AIM： TO investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasi...AIM： TO investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasis, to identify the hepatocarcinoma-specific biomarkers for the early prediction in diagnosis, and to explore the new drug targets for liver cancer therapy. METHODS： Total proteins from human hepatocarcinoma cell line SMMC-7721 were separated by two-dimensional electrophoresis （2DE）. The silver-stained gel was analyzed by 2DE software Image Master 2D Elite. Interesting protein spots were identified by peptide mass fingerprinting based on matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry （MALDI-TOF-MS） and database searching. RESULTS： We obtained protein profile of human hepatocarcinoma cell line SMMC-7721. Among the twenty-one successfully identified proteins, mitofilin, endoplasmic reticulum protein ERp29, ubiquinol-cytochrome C reductase complex core protein I, peroxisomal enoyl CoA hydratase, peroxiredoxin-4 and probable 3-oxoacid CoA transferase 1 precursor were the six novel proteins identified in human hepatocarcinoma cells or tissues. Specific functions of the identified heat-shock proteins were analyzed in detail, and the results suggested that these proteins might promote tumorigenesis via inhibiting cell death induced by several cancer-related stresses or via inhibiting apoptosis at multiple points in the apoptotic signal pathway. Other identified chaperones and cancer-related proteins were also analyzed.CONCLUSION： Based on the protein profile of SMMC-7721 cells, functional analysis suggests that the identified chaperones and cancer-related proteins have their own pathways to contribute to the tumorigenesis, tumor growth and metastasis of liver cancer. Furthermore, proteomic analysis is indicated to be feasible in the cancer study.展开更多
A numerical analysis based on the boundary element method （BEM） was presented for the hydrodynamic performance of a high skew propeller （HSP） which is employed by an underwater vehicle （UV）. Since UVs operate at...A numerical analysis based on the boundary element method （BEM） was presented for the hydrodynamic performance of a high skew propeller （HSP） which is employed by an underwater vehicle （UV）. Since UVs operate at two different working conditions （surface and submerged conditions）, the design of such a propeller is a cumbersome task. This is primarily due to the fact that the resistance forces as well as the vessel efficiency under these conditions are significantly different. Therefbre, some factors are necessary for the design of the optimum propeller to utilize the power at the mentioned conditions. The design objectives of the optimum propeller are to obtain the highest possible thrust, minimum torque, and efficiency. In the current study, a 5-bladed HSP was chosen for running the UV. This propeller operated at the stern of the UV hull where the inflow velocity to the propeller was non-uniform. Some parameters of the propeller were predicted based on the UV geometrical hull and operating conditions. The computed results include the pressure distribution and the hydrodynamic characteristics of the HSP in open water conditions, and comparison of these results with those of the experimental data indicates good agreement. The propeller efficiency for both submerged and surface conditions was found to be 67% and 64%, respectively, which compared to conventional propellers is a significantly higher efficiency.展开更多
基金Supported by the National Natural Science Foundation of China, No. 30370403the Key Project of Chinese Ministry of Education, No. 705046the Doctoral Foundation of Xi’an Jiaotong University, grants No. DFXJTU2005-05
文摘AIM： TO investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasis, to identify the hepatocarcinoma-specific biomarkers for the early prediction in diagnosis, and to explore the new drug targets for liver cancer therapy. METHODS： Total proteins from human hepatocarcinoma cell line SMMC-7721 were separated by two-dimensional electrophoresis （2DE）. The silver-stained gel was analyzed by 2DE software Image Master 2D Elite. Interesting protein spots were identified by peptide mass fingerprinting based on matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry （MALDI-TOF-MS） and database searching. RESULTS： We obtained protein profile of human hepatocarcinoma cell line SMMC-7721. Among the twenty-one successfully identified proteins, mitofilin, endoplasmic reticulum protein ERp29, ubiquinol-cytochrome C reductase complex core protein I, peroxisomal enoyl CoA hydratase, peroxiredoxin-4 and probable 3-oxoacid CoA transferase 1 precursor were the six novel proteins identified in human hepatocarcinoma cells or tissues. Specific functions of the identified heat-shock proteins were analyzed in detail, and the results suggested that these proteins might promote tumorigenesis via inhibiting cell death induced by several cancer-related stresses or via inhibiting apoptosis at multiple points in the apoptotic signal pathway. Other identified chaperones and cancer-related proteins were also analyzed.CONCLUSION： Based on the protein profile of SMMC-7721 cells, functional analysis suggests that the identified chaperones and cancer-related proteins have their own pathways to contribute to the tumorigenesis, tumor growth and metastasis of liver cancer. Furthermore, proteomic analysis is indicated to be feasible in the cancer study.
基金the Marine Research Center of Amirkabir University of Technology for financial support of thi sresearch
文摘A numerical analysis based on the boundary element method （BEM） was presented for the hydrodynamic performance of a high skew propeller （HSP） which is employed by an underwater vehicle （UV）. Since UVs operate at two different working conditions （surface and submerged conditions）, the design of such a propeller is a cumbersome task. This is primarily due to the fact that the resistance forces as well as the vessel efficiency under these conditions are significantly different. Therefbre, some factors are necessary for the design of the optimum propeller to utilize the power at the mentioned conditions. The design objectives of the optimum propeller are to obtain the highest possible thrust, minimum torque, and efficiency. In the current study, a 5-bladed HSP was chosen for running the UV. This propeller operated at the stern of the UV hull where the inflow velocity to the propeller was non-uniform. Some parameters of the propeller were predicted based on the UV geometrical hull and operating conditions. The computed results include the pressure distribution and the hydrodynamic characteristics of the HSP in open water conditions, and comparison of these results with those of the experimental data indicates good agreement. The propeller efficiency for both submerged and surface conditions was found to be 67% and 64%, respectively, which compared to conventional propellers is a significantly higher efficiency.
