This paper looks into David Herbert Lawrence＇s characterization of Mrs. Morel in his famous novel Sons and Lovers （1962）. Mrs. Morel has long been regarded by feminist critics as the destroyed, a victim destroyed b...This paper looks into David Herbert Lawrence＇s characterization of Mrs. Morel in his famous novel Sons and Lovers （1962）. Mrs. Morel has long been regarded by feminist critics as the destroyed, a victim destroyed by the male-dominated society, and her son Paul has been viewed as a destroyer of women. Based on a close reading of the novel, this paper examines Lawrence＇s characterization of Mrs. Morel and reveals that Mrs Morel actually belongs to the destroyer instead of the destroyed through an analysis of her relationship with the three male characters in the novel, thus providing new insights into the understanding of this classic novel.展开更多
Royal jelly （R J） is a well-known bioactive substance. It contains large amounts of major royal jelly proteins （MRJPs）, which express growth-factor-like activity in several animal and human cell lines. However, th...Royal jelly （R J） is a well-known bioactive substance. It contains large amounts of major royal jelly proteins （MRJPs）, which express growth-factor-like activity in several animal and human cell lines. However, the question on whether MRJPs possess growth-factor-like activity on all types of cell cultures remains. In order to determine whether MRJPs can be used as an alternative to fetal bovine serum （FBS） in different types of human cell culture, the prolif- eration of the complex serum with different ratios of MRJPs/FBS （M/F） was evaluated on five cell lines： 293T, HFL-I, 231, HCT116, and Changliver using MTT （3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide） assay. The proliferation activity of the combination of the complex M/F serum with cytokines on the test cell lines was also measured. The results demonstrated that the complex serum with M/F 6/4 possessed the highest proliferation activity similar to or in excess of FBS. However, no activity of complex medium with M/F 6/4 was observed in 231 cells, indicating a selectivity of MRJPs on cell types. Compared with the complex medium with M/F 6/4, the complex medium with M/F 6/4 together with two cytokines, epidermal growth factor （EGF） and insulin-transferrin-selenium （ITS）, pro- moted proliferations of Changliver, 293T, HCT116, and H FL-I by 18.73%-56.19% （P〈0.01）. Our findings demonstrate that MRJPs could partially replace FBS in culturing many human cell lines.展开更多
文摘This paper looks into David Herbert Lawrence＇s characterization of Mrs. Morel in his famous novel Sons and Lovers （1962）. Mrs. Morel has long been regarded by feminist critics as the destroyed, a victim destroyed by the male-dominated society, and her son Paul has been viewed as a destroyer of women. Based on a close reading of the novel, this paper examines Lawrence＇s characterization of Mrs. Morel and reveals that Mrs Morel actually belongs to the destroyer instead of the destroyed through an analysis of her relationship with the three male characters in the novel, thus providing new insights into the understanding of this classic novel.
基金Project supported by the National Natural Science Foundation of China(No.31271848)the Important Scientific&Technical Innovation Project of Hangzhou(No.20131812A25)the Foundation of Fuli Institute of Food Science of Zhejiang University(No.KY201404),China
文摘Royal jelly （R J） is a well-known bioactive substance. It contains large amounts of major royal jelly proteins （MRJPs）, which express growth-factor-like activity in several animal and human cell lines. However, the question on whether MRJPs possess growth-factor-like activity on all types of cell cultures remains. In order to determine whether MRJPs can be used as an alternative to fetal bovine serum （FBS） in different types of human cell culture, the prolif- eration of the complex serum with different ratios of MRJPs/FBS （M/F） was evaluated on five cell lines： 293T, HFL-I, 231, HCT116, and Changliver using MTT （3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide） assay. The proliferation activity of the combination of the complex M/F serum with cytokines on the test cell lines was also measured. The results demonstrated that the complex serum with M/F 6/4 possessed the highest proliferation activity similar to or in excess of FBS. However, no activity of complex medium with M/F 6/4 was observed in 231 cells, indicating a selectivity of MRJPs on cell types. Compared with the complex medium with M/F 6/4, the complex medium with M/F 6/4 together with two cytokines, epidermal growth factor （EGF） and insulin-transferrin-selenium （ITS）, pro- moted proliferations of Changliver, 293T, HCT116, and H FL-I by 18.73%-56.19% （P〈0.01）. Our findings demonstrate that MRJPs could partially replace FBS in culturing many human cell lines.
