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热休克法诱导虹鳟三倍体技术研究
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作者 车德钊 温海深 +3 位作者 李吉方 张美昭 陈落落 辛苑茹 《水产研究》 2019年第2期63-72,共10页
本文以虹鳟(Oncorhynchus mykiss)为实验对象,采用热休克法诱导虹鳟三倍体,利用流式细胞仪(CytoFLEX S)以及比较红细胞长径对虹鳟倍性进行鉴定;在热休克法诱导虹鳟三倍体的两个正交重复实验中,主要研究了处理的起始时间、温度、持续时... 本文以虹鳟(Oncorhynchus mykiss)为实验对象,采用热休克法诱导虹鳟三倍体,利用流式细胞仪(CytoFLEX S)以及比较红细胞长径对虹鳟倍性进行鉴定;在热休克法诱导虹鳟三倍体的两个正交重复实验中,主要研究了处理的起始时间、温度、持续时间三个因素对于虹鳟三倍体诱导效果的影响,结果显示,在实验设定的起始时间、温度、以及持续时间内,温度大小对发眼率、诱导率以及综合评分影响程度都是最大(R值最大),在发眼率结果中,25℃时有最高的发眼率;在诱导率结果中,25℃、27℃明显高于29℃组的诱导率;在综合评分结果中25℃、27℃明显高于29℃组的综合评分。在起始时间为10~20 min内,起始时间对诱导率没有显著影响,但起始时间为20 min时诱导效果最优。在起始时间与处理强度双因素实验结果中,综合评分高于70.00%的共两组,分别为第一组、第二组,热休克的起始时间都是25 min,作用参数分别为25℃持续20 min、27℃持续15 min,是较为理想的诱导三倍体处理强度参数。 展开更多
关键词 虹鳟 热休克法 三倍体 诱导率 综合评分
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X13-1菌体蛋白对小鼠免疫功能的影响
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作者 刘运德 齐新 《天津师范大学学报(自然科学版)》 CAS 2001年第3期71-72,共2页
研究高蛋白含量的 Fusarium菌株 X1 3- 1的最佳生长条件 ,并从菌丝体中获得了高达 47%的菌体蛋白 ,再用热休克方法使其中 RNA的含量降至 2 %以下 ,使之符合 WHO的标准 .通过动物免疫实验证明 。
关键词 菌体蛋白 Fusarium菌株 免疫功能 X13-1 动物免疫实验 热休克法
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HEAT SHOCK PROTEIN gp96 AND CANCER IMMUNOTHERAPY
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作者 岳培彬 杨树德 黄常志 《Chinese Medical Sciences Journal》 CAS CSCD 2002年第4期251-256,共6页
Heat shock protein gp96 is a highly conserved and monomorphic glycoprotein in the endoplasmic reticulum.It functions as molecular chaperone and can associate with a variety of antigenic peptides noncovalently in vivo ... Heat shock protein gp96 is a highly conserved and monomorphic glycoprotein in the endoplasmic reticulum.It functions as molecular chaperone and can associate with a variety of antigenic peptides noncovalently in vivo and in vitro. Recent studies have indicated that gp96 molecules participate in major histocompatibility complex class I - restricted antigen presentation pathway. Immunization of mice with gp96 preparations isolated from cancer cells can elicit a cancer - specific protective T cell immune response that is recallable, which is a prerequisite for gp96 as a therapeutic vaccine against cancers. The immunogenicity of gp96 molecules has been attributed to the antigenic peptides associated with them. These phenomena provide a new pathway for cancer immunotherapy. The mechanism that the gp96 -peptide complex induces specific immune response and the explorations for gp96 - peptide complex as a therapeutic cancer vaccine are reviewed. 展开更多
关键词 heat shock protein GP96 cancer immunotherapy VACCINE
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Heat shock proteins and immunotherapy
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作者 XinZHAO XueMeiXU GuoxingSONG 《Journal of Microbiology and Immunology》 2005年第1期1-14,共14页
Being one of the most abundant intracellular proteins, heat shock proteins (HSPs) have many housekeeping functions which are crucial for the survival of organisms. In addition, some HSPs are new immunoactive molecules... Being one of the most abundant intracellular proteins, heat shock proteins (HSPs) have many housekeeping functions which are crucial for the survival of organisms. In addition, some HSPs are new immunoactive molecules which play important roles in both adaptive and innate immunity. They could activate CD8 + and CD4 + lymphocytes, induce innate immune response including natural killer (NK) cell activation and cytokine secretion, and induce maturation of dendritic cells (DCs). These characteristics have been used for immunotherapy of various types of cancers and infectious diseases. This review focuses on the main HSP families——HSP70 and 90 families. The mechanism of HSPs′ function in eliciting immune response are elucidated and various forms of HSPs used in immunotherapy are discussed in details. At the end of this review, authors summarize clinical trials related to HSPs and evaluate their clinical efficacy. 展开更多
关键词 Heat shock proteins (HSPs) CANCER Infectious diseases IMMUNOTHERAPY
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Effect of moxibustion on expressions of HSP70 mRNA and protein in gastric cancer-bearing rats 被引量:1
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作者 Tan Jing Lin Ya-ping +4 位作者 Yi Shou-xiang Zhao Huan Peng Zhuo-jun Ouyang Li-zhi Peng Yan 《Journal of Acupuncture and Tuina Science》 CSCD 2019年第6期395-401,共7页
Objective:To observe the effect of moxibustion on the mRNA and protein expressions of heat-shock protein 70(HSP70)in gastric cancer-bearing rats.Methods:A total of 40 healthy Sprague-Dawley(SD)rats were adaptively fed... Objective:To observe the effect of moxibustion on the mRNA and protein expressions of heat-shock protein 70(HSP70)in gastric cancer-bearing rats.Methods:A total of 40 healthy Sprague-Dawley(SD)rats were adaptively fed for one week.The gastric cancer model was prepared by Walker-256 cancer tissue transplantation.After 7 d,10 rats were randomly selected to verify the successful modeling,and the remaining 30 rats were divided into a model group,a moxibustion group and an infrared group by the random number table method,with 10 rats in each group.After enrollment,the moxibustion group received suspended moxibustion at Zhongwan(CV 12),Guan yuan(CV 4)and bilateral Zusanli(ST 36),(the first group of acupoints)on the 1st day,and suspended moxibustion at bilateral Pishu(BL 20)and Weishu(BL 21),(the second group of acupoints)on the 2nd day,20 min each time,once a day.Moxibustion was alternately performed every other day at the two groups of acupoints for 21 d.From the day of en roll me nt,rats in the infrared group were irradiated with the infrared radiatio n at the stomach area on the 1st day,and at the T12-Ti3 interspinous region on the 2nd day,20 min each time,once a day,and the two locations were alternately irradiated every other day for 21 d.During the treatment,rats in the model group were intervened by grasping and fixation without treatment.At the end of the treatment,blood was collected from the inner eye orbit,and the HSP70 expression in peripheral blood was determined by enzyme linked immunosorbent assay(ELISA).Rats were sacrificed,the tumor volume and growth inhibition rate were measured.The position and changes of HSP70 in gastric cancer were observed by streptavidin-perosidase(SP);HSP70 protein expression was determined by ELISA;HSP70 mRNA expression in cancer tissues was determined by reverse transcription-polymerase chain reaction(R「PCR)assay.Results:In comparison of the model group,the volume growth of the gastric cancer in the moxibustion group was significantly restricted(P<0.01);the volume growth inhibition rate in the moxibustion group was 37.93%;the HSP70 expressi on in peripheral blood and the cancer tissues was sign ifica ntly in creased(both P<0.01);the expressio n of HSP70 mRNA and HSP70 content in gastric tumor were both obviously in creased in the moxibustion group(P<0.01);and a large amount of HSP70 was released to the outside of cancer cells in the moxibustion group.In comparison of the model group,the volume growth of the gastric cancer in the infrared group was slightly restricted(P<0.05)with a volume growth inhibition rate of 15.89%;the HSP70 expression in the infrared group was increased significantly in peripheral blood(P<0.01)and in the gastric cancer tissues(P<0.05);more HSP70 was released outside of the cancer cells in the infrared group.In comparison of the infrared group,the volume growth of gastric cancer was more restricted in the moxibustion group(P<0.05)z and the HSP70 expression in the gastric cancer tissues was also higher(P<0.05);more HSP70 was released outside of the cancer cells in the moxibustion group.Conclusion:Moxibusti on and in fra red treatme nt inhibit the gastric can cer growth in the gastric cancer-bearing rats,up-regulate the HSP70 expression in gastric cancer tissues,and promote the production and extracellular release of HSP70,and the effect of moxibustion is more obvious. 展开更多
关键词 Acupuncture-moxibustion Therapy Moxibustion Therapy Moxa Stick Moxibustion NEOPLASMS STOMACH HSP70 Heat-shock Proteins RATS
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