This study aimed to achieve rapid detection of Pseudomonas syringae pv.tabaci,the pathogen of tobacco wildfire disease.The specific primers and probes for recombinase-aided amplification(RAA)were designed with HrpZ as...This study aimed to achieve rapid detection of Pseudomonas syringae pv.tabaci,the pathogen of tobacco wildfire disease.The specific primers and probes for recombinase-aided amplification(RAA)were designed with HrpZ as the target gene.RAA was then combined with the lateral flow dipstick(LFD)to establish a LFD-RAA-based rapid detection system for the pathogen.Furthermore,the detection performance of the established method was tested.The results showed that the LFDRAA method had high specificity.The amplification could be completed after 25 min of reaction at 39℃.The sensitivity of the established method reached 0.0001 ng/μL,which was superior to that of PCR detection.Moreover,the LFD-RAA method could quickly detect P.syringae pv.tabaci from tobacco leaves,demonstrating field applicability.To sum up,the LFD-RAA method established in this study can be applied in the rapid detection and early diagnosis of tobacco wildfire disease.展开更多
In high-rise building fires, the most immediate threat to passenger life and safety evacuation is the smoke inhalation. Some traditional models for smoke prevention and exhaust are analyzed and compared with the smoke...In high-rise building fires, the most immediate threat to passenger life and safety evacuation is the smoke inhalation. Some traditional models for smoke prevention and exhaust are analyzed and compared with the smoke-preventing air curtain. The rationality and the feasibility of the air curtain are theoretically expounded. The air volume, tuyere width and jet velocity in the air curtain experiment are designed according to the theoreti- cal calculation model. Experimental results indicate that the effect of air curtain to prevent smoke diffusion is re- markable as the volume ratio of air-smoke is about 0. 6, the jet angle is between 25^o and 35^o, and the jet thickness is between 25 mm and 45 mm. The efficiency of air curtain can reach 98% on the entraining effect. Meanwhile, experiments verify the theorectical calculation.展开更多
文摘This study aimed to achieve rapid detection of Pseudomonas syringae pv.tabaci,the pathogen of tobacco wildfire disease.The specific primers and probes for recombinase-aided amplification(RAA)were designed with HrpZ as the target gene.RAA was then combined with the lateral flow dipstick(LFD)to establish a LFD-RAA-based rapid detection system for the pathogen.Furthermore,the detection performance of the established method was tested.The results showed that the LFDRAA method had high specificity.The amplification could be completed after 25 min of reaction at 39℃.The sensitivity of the established method reached 0.0001 ng/μL,which was superior to that of PCR detection.Moreover,the LFD-RAA method could quickly detect P.syringae pv.tabaci from tobacco leaves,demonstrating field applicability.To sum up,the LFD-RAA method established in this study can be applied in the rapid detection and early diagnosis of tobacco wildfire disease.
文摘In high-rise building fires, the most immediate threat to passenger life and safety evacuation is the smoke inhalation. Some traditional models for smoke prevention and exhaust are analyzed and compared with the smoke-preventing air curtain. The rationality and the feasibility of the air curtain are theoretically expounded. The air volume, tuyere width and jet velocity in the air curtain experiment are designed according to the theoreti- cal calculation model. Experimental results indicate that the effect of air curtain to prevent smoke diffusion is re- markable as the volume ratio of air-smoke is about 0. 6, the jet angle is between 25^o and 35^o, and the jet thickness is between 25 mm and 45 mm. The efficiency of air curtain can reach 98% on the entraining effect. Meanwhile, experiments verify the theorectical calculation.
