This study almed to analyze the characteristics of Chi I （cIass I chitinase） in tobacco by using bioinformatics methods and tooIs so as to Iay a foundation for further study on disease resistance of Chi I in tobacco...This study almed to analyze the characteristics of Chi I （cIass I chitinase） in tobacco by using bioinformatics methods and tooIs so as to Iay a foundation for further study on disease resistance of Chi I in tobacco. The amino acid sequence characteristics and phyIogenetic reIationships of Chi Is of Nicotiana tabacum, Ara-bidopsis thaliana, Oryza sativa and Phaseolus vulgaris from NCBI were predicted and analyzed by using bioinformatics methods. The resuIts showed that the moIec-uIar weights of Chi Is with signal peptides ranged from 28 to 37 kDa. The se-quences of Chi Is, which al contalned both N-terminal chitin binding domaln （Chit BD1）andcatalyticdomaln（CIyco hydro-19）,werehighIyconserved.Thesequences of Nt-ChiA, Nt-ChiB and Nt-ChiC, three Chi Is from Nicotiana tabacum had high simiIarity, so their phyIogenetic reIationships were also cIose. The reIationships be-tween Chi Is from Nicotiana tabacum and one of the Chi Is from Arabidopsis thaliana and Phaseolus vulgaris were cIoser than those between Chi Is from Nico-tiana tabacum and Oryza sativa. The resuIts indicated that the properties of Chi I in tobacco were almost the same as those in other pIants. This study wiI provide a basis for further researches on disease resistance proteins in tobacco.展开更多
基金Supported by Major Science and Technology Projects of China National Tobacco Corporation(110201301009)Project of Chongqing Branch of China National Tobacco Corporation(NY20140403030022)~~
文摘This study almed to analyze the characteristics of Chi I （cIass I chitinase） in tobacco by using bioinformatics methods and tooIs so as to Iay a foundation for further study on disease resistance of Chi I in tobacco. The amino acid sequence characteristics and phyIogenetic reIationships of Chi Is of Nicotiana tabacum, Ara-bidopsis thaliana, Oryza sativa and Phaseolus vulgaris from NCBI were predicted and analyzed by using bioinformatics methods. The resuIts showed that the moIec-uIar weights of Chi Is with signal peptides ranged from 28 to 37 kDa. The se-quences of Chi Is, which al contalned both N-terminal chitin binding domaln （Chit BD1）andcatalyticdomaln（CIyco hydro-19）,werehighIyconserved.Thesequences of Nt-ChiA, Nt-ChiB and Nt-ChiC, three Chi Is from Nicotiana tabacum had high simiIarity, so their phyIogenetic reIationships were also cIose. The reIationships be-tween Chi Is from Nicotiana tabacum and one of the Chi Is from Arabidopsis thaliana and Phaseolus vulgaris were cIoser than those between Chi Is from Nico-tiana tabacum and Oryza sativa. The resuIts indicated that the properties of Chi I in tobacco were almost the same as those in other pIants. This study wiI provide a basis for further researches on disease resistance proteins in tobacco.
