulating complexes(ISCOMS)-based vaccine containing a novel therapeutic hepatitis B polypeptide was prepared by dialysis method, and its formation was visualized by electron microscopy and biochemically verified by SDS-polyacrylamide gel electrophoresis. Amount of the peptide within ISCOMS was determined by Bradford assay, and specific CTL response was detected by ELISPOT assay. RESULTS: Typical cage-like structures of submicroparticle with a diameter of about 40nm were observed by electron microscopy. Results from Bradford assay showed that the level of peptide incorporation was about 0.33g.L(-1). At the paralleled position close to the sixth band of the molecular weight marker(3480kDa) a clear band was shown in SDS-PAGE analysis, indicating successful incorporation of polypeptide into ISCOMS. It is suggested that ISCOMS delivery system could efficiently improve the immunogenicity of polypeptide and elicit specific immune responses in vivo by the results of ELISPOT assay, which showed that IFN-gamma producing cells(specific CTL responses) were increased(spots of ISCOMS-treated group: 47+/-5, n =3; control group: 5+/-2, n =3). CONCLUSION: ISCOMS-based hepatitis B polypeptide vaccine is successfully constructed and it induces a higher CTL response compared with short polypeptides vaccine in vivo.
文摘The bilayer organic light emitting devices （OLEDs） using two common aromatic diamines as hole transporting materials （HTMs） and BBOT （2,5-bis（5-tert-butyl-2-benzoxazolyl）thiophene） as electron transporting material have been prepared, in which the electroluminescent spectra are different from the fluorescent spectra of each of the constituent materials. The electroluminescence is mainly attributed to exciplex confirmed by photoluminescence and electroluminescence measurements, and the type of exicplex is deternfined in terms of the energy level diagram of the bilayer devices, By comparing the molecular structures and energy levels of TPD and NPB, it is demonstrated that the structure of a molecule as well as its energy level has an effect on the exciplex formation.
