目的磁共振三维高时间分辨力动态增强成像(three-dimenstional time resolved imaging of contrast kinetics,3D-Tricks)技术联合血清白细胞介素6(interleukin-6,IL-6)、热休克蛋白60抗体(antibody to heat shock protein 60,anti-HSP60...目的磁共振三维高时间分辨力动态增强成像(three-dimenstional time resolved imaging of contrast kinetics,3D-Tricks)技术联合血清白细胞介素6(interleukin-6,IL-6)、热休克蛋白60抗体(antibody to heat shock protein 60,anti-HSP60)检测在输卵管性不孕临床诊断中的应用价值。方法选取疑似输卵管性不孕症患者120例作为研究对象,并均行磁共振3D-Tricks技术和血清IL-6、anti-HSP60检测,分为三种检测方式:常规的血清IL-6+anti-HSP60、磁共振3D-Tricks技术、两种检测方式联合,以腹腔镜病理学结果为诊断金标准,分析比较IL-6、anti-HSP60阳性率,评估磁共振3D-Tricks技术联合血清IL-6、anti-HSP60检测在输卵管性不孕临床诊断中的检测诊断合格率、敏感度、特异度。结果120例患者中经腹腔镜病理学确诊有84例,其中联合组诊断符合率(96.43%)显著高于单独组(80.00%),且84例输卵管性不孕患者中血清anti-HSP60、IL-6阳性率均显著高于非输卵管性不孕患者,差异有统计学意义(P<0.05)。磁共振3D-Tricks技术以及血清IL-6、anti-HSP60平行联合检测诊断的敏感度显著高于磁共振3D-Tricks技术、血清IL-6、anti-HSP60单独检测(P<0.05),系列联合检测诊断的特异度显著高于两者的单独检测(P<0.05)。磁共振3D-Tricks技术以及血清IL-6、anti-HSP60联合检测的ROC曲线下面积显著高于单独检测(P<0.05)。结论磁共振3D-Tricks技术联合血清IL-6、anti-HSP60平行联合检测可提高输卵管性不孕临床诊断的敏感度,系列检测可提高输卵管性不孕临床诊断的特异度,对于输卵管性不孕的早期诊断具有重要价值。展开更多
Central Composite Design(CCD) and response surface methodology were used in the experiment to examine the combined effect of temperature(16-28℃) and salinity(18-42) on Hsp70 and IgM genes expression levels in turbot(...Central Composite Design(CCD) and response surface methodology were used in the experiment to examine the combined effect of temperature(16-28℃) and salinity(18-42) on Hsp70 and IgM genes expression levels in turbot(Scophthalmus maximus) liver and kidney.The results showed that the coefficients of determination(R^2=0.965 2 for liver Hsp70,0.972 9 for kidney Hsp70,0.921 for liver IgM and 0.962 1 for kidney IgM) and probability values(P<0.01) were significant for the regression model.The interactive effect between temperature and salinity on liver Hsp70,kidney Hsp70 and liver IgM were not significant(P>0.05),while the interactive effect between temperature and salinity on kidney IgM was significant(P<0.01).The model equation could be used in practice for forecasting Hsp70 and IgM genes expression levels in the liver and kidney of juvenile turbot via applying statistical optimization of the response of interest,at which the maximum liver Hsp70,kidney Hsp70,liver IgM and kidney IgM of1.48,1.49,2.48,and 1.38,respectively,were reached.The present model may be valuable in assessing the feasibility of turbot farming at different geographic locations and,furthermore,could be a useful reference for scientists studying the immunity of turbot.展开更多
文摘目的磁共振三维高时间分辨力动态增强成像(three-dimenstional time resolved imaging of contrast kinetics,3D-Tricks)技术联合血清白细胞介素6(interleukin-6,IL-6)、热休克蛋白60抗体(antibody to heat shock protein 60,anti-HSP60)检测在输卵管性不孕临床诊断中的应用价值。方法选取疑似输卵管性不孕症患者120例作为研究对象,并均行磁共振3D-Tricks技术和血清IL-6、anti-HSP60检测,分为三种检测方式:常规的血清IL-6+anti-HSP60、磁共振3D-Tricks技术、两种检测方式联合,以腹腔镜病理学结果为诊断金标准,分析比较IL-6、anti-HSP60阳性率,评估磁共振3D-Tricks技术联合血清IL-6、anti-HSP60检测在输卵管性不孕临床诊断中的检测诊断合格率、敏感度、特异度。结果120例患者中经腹腔镜病理学确诊有84例,其中联合组诊断符合率(96.43%)显著高于单独组(80.00%),且84例输卵管性不孕患者中血清anti-HSP60、IL-6阳性率均显著高于非输卵管性不孕患者,差异有统计学意义(P<0.05)。磁共振3D-Tricks技术以及血清IL-6、anti-HSP60平行联合检测诊断的敏感度显著高于磁共振3D-Tricks技术、血清IL-6、anti-HSP60单独检测(P<0.05),系列联合检测诊断的特异度显著高于两者的单独检测(P<0.05)。磁共振3D-Tricks技术以及血清IL-6、anti-HSP60联合检测的ROC曲线下面积显著高于单独检测(P<0.05)。结论磁共振3D-Tricks技术联合血清IL-6、anti-HSP60平行联合检测可提高输卵管性不孕临床诊断的敏感度,系列检测可提高输卵管性不孕临床诊断的特异度,对于输卵管性不孕的早期诊断具有重要价值。
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A408-8)the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-50-G01)
文摘Central Composite Design(CCD) and response surface methodology were used in the experiment to examine the combined effect of temperature(16-28℃) and salinity(18-42) on Hsp70 and IgM genes expression levels in turbot(Scophthalmus maximus) liver and kidney.The results showed that the coefficients of determination(R^2=0.965 2 for liver Hsp70,0.972 9 for kidney Hsp70,0.921 for liver IgM and 0.962 1 for kidney IgM) and probability values(P<0.01) were significant for the regression model.The interactive effect between temperature and salinity on liver Hsp70,kidney Hsp70 and liver IgM were not significant(P>0.05),while the interactive effect between temperature and salinity on kidney IgM was significant(P<0.01).The model equation could be used in practice for forecasting Hsp70 and IgM genes expression levels in the liver and kidney of juvenile turbot via applying statistical optimization of the response of interest,at which the maximum liver Hsp70,kidney Hsp70,liver IgM and kidney IgM of1.48,1.49,2.48,and 1.38,respectively,were reached.The present model may be valuable in assessing the feasibility of turbot farming at different geographic locations and,furthermore,could be a useful reference for scientists studying the immunity of turbot.
