Objective To elucidate the important functions of microRNAs （miRNAs） in regulating synaptic assembly and function, we performed a computational analysis for the genetic loci required for the synaptic structure and f...Objective To elucidate the important functions of microRNAs （miRNAs） in regulating synaptic assembly and function, we performed a computational analysis for the genetic loci required for the synaptic structure and function and their corresponding miRNAs in C. elegans. Methods Total 198 genetic loci required for the synaptic structure and function were selected. Sequence alignment was combined with E value evaluation to investigate and identify the possible corresponding miRNAs. Results Total 163 genes among the 198 genetic loci selected have their possibly corresponding regulatory miRNA （s）, which covered most of the important genetic loci required for the synaptic structure and function. Moreover, only 22 genes among the analyzed 38 genetic loci encoding synaptic proteins have more possibility to under the control of non-coding RNA genes. In addition, the distribution of miRNAs along the 3＇ untranslated region （UTR） of these 22 genes exhibits different patterns. Condusion Here we provide the computational screen and analysis results for the genetic loci required for synaptic structure and function and their possible corresponding miRNAs. These data will be useful for the further attempt to systematically determine the roles of miRNAs in synaptic assembly and function regulation in worms.展开更多
The phenotypic traits of high prolificacy, low fat on the rib eye muscle and high tolerance to internal parasites make the Barbados Blackbelly （BBB） sheep a valuable economic resource for small subsistence farmers o...The phenotypic traits of high prolificacy, low fat on the rib eye muscle and high tolerance to internal parasites make the Barbados Blackbelly （BBB） sheep a valuable economic resource for small subsistence farmers on some Caribbean islands. However, the determination of what is a purebred BBB sheep has long been a contentious issue because of the subjectivity. It is therefore necessary to have a more reliable means of identifying what is a purebred BBB sheep for purposes of conservation and breed improvement. Three geographically isolated populations of BBB sheep were genetically analysed at 19 microsatellite loci. Eighteen of the microsatellites were found to be useful for genetic analysis of the BBB sheep, based on polymorphic information content （PIC） values （0.5 〈 PIC 〉 0.25）. One microsatellite was not useful for genetic analysis based on PIC （0.111）, however it is considered to be a locus of interest because it is fully inbred （f = 1） and homozygous in all of the populations analysed. The analysis also showed that the purebred population of BBB sheep can be distinguished from other populations by formation of distinct clusters when subjected to analysis of molecular variance （AMOVA） and the construction of a rooted consensus tree using the Neighbour-Joining method and pair-wise distances based on marker estimated kinships.展开更多
Node positioning is a fundamental problem in applications of wireless sensor networks (WSNs). In this paper, a new range-free algorithm, called spring swarm localization algorithm (SSLA), is proposed for positioning W...Node positioning is a fundamental problem in applications of wireless sensor networks (WSNs). In this paper, a new range-free algorithm, called spring swarm localization algorithm (SSLA), is proposed for positioning WSNs. To determine the locations of sensor nodes, the proposed algorithm uses network topology information and a small fraction of sensor nodes which know their locations. Numerical simulations show that high positioning accuracy can be obtained by using the algorithm. Some examples are given to illustrate the effectiveness of the algorithm.展开更多
文摘Objective To elucidate the important functions of microRNAs （miRNAs） in regulating synaptic assembly and function, we performed a computational analysis for the genetic loci required for the synaptic structure and function and their corresponding miRNAs in C. elegans. Methods Total 198 genetic loci required for the synaptic structure and function were selected. Sequence alignment was combined with E value evaluation to investigate and identify the possible corresponding miRNAs. Results Total 163 genes among the 198 genetic loci selected have their possibly corresponding regulatory miRNA （s）, which covered most of the important genetic loci required for the synaptic structure and function. Moreover, only 22 genes among the analyzed 38 genetic loci encoding synaptic proteins have more possibility to under the control of non-coding RNA genes. In addition, the distribution of miRNAs along the 3＇ untranslated region （UTR） of these 22 genes exhibits different patterns. Condusion Here we provide the computational screen and analysis results for the genetic loci required for synaptic structure and function and their possible corresponding miRNAs. These data will be useful for the further attempt to systematically determine the roles of miRNAs in synaptic assembly and function regulation in worms.
文摘The phenotypic traits of high prolificacy, low fat on the rib eye muscle and high tolerance to internal parasites make the Barbados Blackbelly （BBB） sheep a valuable economic resource for small subsistence farmers on some Caribbean islands. However, the determination of what is a purebred BBB sheep has long been a contentious issue because of the subjectivity. It is therefore necessary to have a more reliable means of identifying what is a purebred BBB sheep for purposes of conservation and breed improvement. Three geographically isolated populations of BBB sheep were genetically analysed at 19 microsatellite loci. Eighteen of the microsatellites were found to be useful for genetic analysis of the BBB sheep, based on polymorphic information content （PIC） values （0.5 〈 PIC 〉 0.25）. One microsatellite was not useful for genetic analysis based on PIC （0.111）, however it is considered to be a locus of interest because it is fully inbred （f = 1） and homozygous in all of the populations analysed. The analysis also showed that the purebred population of BBB sheep can be distinguished from other populations by formation of distinct clusters when subjected to analysis of molecular variance （AMOVA） and the construction of a rooted consensus tree using the Neighbour-Joining method and pair-wise distances based on marker estimated kinships.
基金supported by the National Natural Science Foundation of China (Grant Nos. 10832006 and 60872093)
文摘Node positioning is a fundamental problem in applications of wireless sensor networks (WSNs). In this paper, a new range-free algorithm, called spring swarm localization algorithm (SSLA), is proposed for positioning WSNs. To determine the locations of sensor nodes, the proposed algorithm uses network topology information and a small fraction of sensor nodes which know their locations. Numerical simulations show that high positioning accuracy can be obtained by using the algorithm. Some examples are given to illustrate the effectiveness of the algorithm.
