通过云芝菌发酵得到的漆酶粗酶液经超滤浓缩后,以硫酸铵为盐析剂进行分级盐析沉淀,利用DEAE-Sepharose-F.F.柱层析对漆酶的发酵液进行分离纯化.SDS-PAGE电泳分析证明获得了电泳纯漆酶,其分子量约为64.4 k D.分离纯化过程中酶被纯化了22...通过云芝菌发酵得到的漆酶粗酶液经超滤浓缩后,以硫酸铵为盐析剂进行分级盐析沉淀,利用DEAE-Sepharose-F.F.柱层析对漆酶的发酵液进行分离纯化.SDS-PAGE电泳分析证明获得了电泳纯漆酶,其分子量约为64.4 k D.分离纯化过程中酶被纯化了22.80倍,酶活的回收率为36.32%,表明云芝菌产漆酶得到了很好的分离纯化.将漆酶液用于烟梗木质素的降解,实验结果表明,经此降解处理过的烟梗其内在品质得到了一定的提高:杂气和刺激性明显降低,木质气明显减弱,香气和余味均有改善.展开更多
Lignin only accounts for about 6%of total mass in tobacco stem,but it inuences the harmful substances in the side stream smoke of cigarette in a significant way.Traditional researches focus only on the determination o...Lignin only accounts for about 6%of total mass in tobacco stem,but it inuences the harmful substances in the side stream smoke of cigarette in a significant way.Traditional researches focus only on the determination of lignin content.In the present work,we investigate four typical imidazolium-based ionic liquids for efficient extraction of lignin under mild conditions and 1-ethyl-3-methylimidazolium diethylphosphate([Emim][DEP])shows the best results.The pretreatment of stem using water at 80℃for 30 min can not only remove most of the sugars but also loose the microbers.The extractive rate of lignin reaches 85.38%at 150℃for 4 h and the purity of lignin is 90.21%.展开更多
文摘通过云芝菌发酵得到的漆酶粗酶液经超滤浓缩后,以硫酸铵为盐析剂进行分级盐析沉淀,利用DEAE-Sepharose-F.F.柱层析对漆酶的发酵液进行分离纯化.SDS-PAGE电泳分析证明获得了电泳纯漆酶,其分子量约为64.4 k D.分离纯化过程中酶被纯化了22.80倍,酶活的回收率为36.32%,表明云芝菌产漆酶得到了很好的分离纯化.将漆酶液用于烟梗木质素的降解,实验结果表明,经此降解处理过的烟梗其内在品质得到了一定的提高:杂气和刺激性明显降低,木质气明显减弱,香气和余味均有改善.
基金supported by the China Postdoctoral Science Foundation (BH2060000062)China Tobacco Anhui Industrial Co.,LTD
文摘Lignin only accounts for about 6%of total mass in tobacco stem,but it inuences the harmful substances in the side stream smoke of cigarette in a significant way.Traditional researches focus only on the determination of lignin content.In the present work,we investigate four typical imidazolium-based ionic liquids for efficient extraction of lignin under mild conditions and 1-ethyl-3-methylimidazolium diethylphosphate([Emim][DEP])shows the best results.The pretreatment of stem using water at 80℃for 30 min can not only remove most of the sugars but also loose the microbers.The extractive rate of lignin reaches 85.38%at 150℃for 4 h and the purity of lignin is 90.21%.
