A selective and sensitive HPLC method has been developed and validated for the quantification of ganoderic acid T in rat plasma. A reverse-phase column was used with UV detection at 245 nm. The mobile phase consisted ...A selective and sensitive HPLC method has been developed and validated for the quantification of ganoderic acid T in rat plasma. A reverse-phase column was used with UV detection at 245 nm. The mobile phase consisted of methanol-water-acetic acid （95.5：4.5：0.5, v/v/v） run at a flow rate of 1 mL/min. Testosterone propionate was used as the internal standard. The standard curve was linear over the range of 0.05-50 ~tg/mL in rat plasma with a linear correlation coefficient greater than 0.999. The lower limit of quantification of this assay was 20 ng/mL. The recoveries of samples at 0.05, 2 and 40 μg/mL were 97.6%, 98.4% and 103.2%, respectively. The relative standard deviations of intra- and inter-day assay variations were less than 8.2%. The usefulness of the assay was confirmed by the successful analysis of plasma samples from a pharmacokinetics study in rats. Following a single dose of ganoderic acid T （5 mg/kg for i.v. or 14 mg/kg for p.o.）, the main pharmacokinetic parameters by intravenous injection were： t1/2α （5.46±1.25） min; t1/2β：（227.18±11.40） min; CL： （1.09±0.16） mL/（kg·min）; A UC0-12 h： （3939.13±311.14） μg.min/mL; AUC0-12h （4681.96±710.70）μg.min/mL and the absolute bioavailability was 41.98%±2.40%. This method is simple, sensitive, and accurate. It is suitable for pharmacokinetic studies of ganoderic acid in rats.展开更多
基金Program for Shanghai Technology Innovation Action Plan,the Modernization of Chinese Medicine Special Project (Grant No.08DZ1971900)111 Program of China(Grant No. B07023)
文摘A selective and sensitive HPLC method has been developed and validated for the quantification of ganoderic acid T in rat plasma. A reverse-phase column was used with UV detection at 245 nm. The mobile phase consisted of methanol-water-acetic acid （95.5：4.5：0.5, v/v/v） run at a flow rate of 1 mL/min. Testosterone propionate was used as the internal standard. The standard curve was linear over the range of 0.05-50 ~tg/mL in rat plasma with a linear correlation coefficient greater than 0.999. The lower limit of quantification of this assay was 20 ng/mL. The recoveries of samples at 0.05, 2 and 40 μg/mL were 97.6%, 98.4% and 103.2%, respectively. The relative standard deviations of intra- and inter-day assay variations were less than 8.2%. The usefulness of the assay was confirmed by the successful analysis of plasma samples from a pharmacokinetics study in rats. Following a single dose of ganoderic acid T （5 mg/kg for i.v. or 14 mg/kg for p.o.）, the main pharmacokinetic parameters by intravenous injection were： t1/2α （5.46±1.25） min; t1/2β：（227.18±11.40） min; CL： （1.09±0.16） mL/（kg·min）; A UC0-12 h： （3939.13±311.14） μg.min/mL; AUC0-12h （4681.96±710.70）μg.min/mL and the absolute bioavailability was 41.98%±2.40%. This method is simple, sensitive, and accurate. It is suitable for pharmacokinetic studies of ganoderic acid in rats.
