Objective: To determine the protective effect of monosialoganglionside (GM1) and evaluate the influence of GM1 on expression of N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in Sprague-Dawley (SD) rats with focal c...Objective: To determine the protective effect of monosialoganglionside (GM1) and evaluate the influence of GM1 on expression of N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in Sprague-Dawley (SD) rats with focal cerebral ische- mia-reperfusion (I/R). Methods: Left middle cerebral artery (MCA) was occluded by an intraluminal suture for 1 h and the brain was reperfused for 72 h in SD rats when infarct volume was measured, GM1 (10 mg/kg) was given ip (intraperitoneally) at 5 min (group A), 1 h (group B) and 2 h (group C) after MCA occlusion (MCAo). Expression of NMDAR1 was detected by Western blot at various time after reperfusion (4 h, 6 h, 24 h, 48 h and 72 h) in ischemic hemispheres of the rats with or without GM1 admin- istered. Results: (1) Adjusted relative infarct volumes of groups A and B were significantly smaller than that of group C and the control group (P<0.01 and P<0.05, respectively). (2) Expression level of NMDAR1 was temporally high at 6 h after reperfusion, and dipped below the normal level at 72 h after reperfusion. GM1 at 5 min after MCAo significantly suppressed the expression of NMDAR1 at 6 h after reperfusion (P<0.05 vs the control). At 72 h after reperfusion, the NMDAR1 expression level of rats treated with GM1 administered (at 5 min or 2 h after MCAo) was significantly higher than that of the control (P<0.05). Conclusion: GM1 can time-dependently reduce infarct volume in rats with focal cerebral I/R partly through stabilizing the expression of NMDAR1.展开更多
AIM: To determine the effects of allopurinol, an inhibitor of xanthine oxidase, and apocynin, an inhibitor of NADPH oxidase, on oxidant stress and liver injury caused by hepatic ischemia/reperfusion (I/R) procedure in...AIM: To determine the effects of allopurinol, an inhibitor of xanthine oxidase, and apocynin, an inhibitor of NADPH oxidase, on oxidant stress and liver injury caused by hepatic ischemia/reperfusion (I/R) procedure in mice. METHODS: Mice were pretreated with a xanthine oxidase inhibitor, allopurinol, or NADPH oxidase (NOX) inhibitor, apocynin before the hepatic I/R procedure. Then treated or untreated mice underwent the hepatic I/R procedure. The effects on hepatic injury and superoxide anions were determined after starting reperfusion. RESULTS: A standard warm hepatic I/R procedure led to a marked increase in superoxide anion production as indicated by a superoxide anion tracer, MCLA. At the same time, the procedure caused profound acute liver injury, as indicated by elevated serum alanine aminotransferase and tumor necrosis factor-α levels, reduced liver glutathione levels and elevated malondialdehyde contents, as well as a high apoptotic cell count. All these changes were reversed by the use of apocynin or allopurinol prior to the hepatic I/R procedure. CONCLUSION: Allopurinol and apocynin exerted protective effects on hepatic ischemia/reperfusion injury. The protection is associated with blocking the generationof superoxide anions during the hepatic I/R procedure by inhibiting xanthine oxidase and NADPH oxidase activity.展开更多
In semi arid and arid countries, the increase in production needs sometimes using brackish/saline water for irrigation. In Kairouan and Mahdia (Centre of Tunisia), most of the irrigated areas are by pumping ground w...In semi arid and arid countries, the increase in production needs sometimes using brackish/saline water for irrigation. In Kairouan and Mahdia (Centre of Tunisia), most of the irrigated areas are by pumping ground water from wells and in many cases, water has more than 4 g of salt per liter. To improve farmers' income through using efficiently brackish/saline and rare water, applied research programme was carried out. The methodology adopted was based on selection of six farmers' parcels. Behind water quality and quantity, soil salinity and crop response, the crop cost was studied: initial and final characterization and frequently controlled. Three different water regimes were observed induced three salinity regimes: an exclusively irrigated regime in summer based on using saline water producing continuous accumulation of salts, an irrigated-rained regime in autumn/spring based on alternated saline and fresh water inducing cyclic accumulation and leaching of salts and a rained regime in winter based on fresh water with continuous leaching of salts. At the short term, soil salinity increased under irrigation until equilibrium with the irrigation water quality and decreased by rain which produced an important salts leaching in a very short time. Many tons of salts were added to the initial stock in summer season and most of them are leached to the subsoil under irrigation and by rain in the winter. Salinization affected the deep layer and on the long term, salinization of the aquifer might occur. An important crop yield decrease for the summer crop was obtained but the socio-economic aspect appeared as an important factor conditioning the use of saline water.展开更多
Based on the Pathfinder sea surface temperature(PFSST),the surface axis and its pattern of the Yellow Sea Warm Current(YSWC) are discussed.A structure of double-warm-tongue is found in February and it varies in differ...Based on the Pathfinder sea surface temperature(PFSST),the surface axis and its pattern of the Yellow Sea Warm Current(YSWC) are discussed.A structure of double-warm-tongue is found in February and it varies in different years.Two indexes are calculated to represent the westward shift(WSI) and northward extension(NEI) of the warm water in the Yellow Sea(YS).Wavelet analysis illustrates that the WSI and NEI have prominent periods of 3-6 years and 3-4 years,respectively.The Empirical Orthogonal Function(EOF) decomposition is applied to the winter wind stress curl and the Kuroshio Current(KC) transport,which are believed to play important roles in forcing the variability of the YSWC surface axis.Statistics shows that the WSI is significantly related with the second EOF mode of the wind stress curl in February,which may force the YSWC surface axis moving westward and maintaining the double warm tongues because of its opposite curl in the YSWC domain.The first EOF mode of wind stress curl in January is propitious for inducing the warm tongue in the YS to advance more northward.Hence,the wind stress curls both in January and in February could force variations of the YSWC surface axis;however,the effect of the January wind stress curl is relatively weaker than that of the February.The relationship between the NEI and the KC transport is remarkable,and it seems that the stronger KC supplies more power to push the YSWC northward against the southward wind.展开更多
Since 2000 Chihuahua City has distribution system Waste Water Treatment (WTR), watering city's green areas. The need to cover the growing demand for drinking water has encouraged the use of non-potable water where ...Since 2000 Chihuahua City has distribution system Waste Water Treatment (WTR), watering city's green areas. The need to cover the growing demand for drinking water has encouraged the use of non-potable water where water quality is not an issue despite being used by 10 years ago, it is not known whether nitrogen compounds derived from WTR pose risks to the environment and public health. Therefore, in order to minimize health and environmental risks, this is needed to assess the impact and policy support for water use. The aim of this study is to determine the constitution and the concentration of nitrogen compounds through selection and characterization of park's representative. It's divided into physical properties and main parameters that affect nitrogen transformations. It was determined that sand was material that allowed more favorable oxidation and reduction of nitrate in soil and atmosphere, opposite of the clay, When used WTR, nitrates, nitrites and ammonia nitrogen were identified in the subsurface and NOx and N20 were identified in the atmosphere. When drinking water was used, none of these compounds was found. However, it was determined that despite having high concentrations of nitrates, the WTR was within the limits allowed by the Mexican standard for the use of WTR in public places.展开更多
Objective:To investigate the potential mechanism of electroacupuncture(EA)at bilateral Fengchi(GB20)in treating cerebral ischemia-reperfusion injury and to provide a scientific basis for future experimental research a...Objective:To investigate the potential mechanism of electroacupuncture(EA)at bilateral Fengchi(GB20)in treating cerebral ischemia-reperfusion injury and to provide a scientific basis for future experimental research and clinical applications.Methods:Forty male specific-pathogen-free Sprague-Dawley rats were randomly divided into four groups:a normal group,a normal with EA group,a model group,and a model with EA group,with 10 rats in each group.The normal group received no intervention.The normal with EA group received EA at bilateral Fengchi(GB20).The model group underwent middle cerebral artery occlusion(MCAO)using the suture.The model with EA group underwent MCAO and received EA at bilateral Fengchi(GB20).Cerebral blood flow was monitored using a laser Doppler cerebral blood flow meter.Neurologic damage was assessed using the neurologic deficit score,and motor ability was observed using the CatWalk gait system.The expression of glial fibrillary acidic protein(GFAP)and neuronal nuclei(NeuN)protein,the neuron markers,was detected by Western blotting.The protein expression levels of GFAP and NeuN,as well as the number of positive cells in the motor cortex,were detected using immunofluorescence.Results:Compared to the normal group,the cerebral blood flow values in the model group and the model with EA group decreased by more than 50%during the modeling process(P<0.01)and returned to pre-modeling levels after reperfusion(P>0.05).The neurologic deficit score increased(P<0.05),the average motor velocity decreased(P<0.05),GFAP protein expression and the number of positive cells in the motor cortex increased(P<0.05),and the NeuN protein expression and the number of positive cells decreased(P<0.05)in the model group.Compared to the model group,the neurologic deficit score decreased(P<0.05),the average motor velocity accelerated(P<0.05),GFAP and NeuN protein expression and the number of positive cells in the motor cortex increased(P<0.01)in the model with EA group.Conclusion:EA at bilateral Fengchi(GB20)can reduce neuronal loss and increase GFAP and NeuN protein expression in the motor cortex of rats after ischemia-reperfusion,improve the motor function after ischemic stroke,and accelerate the recovery of balance and stability of the affected limbs.展开更多
Objective: To observe the effects of pretreatment and treatment with electroacupuncture on the ability of learning and memory in rats with ischemia-reperfusion injury, and to provide the research basis for preventing...Objective: To observe the effects of pretreatment and treatment with electroacupuncture on the ability of learning and memory in rats with ischemia-reperfusion injury, and to provide the research basis for preventing and treating the disorder of learning and memory in patients with apoplectic sequelae. Methods: Seventy-two SD rats, clean class, were randomly divided into sham operation group, model group, pretreatment group, and treatment group. The step-through latency and number of errors, nerve injury score, and the number of cholinoceptor in the affected hippocampus were recorded to analyze the effects of pretreatment and treatment with electroacupuncture on the ability of learning and memory in rats with ischemia-reperfusion injury. Results: 1) The rats with ischemia had various signs of nerve injury. Compared with that in the sham operation rats, the nerve injury score in the model rats was lower, the step-through latency and number of errors were increased, and the content of special mAchR was decreased (P〈0.05); 2) Electroacupuncture could decrease the nerve injury score of the model rats. Compared with those in the model group, the step-through latency and number of errors all had significant difference in the pretreatment and treatment groups (P〈0.05); 3) Compared with that in the model group, the content of mAchR was increased in the pretreatment group (P〈0.05), and increased a little in the treatment group. Conclusion: Pretreatment and treatment with electroacupuncture can prolong step-through latency, and展开更多
A sirocco fan using contra-rotating rotors in which an inner rotor is settled inside the sirocco fan rotor and each rotor rotates in an opposite direction was proposed for the purpose of getting the higher pressure an...A sirocco fan using contra-rotating rotors in which an inner rotor is settled inside the sirocco fan rotor and each rotor rotates in an opposite direction was proposed for the purpose of getting the higher pressure and making the structure of a sirocco fan more compact. If the high discharge pressure is obtained with the adoption of the contra-rotating rotors, it could be used for various purposes. Pressure coefficient of a sirocco fan with contra-rotating rotors is 2.5 times as high as the conventional sirocco fan and the maximum efficiency point of contra-rotating rotors shifts to larger flow rate than a conventional sirocco fan. On the other hand, it was clarified from the flow measurement results that circumferential velocity component at the outlet of the outer rotor of contra-rotating ro- tors becomes larger than a conventional one. In the present paper, the performance of a conventional sirocco fan and a sirocco fan with contra-rotating rotors are shown and the internal flow field at the outlet of outer rotor of both cases is clarified. Then, the effect of different kind of contra-rotating rotors on the performance and internal flow field is investigated and the rotor design with higher performance would be discussed.展开更多
基金Project (No. 2004QN012) supported by the Youth Talent SpecialFund of Health Bureau of Zhejiang Province the National BasicResearch Program (973) of China (No. G1999054000) and the Na-tional Natural Science Foundation of China (No. 30371637)
文摘Objective: To determine the protective effect of monosialoganglionside (GM1) and evaluate the influence of GM1 on expression of N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in Sprague-Dawley (SD) rats with focal cerebral ische- mia-reperfusion (I/R). Methods: Left middle cerebral artery (MCA) was occluded by an intraluminal suture for 1 h and the brain was reperfused for 72 h in SD rats when infarct volume was measured, GM1 (10 mg/kg) was given ip (intraperitoneally) at 5 min (group A), 1 h (group B) and 2 h (group C) after MCA occlusion (MCAo). Expression of NMDAR1 was detected by Western blot at various time after reperfusion (4 h, 6 h, 24 h, 48 h and 72 h) in ischemic hemispheres of the rats with or without GM1 admin- istered. Results: (1) Adjusted relative infarct volumes of groups A and B were significantly smaller than that of group C and the control group (P<0.01 and P<0.05, respectively). (2) Expression level of NMDAR1 was temporally high at 6 h after reperfusion, and dipped below the normal level at 72 h after reperfusion. GM1 at 5 min after MCAo significantly suppressed the expression of NMDAR1 at 6 h after reperfusion (P<0.05 vs the control). At 72 h after reperfusion, the NMDAR1 expression level of rats treated with GM1 administered (at 5 min or 2 h after MCAo) was significantly higher than that of the control (P<0.05). Conclusion: GM1 can time-dependently reduce infarct volume in rats with focal cerebral I/R partly through stabilizing the expression of NMDAR1.
基金The National Institute of Diabetes, Digestive and Kidney Diseases (NIDDK), DK069939 the Technology Transfer Grant (TTG) by UC Davis Medical Center to Wu J
文摘AIM: To determine the effects of allopurinol, an inhibitor of xanthine oxidase, and apocynin, an inhibitor of NADPH oxidase, on oxidant stress and liver injury caused by hepatic ischemia/reperfusion (I/R) procedure in mice. METHODS: Mice were pretreated with a xanthine oxidase inhibitor, allopurinol, or NADPH oxidase (NOX) inhibitor, apocynin before the hepatic I/R procedure. Then treated or untreated mice underwent the hepatic I/R procedure. The effects on hepatic injury and superoxide anions were determined after starting reperfusion. RESULTS: A standard warm hepatic I/R procedure led to a marked increase in superoxide anion production as indicated by a superoxide anion tracer, MCLA. At the same time, the procedure caused profound acute liver injury, as indicated by elevated serum alanine aminotransferase and tumor necrosis factor-α levels, reduced liver glutathione levels and elevated malondialdehyde contents, as well as a high apoptotic cell count. All these changes were reversed by the use of apocynin or allopurinol prior to the hepatic I/R procedure. CONCLUSION: Allopurinol and apocynin exerted protective effects on hepatic ischemia/reperfusion injury. The protection is associated with blocking the generationof superoxide anions during the hepatic I/R procedure by inhibiting xanthine oxidase and NADPH oxidase activity.
文摘In semi arid and arid countries, the increase in production needs sometimes using brackish/saline water for irrigation. In Kairouan and Mahdia (Centre of Tunisia), most of the irrigated areas are by pumping ground water from wells and in many cases, water has more than 4 g of salt per liter. To improve farmers' income through using efficiently brackish/saline and rare water, applied research programme was carried out. The methodology adopted was based on selection of six farmers' parcels. Behind water quality and quantity, soil salinity and crop response, the crop cost was studied: initial and final characterization and frequently controlled. Three different water regimes were observed induced three salinity regimes: an exclusively irrigated regime in summer based on using saline water producing continuous accumulation of salts, an irrigated-rained regime in autumn/spring based on alternated saline and fresh water inducing cyclic accumulation and leaching of salts and a rained regime in winter based on fresh water with continuous leaching of salts. At the short term, soil salinity increased under irrigation until equilibrium with the irrigation water quality and decreased by rain which produced an important salts leaching in a very short time. Many tons of salts were added to the initial stock in summer season and most of them are leached to the subsoil under irrigation and by rain in the winter. Salinization affected the deep layer and on the long term, salinization of the aquifer might occur. An important crop yield decrease for the summer crop was obtained but the socio-economic aspect appeared as an important factor conditioning the use of saline water.
基金Supported by the National Basic Research Program of China (973 Program) (No 2005C B422308)the National High-tech Research and Development Program (863 Program) (No 2006AA09Z149)the China International Science and Technology Cooperation Program (No2006DFB21250)
文摘Based on the Pathfinder sea surface temperature(PFSST),the surface axis and its pattern of the Yellow Sea Warm Current(YSWC) are discussed.A structure of double-warm-tongue is found in February and it varies in different years.Two indexes are calculated to represent the westward shift(WSI) and northward extension(NEI) of the warm water in the Yellow Sea(YS).Wavelet analysis illustrates that the WSI and NEI have prominent periods of 3-6 years and 3-4 years,respectively.The Empirical Orthogonal Function(EOF) decomposition is applied to the winter wind stress curl and the Kuroshio Current(KC) transport,which are believed to play important roles in forcing the variability of the YSWC surface axis.Statistics shows that the WSI is significantly related with the second EOF mode of the wind stress curl in February,which may force the YSWC surface axis moving westward and maintaining the double warm tongues because of its opposite curl in the YSWC domain.The first EOF mode of wind stress curl in January is propitious for inducing the warm tongue in the YS to advance more northward.Hence,the wind stress curls both in January and in February could force variations of the YSWC surface axis;however,the effect of the January wind stress curl is relatively weaker than that of the February.The relationship between the NEI and the KC transport is remarkable,and it seems that the stronger KC supplies more power to push the YSWC northward against the southward wind.
文摘Since 2000 Chihuahua City has distribution system Waste Water Treatment (WTR), watering city's green areas. The need to cover the growing demand for drinking water has encouraged the use of non-potable water where water quality is not an issue despite being used by 10 years ago, it is not known whether nitrogen compounds derived from WTR pose risks to the environment and public health. Therefore, in order to minimize health and environmental risks, this is needed to assess the impact and policy support for water use. The aim of this study is to determine the constitution and the concentration of nitrogen compounds through selection and characterization of park's representative. It's divided into physical properties and main parameters that affect nitrogen transformations. It was determined that sand was material that allowed more favorable oxidation and reduction of nitrate in soil and atmosphere, opposite of the clay, When used WTR, nitrates, nitrites and ammonia nitrogen were identified in the subsurface and NOx and N20 were identified in the atmosphere. When drinking water was used, none of these compounds was found. However, it was determined that despite having high concentrations of nitrates, the WTR was within the limits allowed by the Mexican standard for the use of WTR in public places.
文摘Objective:To investigate the potential mechanism of electroacupuncture(EA)at bilateral Fengchi(GB20)in treating cerebral ischemia-reperfusion injury and to provide a scientific basis for future experimental research and clinical applications.Methods:Forty male specific-pathogen-free Sprague-Dawley rats were randomly divided into four groups:a normal group,a normal with EA group,a model group,and a model with EA group,with 10 rats in each group.The normal group received no intervention.The normal with EA group received EA at bilateral Fengchi(GB20).The model group underwent middle cerebral artery occlusion(MCAO)using the suture.The model with EA group underwent MCAO and received EA at bilateral Fengchi(GB20).Cerebral blood flow was monitored using a laser Doppler cerebral blood flow meter.Neurologic damage was assessed using the neurologic deficit score,and motor ability was observed using the CatWalk gait system.The expression of glial fibrillary acidic protein(GFAP)and neuronal nuclei(NeuN)protein,the neuron markers,was detected by Western blotting.The protein expression levels of GFAP and NeuN,as well as the number of positive cells in the motor cortex,were detected using immunofluorescence.Results:Compared to the normal group,the cerebral blood flow values in the model group and the model with EA group decreased by more than 50%during the modeling process(P<0.01)and returned to pre-modeling levels after reperfusion(P>0.05).The neurologic deficit score increased(P<0.05),the average motor velocity decreased(P<0.05),GFAP protein expression and the number of positive cells in the motor cortex increased(P<0.05),and the NeuN protein expression and the number of positive cells decreased(P<0.05)in the model group.Compared to the model group,the neurologic deficit score decreased(P<0.05),the average motor velocity accelerated(P<0.05),GFAP and NeuN protein expression and the number of positive cells in the motor cortex increased(P<0.01)in the model with EA group.Conclusion:EA at bilateral Fengchi(GB20)can reduce neuronal loss and increase GFAP and NeuN protein expression in the motor cortex of rats after ischemia-reperfusion,improve the motor function after ischemic stroke,and accelerate the recovery of balance and stability of the affected limbs.
基金The Key laboratory of acupuncture-immune effects of State Administration of Traditional Chinese MedicineShanghai Leading Academic Discipline Project (S30304)+1 种基金the Special Item of Basic Theory of Traditional Chinese Medicine, National Basic Research Program of China (973 Program, 2005CB523306)the Scientific and Technologic Fund of Shanghai (014319364)
文摘Objective: To observe the effects of pretreatment and treatment with electroacupuncture on the ability of learning and memory in rats with ischemia-reperfusion injury, and to provide the research basis for preventing and treating the disorder of learning and memory in patients with apoplectic sequelae. Methods: Seventy-two SD rats, clean class, were randomly divided into sham operation group, model group, pretreatment group, and treatment group. The step-through latency and number of errors, nerve injury score, and the number of cholinoceptor in the affected hippocampus were recorded to analyze the effects of pretreatment and treatment with electroacupuncture on the ability of learning and memory in rats with ischemia-reperfusion injury. Results: 1) The rats with ischemia had various signs of nerve injury. Compared with that in the sham operation rats, the nerve injury score in the model rats was lower, the step-through latency and number of errors were increased, and the content of special mAchR was decreased (P〈0.05); 2) Electroacupuncture could decrease the nerve injury score of the model rats. Compared with those in the model group, the step-through latency and number of errors all had significant difference in the pretreatment and treatment groups (P〈0.05); 3) Compared with that in the model group, the content of mAchR was increased in the pretreatment group (P〈0.05), and increased a little in the treatment group. Conclusion: Pretreatment and treatment with electroacupuncture can prolong step-through latency, and
文摘A sirocco fan using contra-rotating rotors in which an inner rotor is settled inside the sirocco fan rotor and each rotor rotates in an opposite direction was proposed for the purpose of getting the higher pressure and making the structure of a sirocco fan more compact. If the high discharge pressure is obtained with the adoption of the contra-rotating rotors, it could be used for various purposes. Pressure coefficient of a sirocco fan with contra-rotating rotors is 2.5 times as high as the conventional sirocco fan and the maximum efficiency point of contra-rotating rotors shifts to larger flow rate than a conventional sirocco fan. On the other hand, it was clarified from the flow measurement results that circumferential velocity component at the outlet of the outer rotor of contra-rotating ro- tors becomes larger than a conventional one. In the present paper, the performance of a conventional sirocco fan and a sirocco fan with contra-rotating rotors are shown and the internal flow field at the outlet of outer rotor of both cases is clarified. Then, the effect of different kind of contra-rotating rotors on the performance and internal flow field is investigated and the rotor design with higher performance would be discussed.