重症监护病房(intensive care unit,ICU)的危重病人因长期卧床、固定和机械通气(mechanical ventilation,MV)可能导致包括ICU获得性衰弱、认知障碍、心里障碍在内的各种问题。即使在出院后的很长时间内,病人仍将持续遭受这些问题的困扰...重症监护病房(intensive care unit,ICU)的危重病人因长期卧床、固定和机械通气(mechanical ventilation,MV)可能导致包括ICU获得性衰弱、认知障碍、心里障碍在内的各种问题。即使在出院后的很长时间内,病人仍将持续遭受这些问题的困扰,导致身心功能受损,生活质量下降。ICU病人的早期活动与康复(early mobilization and rehabilitation,EMR)被认为可以在一定程度上避免上述问题的发生,缓解重症监护后综合征的不良影响。展开更多
Objective: To investigate the role of intercellular adhesion molecule 1 (ICAM 1) in the accumulation of polymorphonuclear neutrophils (PMN) in the lungs at the early stage of burns. Methods: Myeloperoxidase content in...Objective: To investigate the role of intercellular adhesion molecule 1 (ICAM 1) in the accumulation of polymorphonuclear neutrophils (PMN) in the lungs at the early stage of burns. Methods: Myeloperoxidase content in lung tissues and bronchoalveolar lavage fluid (BALF) were detected. ICAM 1 and its mRNA expression in lung tissues were determined by immunohistochemical method and in situ hybridization. CD11b/CD18 expression on the peripheral PMNs was measured by flowcytometry. Results: The levels of myeloperoxidase in lung tissues and BALF after burn injury were markedly higher than those of control. Expression of ICAM 1 and its mRNA in the lung tissues and CD11b/CD18 on peripheral PMNs surface was significantly increased at 2, 6, 12, 24 h after burns. Conclusions: PMNs accumulation in the lungs is related to increased ICAM 1 expression on pulmonary microvascular endothelial cells and CD11b/CD18 expression on PMN at the early stage of burn injury.展开更多
文摘重症监护病房(intensive care unit,ICU)的危重病人因长期卧床、固定和机械通气(mechanical ventilation,MV)可能导致包括ICU获得性衰弱、认知障碍、心里障碍在内的各种问题。即使在出院后的很长时间内,病人仍将持续遭受这些问题的困扰,导致身心功能受损,生活质量下降。ICU病人的早期活动与康复(early mobilization and rehabilitation,EMR)被认为可以在一定程度上避免上述问题的发生,缓解重症监护后综合征的不良影响。
文摘Objective: To investigate the role of intercellular adhesion molecule 1 (ICAM 1) in the accumulation of polymorphonuclear neutrophils (PMN) in the lungs at the early stage of burns. Methods: Myeloperoxidase content in lung tissues and bronchoalveolar lavage fluid (BALF) were detected. ICAM 1 and its mRNA expression in lung tissues were determined by immunohistochemical method and in situ hybridization. CD11b/CD18 expression on the peripheral PMNs was measured by flowcytometry. Results: The levels of myeloperoxidase in lung tissues and BALF after burn injury were markedly higher than those of control. Expression of ICAM 1 and its mRNA in the lung tissues and CD11b/CD18 on peripheral PMNs surface was significantly increased at 2, 6, 12, 24 h after burns. Conclusions: PMNs accumulation in the lungs is related to increased ICAM 1 expression on pulmonary microvascular endothelial cells and CD11b/CD18 expression on PMN at the early stage of burn injury.