期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
光照对马岗鹅卵泡发育及相关激素mRNA水平的影响 被引量:9
1
作者 刘容珍 黄运茂 +2 位作者 李万利 田允波 施振旦 《华南农业大学学报》 CAS CSCD 北大核心 2009年第3期76-80,共5页
研究了光照对马岗鹅卵泡发育及相关激素基因表达的影响,试验期对照组接受短光照(11 h光∶13 h暗),处理组在接受7 d短光照后接受长光照(16 h光∶8 h暗).结果表明在整个试验期,对照组产蛋率维持10%以上,处理组则在接受长光照约15 d后产蛋... 研究了光照对马岗鹅卵泡发育及相关激素基因表达的影响,试验期对照组接受短光照(11 h光∶13 h暗),处理组在接受7 d短光照后接受长光照(16 h光∶8 h暗).结果表明在整个试验期,对照组产蛋率维持10%以上,处理组则在接受长光照约15 d后产蛋率快速下降并于试验第55 d停产.在处理组接受长光照17 d(试验第25 d)后,与对照组相比,其下丘脑血管活性肠肽(VIP)和垂体催乳素(PRL)的基因表达稍提高(P>0.05),血浆PRL水平明显高于对照组(P<0.05),而促黄体素(LH)水平和卵巢大黄卵泡(LYF)数、小黄卵泡(SYF)数则无差异,仅大白卵泡(LWF)数显著低于后者(P<0.01);在处理组接受长光照45 d后(试验第53 d),其下丘脑VIP和垂体PRL的基因表达显著提高(P<0.01),且血浆PRL质量浓度显著高于对照组(P<0.01),LH水平则明显低于对照组(P<0.05),卵巢上无任何卵泡,对照组的LYF、SYF和LWF数与试验第25 d时相似.整个试验期,长光照对下丘脑促性腺激素释放激素(GnRH)和垂体LHβ的基因表达均无明显影响.表明长光照能促进下丘脑VIP和垂体PRL的基因表达,提高血浆PRL水平和抑制卵泡发育. 展开更多
关键词 马岗鹅 光照 卵泡发育 激素mrna水平
下载PDF
Quantitative profiles of the mRNAs of ER-α and its novel variant ER-α36 in breast cancers and matched normal tissues 被引量:2
2
作者 Yi ZHENG Jing ZHANG +7 位作者 Zhen-zhen XU Jian-ming SHENG Xiao-chen ZHANG Hao-hao WANG Xiao-dong TENG Xiao-jiao LIU Jiang CAO Li-song TENG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第2期144-150,共7页
Objective: The novel estrogen receptor-α (ER-α) variant ER-α36 is reported to be functional in the es-trogen signaling pathway and is related to tamoxifen resistance in breast cancer. However, ER-α36 tends to be a... Objective: The novel estrogen receptor-α (ER-α) variant ER-α36 is reported to be functional in the es-trogen signaling pathway and is related to tamoxifen resistance in breast cancer. However, ER-α36 tends to be a favorable factor for survival in patients without tamoxifen therapy. To investigate the mechanisms behind this paradox, we determined the differences between the transcriptional profiles of ER-α36 and full-length ER-α (ER-α66) in breast cancers and matched normal tissues. Methods: We analyzed ER-α36 and ER-α66 messenger RNA ( mRNA) levels in 74 pairs of breast cancers and matched normal tissues using a real-time quantitative polymerase chain reaction (PCR) assay, and correlated the results with their clinicopathological characteristics. Results: Breast cancers expressed lower ER-α36 mRNA levels than matched normal tissues regardless of their ER-α66 expression status. Down-regulation of ER-α36 mRNA was correlated with local progression, lymph node metastasis, and advanced cancer stage. The level of ER-α66 mRNA was lower in ER-α negative breast cancers compared with matched normal tissues. No differences in ER-α66 mRNA levels were observed during cancer progression. Conclusion: Down-regulation of ER-α36 is associated with carcinogenesis and progression of breast cancer. 展开更多
关键词 Breast cancer Estrogen receptor Estrogen receptor-α36 (ER-α36) ER-α66
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部