辽河铁岭段水质监测点位布设及分析

对辽河铁岭段水质监测点位布设及各点位在环境监测、研究污染控制、环境管理和制定环境规划等方面的影响及作用进行了分析。

基于“数据可视化”对吉林省××市APP简易事故点位及与死亡事故点位关联的深度分析

数据"可视化"是关于数据"视觉"表现形式的科技研究,主要旨在借助"图形化"手段,清晰有效地传达与沟通信息。我们通过"吉林交警简易事故APP"和"吉林省交通事故网上办案监督系统",利用交通地理信息系统(T-GIS)及数据可视化技术对简易事故和死亡事故的聚类范围进行圈定,直接标划事故多发点段,从点位、统计、关联等三个方面进行深度分析。2017年吉林省全面启动"吉林交警APP",截止2018年4月,全省在线快处快赔简易事故突破11.5万起。为了积极探索情指一体化工作机制,坚持"以信息主导警务",我们对吉林省××市APP简易事故点位进行深度分析,找准预防事故的最大下降点,为秩序管理工作提供点对点的、强有力的科学依据。

边角后方交会计算方法及精度分析

文章论述了野外测量中边角后方交会的一种方法,并进行了精度评定和实际应用举例。该方法简捷易行,克服了已知点不通视给测量工作带来的麻烦,在实际工作中具有较好的应用价值。

GPS-RTK实时动态定位结合支导线在地形测量中的应用

为了解决在GPS信号传输严重受阻、RTK接收机失锁严重的地区进行地形测量时影响正常测量精度的问题,结合某市融江西岸整治综合项目,使用GPS-RTK技术结合全站仪支导线法布设图根点的方法,对测量的点位精度进行分析,探究精度是否满足大比例尺地形图测量的精度要求。结果表明:该方法灵活实用,降低了外业劳动强度,极大提高了测量效率。尤其GPS-RTK布设的图根点没有累计误差的存在,提高了测量的精度,对信号受干扰地区进行大比例地形测量具有借鉴意义。

Modeling GPS multipath effect based on spherical cap harmonic analysis

Most GPS positioning errors can be eliminated or removed by the differential technique or the modeling method,but the multipath effect is a special kind of system or gross error,so it is difficult to be simulated or eliminated.In order to improve the accuracy of GPS positioning,the single-epoch pseudorange multipath effects at GPS station were calculated,and firstly modeled based on the spherical cap harmonic（SCH）,which is the function of satellite longitude and latitude with the robust method.The accuracy of the kinematic point positioning technique was improved by correcting pseudorange observations with the multipath effect calculated by the SCH model,especially in the elevation direction.The spherical cap harmonic can be used to model the pseudorange multipath effect.

SSR Cluster and Fertility Loci Analysis of GC13

[Objective] The research aimed to clarify the genetic mechanism of special wide compatibility of GC13.[Method] The clustering analyses of GC13,five indica,five japonica and five wide compatibility varieties were carried out by using 70 SSR primers.[Result] GC13 was clustered into japonica group and had far genetic relationship with indica and wide compatibility variety.Two fertility loci were detected in GC13,in which one closely linked to RM225 on chromosome 6.According to the position on the chromosome,it speculated that this locus was allelic to S5.GC13 carried the allelic gene S5-n at this locus.The other locus closely linked to RM408 on chromosome 8 and was provisionally designated as Sg(t).At this locus,GC13 carried Sg(t)-i allelic gene,which was consistent with IR36.The effect of S5 locus was stronger than that of Sg(t).[Conclusion] The research laid the good foundation for using the wide compatibility line GC13 to breed the hybrid between subspecies.

Characterization of a S-locus-related Receptor-like Kinase Cluster in Rice Chromosome 4

We have identified 14 S _locus glycoprotein (SLG)_related protein kinase genes in a 323 kb contig of rice (Oryza sativa L.) chromosome 4 and we detected the transcription pattern of this gene cluster by reverse transcription_polymerase reaction (RT_PCR). RT_PCR results revealed that nine putative genes were transcribed in rice and these genes had the different expression patterns: two genes are expressed predominantly in reproductive tissues while the other seven genes are expressed in both reproductive and vegetative tissues. Analysis of the predicted amino acid sequences demonstrated that the extracellular receptor domains are highly homologous to SLG of Brassica, whereas the cytoplasmic kinase domains contain conserved amino acids present in serine/threonine kinases.

Design and performance analysis of wireless sensor network location node system for underground mine

Aiming at the application of a wireless sensor network to locating miners in underground mine,we design a wireless sensor network location node system,considering the communication performance and the intrinsic safety. The location node system consists of a mobile node,several fixed nodes,and a sink node,all of whose circuits were designed based on CC2430. A varistor and a RC circuit were used in the reset circuit of a sensor node to guarantee the intrinsic safety by reducing discharge energy,the theoretical analysis of the discharge energy shows that the reset circuit is an intrinsic safety one. The analysis and simulation about the performance of the location node system are discussed,such as network communication delay and packet loss rate,the results show that the highest network communication delay of the system is about 0.11 seconds,and the highest packet loss rate is about 0.13,which assures the location node system has a high reliability,and can locate miners in the underground mine.

Computational analysis of genetic loci required for synapse structure and function and their corresponding microRNAs in C. elegans

Objective To elucidate the important functions of microRNAs （miRNAs） in regulating synaptic assembly and function, we performed a computational analysis for the genetic loci required for the synaptic structure and function and their corresponding miRNAs in C. elegans. Methods Total 198 genetic loci required for the synaptic structure and function were selected. Sequence alignment was combined with E value evaluation to investigate and identify the possible corresponding miRNAs. Results Total 163 genes among the 198 genetic loci selected have their possibly corresponding regulatory miRNA （s）, which covered most of the important genetic loci required for the synaptic structure and function. Moreover, only 22 genes among the analyzed 38 genetic loci encoding synaptic proteins have more possibility to under the control of non-coding RNA genes. In addition, the distribution of miRNAs along the 3＇ untranslated region （UTR） of these 22 genes exhibits different patterns. Condusion Here we provide the computational screen and analysis results for the genetic loci required for synaptic structure and function and their possible corresponding miRNAs. These data will be useful for the further attempt to systematically determine the roles of miRNAs in synaptic assembly and function regulation in worms.

全站仪三点前方交会测量风电塔架倾斜

倾斜测量是了解高耸构筑物结构安全的一项重要工作,以风电塔架的倾斜观测为例,采用三点前方交会观测塔架倾斜,对交会点的点位精度进行分析,并将塔架倾斜数据与基础倾斜数据相比对。结果表明,三点前方交会法的倾斜数据可靠、测量精度高,能够为高耸构筑物的结构安全提供有力保障。

Unprecedented Retreat in a 50-Year Observational Record for Petermann Glacier, North Greenland

Petermann Glacier is a marine-terminating outlet glacier that had a 70 km-long floating ice tongue prior to a ～ 270 km2 calving event that was observed from satellite sensors in August 2010,shortening the ice tongue by ～ 27 km.Further,in July 2012,another 10 km was lost through calving.In order to understand these events in perspective,here the authors perform a long-term data analysis of Petermann Glacier calving-front variability and ice velocity for each year in the 1990s-2000s,supplemented by available observations from the previous three decades.Five major （on the order of 100 krm2） calving events are identified,with ～ 153 km2 calved from 1959 to 1961,～ 168 km2 in 1991,～ 71 km2 in 2001,～ 270 km2 in 2010,and ～ 130 km2 in 2012-as well as ～ 31 k m2 calved in 2008.The increased frequency of major calving events in recent years has left the front terminus position retreated nearly 25 km beyond the range of observed in previous decades.In contrast,stable ice-dynamics are suggested from ice-velocity measurements made each year between 1993-2012,which are on average 1063 m yr-1,with limited interannual variability and no significant trend; moreover,there is no apparent relationship between ice-velocity variability and calving events.The degree to which the massive calving events in 2010 and 2012 represent natural episodic variability or a response to atmospheric and/or oceanic changes remains speculative; however,melt-induced weakening of the floating ice tongue in recent years is strongly suggested.

Microsatellite-Centromere Mapping in Japanese Scallop(Patinopecten yessoensis) Through Half-Tetrad Analysis in Gynogenetic Diploid Families

Gene-centromere mapping is an essential prerequisite for understanding the composition and structure of genomes. Half-tetrad analysis is a powerful tool for mapping genes and understanding chromosomal behavior during meiosis. The Japanese scallop(Patinopecten yessoensis), a cold-tolerant species inhabiting the northwestern Pacific coast, is a commercially important marine bivalve in Asian countries. In this study, inheritance of 32 informative microsatellite loci was examined in 70-h D-shaped larvae of three induced meiogynogenetic diploid families of P. yessoensis for centromere mapping using half-tetrad analysis. The ratio of gynogenetic diploids was proven to be 100%, 100% and 96% in the three families, respectively. Inheritance analysis in the control crosses showed that 51 of the 53 genotypic ratios observed were in accordance with Mendelian expectations at the 5% level after Bonferroni correction. Seven of the 32 microsatellite loci showed the existence of null alleles in control crosses. The second division segregation frequency(y) of the microsatellite loci ranged from 0.07 to 0.85 with a mean of 0.38, suggesting the existence of positive interference after a single chiasma formation in some chromosomes in the scallop. Microsatellite-centromere distances ranged from 4 c M to 42 c M under the assumption of complete interference. Information on the positions of centromeres in relation to the microsatellite loci will represent a contribution towards the assembly of genetic maps in the commercially important scallop species.

Sensitivity and effect of key operational parameters on performance of a dual-cylinder free-piston engine generator

The free-piston engine generator(FPEG)is regarded as the next generation of energy conversion system which may replace traditional engines in the future.The effect of key operational parameters like excess air ratio of input mixture and ignition position on the engine performance of a dual-cylinder FPEG was investigated,and their sensitivity was analyzed in this paper.The operating compression ratio of the system is susceptible to changes in excess air ratio and ignition position.At the same time,it decreases from 15.8 to 6.6 when excess air ratio increases from 0.85 to 1.15,but it increases from 6.1 to 13.3 as ignition position increases from 15 mm to 20 mm.The operating frequency and indicated power are more sensitive to changes in excess air ratio than ignition position.But it is the opposite for the indicated thermal efficiency and friction loss.Excess air ratio and ignition position have a quite similar influence on heat transfer.Therefore,from the perspective of system operation and performance,it is preferable to keep excess air coefficient slightly below 1.0.In contrast,when selecting ignition position,it is of great importance to comprehensively consider the risk of structural damage caused by the increase in the compression ratio and in-cylinder gas pressure.

Genetic diversity and differentiation of masu salmon(Oncorhynchus masou masou) between and within cultured populations inferred from microsatellite DNA analysis

Masu salmon,Oncorhynchus masou masou,is one of the most valuable fishery species that has been introduced to China,though to date no studies on the genetic diversity and genetic relationship among hatchery populations has been performed with molecular markers.We undertook such a study and sampled 120 individuals from three hatchery stocks and analyzed 20 microsatellite loci.All loci were polymorphic and a total of 91 alleles were detected.A relatively low level of genetic diversity was revealed with effective number of allele of 3.1094,3.3299 and 3.1894 and expected heterozygosity of 0.6600,0.6648 and 0.6638 in the three stocks,respectively.Deviations from Hardy-Weinberg equilibrium were found due to heterozygote deficit.Accordingly,evidence of genetic bottlenecks were found in the three stocks.An individual assignment test demonstrated that 85% of individuals were correctly assigned into their original stocks.Pairwise Fst revealed that significant differentiation occurred between these three stocks.The results of the study indicated that disequilibrium of genetic structure and differentiation has occurred in all three stocks.This information collectively provides a basis for measures to avoid of loss of genetic diversity and introgression in Chinese aquaculture.

Dynamic Analysis of Kineto-Elastic Beam System with Second-order Effect

Dynamic equations of motional flexible beam elements were derived considering second-order effect. Non-linear finite element method and three-node Euler-Bernoulli beam elements were used. Because accuracy is higher in non-linear structural analysis,three-node beam elements are used to deduce shape functions and stiffness matrices in dynamic equations of flexible elements. Static condensation method was used to obtain the finial dynamic equations of three-node beam elements. According to geometrical relations of nodal displacements in concomitant and global coordinate system,dynamic equations of elements can be transformed to global coordinate system by concomitant coordinate method in order to build the global dynamic equations. Analyzed amplitude condition of flexible arm support of a port crane,the results show that second-order effect should be considered in kinetic-elastic analysis for heavy load machinery of big flexibility.

Investigation and Evaluation of Multilevel H-NPC Converter for Electrically Driven Trains

In this paper the operation of a three level H-bridge converter as well as its parallel operations is analyzed and simulated on the computer. Based on the simulation results the operating behavior between （a） a three level H-bridge neutral point clamped convener, （b） a three level back-to-back H-bridge neutral point clamped convener, （c） two three level H-bridge neutral point clamped converters parallel connected is being compared. From the simulation results it is obvious that in the first two cases the ripples, the distortion in primary and secondary winding currents, and the power factor are quite satisfactory and almost identical to each other. In the third case as compared with the first two, it is observed that current harmonics with higher amplitude appear in the primary winding of the transformer.

基于GIS的短距离定向越野点位设计研究

使用ArcGIS软件地理分析功能,通过对地图中不同土地类型进行差异赋值,提取线路要素,获得线路难度值,从而用于统一定向越野比赛点的位设置,辅助定向越野运动员进行训练与赛后复盘。

Autotriploid origin of Carassius auratus as revealed by chromosomal locus analysis

In the Dongting water system, the Carassius auratus(Crucian carp) complex is characterized by the coexistence of diploid forms(2n=100, 2n CC) and polyploid forms. Chromosomal and karyotypic analyses have suggested that the polyploid C. auratus has a triploid(3n=150, 3n CC) and a tetraploid origin(4n=200), respectively. However, there is a lack of direct genetic evidence to support this conclusion. In this paper, analysis of the 5S r DNA chromosomal locus revealed that the 3n CC is of triploid origin. Analysis of the species-specific chromosomal centromere locus revealed that 3n CC individuals possess three sets of C. auratus-derived chromosomes. Our results provide direct cytogenetic evidence suggesting that individuals with 150 chromosomes are of autotriploid origin within the C. auratus complex. It marks an important contribution to the study of polyploidization and the evolution of vertebrates.

Genetic and genomic analyses for economically important traits and their applications in molecular breeding of cultured fish

The traits of cultured fish must continually be genetically improved to supply high-quality animal protein for human consumption.Economically important fish traits are controlled by multiple gene quantitative trait loci(QTL),most of which have minor effects,but a few genes may have major effects useful for molecular breeding.In this review,we chose relevant studies on some of the most intensively cultured fish and concisely summarize progress on identifying and verifying QTLs for such traits as growth,disease and stress resistance and sex in recent decades.The potential applications of these major-effect genes and their associated markers in marker-assisted selection and molecular breeding,as well as future research directions are also discussed.These genetic and genomic analyses will be valuable for elucidating the mechanisms modulating economically important traits and to establish more effective molecular breeding techniques in fish.

Chromosome-scale genomes provide new insights into subspecies divergence and evolutionary characteristics of the giant panda

Extant giant pandas are divided into Sichuan and Qinling subspecies.The giant panda has many speciesspecific characteristics,including comparatively small organs for body size,small genitalia of male individuals,and low reproduction.Here,we report the most contiguous,high-quality chromosomelevel genomes of two extant giant panda subspecies to date,with the first genome assembly of the Qinling subspecies.Compared with the previously assembled giant panda genomes based on short reads,our two assembled genomes increased contiguity over 200-fold at the contig level.Additional sequencing of 25 individuals dated the divergence of the Sichuan and Qinling subspecies into two distinct clusters from 10,000 to 12,000 years ago.Comparative genomic analyses identified the loss of regulatory elements in the dachshund family transcription factor 2(DACH2)gene and specific changes in the synaptotagmin 6(SYT6)gene,which may be responsible for the reduced fertility of the giant panda.Positive selection analysis between the two subspecies indicated that the reproduction-associated IQ motif containing D(IQCD)gene may at least partly explain the different reproduction rates of the two subspecies.Furthermore,several genes in the Hippo pathway exhibited signs of rapid evolution with giant panda-specific variants and divergent regulatory elements,which may contribute to the reduced inner organ sizes of the giant panda.