The Chinese tree shrew (Tupaia belangeri chinensis) is a small experimental animal with a close affinity to primates. This species has long been proposed to be an alternative experimental animal to primates in biomedi...The Chinese tree shrew (Tupaia belangeri chinensis) is a small experimental animal with a close affinity to primates. This species has long been proposed to be an alternative experimental animal to primates in biomedical research. Despite decades of study, there is no pure breed for this animal, and the overall genetic diversity of wild tree shrews remains largely unknown. In order to obtain a set of genetic markers for evaluating the genetic diversity of tree shrew wild populations and tracing the lineages in inbreeding populations, we developed 12 polymorphic microsatellite markers from the genomic DNA of the tree shrew. An analysis of a wild population of 117 individuals collected from the suburb of Kunming, China, showed that these loci exhibited a highly expected heterozygosity (0.616). These 12 microsatellites were sufficient for individual identification and parentage analysis. The microsatellite markers developed in this study will be of use in evaluating genetic diversity and lineage tracing for the tree shrew.展开更多
This paper attempts to analyze Paul Morel＇s instinctual drives--in D. H. Lawrence＇s Sons and Lovers （2006}---toward survival, neurotic refusal to accept life, and his will to give life meaning after a fierce and pr...This paper attempts to analyze Paul Morel＇s instinctual drives--in D. H. Lawrence＇s Sons and Lovers （2006}---toward survival, neurotic refusal to accept life, and his will to give life meaning after a fierce and prolonged internal strife--in the light of psychoanalysis. It will explore how his ＂Id＂ strives to bring about the satisfaction of instinctual needs on the basis of the pleasure principle driving him to seek pleasure. His ＂libido＂--psychic energy, emanating from the id, especially the sexual urge--has empowered his ＂Eros＂ or life instinct. It will also explore how ＂Thanatos＂ or death instinct, in contrast, has yearned for an almost lifeless state and curbed entering into human relationships as experienced in his last rejection of Miriam after his mother＇s death leaving a great trauma on Paul who has been on the verse of extinction as a result. Therefore, Eros and Thanatos, both coincide and conflict within him, are dynamically involved and thus are interacted infusing life not stagnation in Paulin consequence展开更多
基金supported by the National 863 Project of China (2012AA021801)grants from Chinese Academy of Sciences (KSCX2-EW-R-11 and KSCX2-EW-J-23)Yunnan Province (2009CI119)
文摘The Chinese tree shrew (Tupaia belangeri chinensis) is a small experimental animal with a close affinity to primates. This species has long been proposed to be an alternative experimental animal to primates in biomedical research. Despite decades of study, there is no pure breed for this animal, and the overall genetic diversity of wild tree shrews remains largely unknown. In order to obtain a set of genetic markers for evaluating the genetic diversity of tree shrew wild populations and tracing the lineages in inbreeding populations, we developed 12 polymorphic microsatellite markers from the genomic DNA of the tree shrew. An analysis of a wild population of 117 individuals collected from the suburb of Kunming, China, showed that these loci exhibited a highly expected heterozygosity (0.616). These 12 microsatellites were sufficient for individual identification and parentage analysis. The microsatellite markers developed in this study will be of use in evaluating genetic diversity and lineage tracing for the tree shrew.
文摘This paper attempts to analyze Paul Morel＇s instinctual drives--in D. H. Lawrence＇s Sons and Lovers （2006}---toward survival, neurotic refusal to accept life, and his will to give life meaning after a fierce and prolonged internal strife--in the light of psychoanalysis. It will explore how his ＂Id＂ strives to bring about the satisfaction of instinctual needs on the basis of the pleasure principle driving him to seek pleasure. His ＂libido＂--psychic energy, emanating from the id, especially the sexual urge--has empowered his ＂Eros＂ or life instinct. It will also explore how ＂Thanatos＂ or death instinct, in contrast, has yearned for an almost lifeless state and curbed entering into human relationships as experienced in his last rejection of Miriam after his mother＇s death leaving a great trauma on Paul who has been on the verse of extinction as a result. Therefore, Eros and Thanatos, both coincide and conflict within him, are dynamically involved and thus are interacted infusing life not stagnation in Paulin consequence
